Lef1 (BC057543) Mouse Tagged ORF Clone

CAT#: MR205990

Lef1 (Myc-DDK-tagged) - Mouse lymphoid enhancer binding factor 1 (cDNA clone MGC:66504 IMAGE:6401514)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lef1
• Synonyms: 3000002B05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205990 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAACTTTCCGGAGGAGGCGGCGGGGGGGACCCGGAACTCTGCGCCACCGATGAGATGATCCCCT
  TCAAGGACGAAGGCGATCCCCAGAAGGAGAAGATCTTCGCCGAGATCAGTCATCCCGAAGAGGAGGGCGA
  CTTAGCCGACATCAAGTCATCTTTGGTTAACGAGTCCGAAATCATCCCAGCCAGCAACGGGCATGAGGTG
  GTCAGACAAGCCCCGTCCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCTGATGAAGGAAAGC
  ATCCAGACGGAGGCCTGTACAACAAGGGACCCTCCTACTCCAGTTACTCTGGCTACATAATGATGCCCAA
  TATGAACAGCGACCCGTACATGTCAAATGGGTCCCTTTCTCCACCCATCCCGAGGACATCAAATAAAGTG
  CCCGTGGTGCAGCCCTCTCACGCGGTCCACCCGCTCACCCCCCTCATCACCTACAGCGACGAGCACTTTT
  CTCCGGGATCCCACCCGTCACACATCCCGTCAGATGTCAACTCCAAGCAAGGCATGTCCAGACACCCTCC
  AGCTCCTGAAATCCCCACCTTCTACCCCCTGTCTCCGGGCGGCGTTGGACAGATCACCCCACCCATTGGC
  TGGTTTTCCCATCATATGATTCCTGGTCCCCCTGGCCCCCACACAACTGGCATCCCTCATCCAGCTATTG
  TAACACCTCAGGTCAAACAGGAGCACCCCCACACGGACAGTGACCTAATGCACGTGAAGCCTCAACACGA
  ACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGCCTCTGAATGCTTTCATGTTATATATG
  AAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGAGAGTGCAGCTATCAACCAGATCCTGG
  GCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATACTATGAACTAGCACGGAAAGAGAGACA
  GCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATTATGGCAAGAAGAAGAAGAGGAAGAGA
  GAGAAGCTACAGGAGTCGACTTCAGGTGGTAAGAGAAGCTCCTTCCCAACGTGCAAAGCCAAGGCAGCGA
  CCCCAGGCCCTCTTCTGGAGATGGAAGCTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205990 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPQLSGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEV
  VRQAPSSQEPYHDKAREHPDEGKHPDGGLYNKGPSYSSYSGYIMMPNMNSDPYMSNGSLSPPIPRTSNKV
  PVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPEIPTFYPLSPGGVGQITPPIG
  WFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYM
  KEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKR
  EKLQESTSGGKRSSFPTCKAKAATPGPLLEMEAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057543
• ORF Size: 1152 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057543, AAH57543
• RefSeq Size: 2999 bp
• RefSeq ORF: 1154 bp
• Locus ID: 16842
• MW: 42.6 kDa