Serpina10 (BC018416) Mouse Tagged ORF Clone

CAT#: MR206155

Serpina10 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 (cDNA clone

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Serpina10
• Synonyms: MGC25863, ZPI, PZI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206155 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTGGCTTCTAGCCTCTTTCTTCCTGTCCTCCTGACAGAGGTGTGGCTGGTGACTAGTTTCAATC
  TCAGCTCCCATTCACCAGAGGCTTCCGTTCACCTGGAGTCTCAGGATTATGAGAATCAAACCTGGGAAGA
  GTACACACGGACTGATCCCAGGGAGGAGGAGGAGGAGGAGGAGGAAAAGGAGGAAGGCAAGGATGAGGAA
  TATTGGCTGAGGGCCAGTCAGCAGCTCTCCAATGAGACTTCAAGCTTTGGGTTCAACCTGCTTCGAAAGA
  TCTCCATGAGGCACGACGGCAATGTGATCTTCTCACCTTTTGGCCTGTCTGTGGCCATGGTGAATTTGAT
  GCTGGGGACCAAGGGAGAGACCAAAGTCCAGATAGAAAATGGACTCAACCTACAGGCCCTGAGCCAAGCA
  GGACCCCTGATCCTTCCAGCCCTCTTCAAGAAGGTCAAAGAGACCTTCTCCAGCAACCGGGACCTGGGCC
  TCAGCCAGGGTAGCTTTGCCTTCATCCACAAGGACTTTGATATCAAAGAGACCTATTTCAATCTCTCCAA
  GAAGTATTTTGATATAGAGTATGTGTCTATAAATTTTCAAAATTCCTCACAAGCCAGAGGGCTCATAAAC
  CACTGCATTGTCAAAGAGACTGAGGGGAAAATCCCCAAGCTTTTTGATGAGATTAATCCTGAAACAAAGT
  TAATTCTGGTGGACTACGTCTTGTTCAAAGGAAATGCCACCATGCTAGTGGTCCTCATGGAGAAGACAGG
  TGATTACTTGGCCCTGGAGGACTACTTGACCGTGGATCTCGTGGAGACATGGCTGCAGAATATGAAAACC
  AGGAAAATGGAGGTCTTCTTTCCCAAGTTCAAGCTGAACCAGAGGTATGAGATGCACGAGCTGCTCAAAC
  AGATGGGAATCAGGAGGCTCTTCTCCACCTCAGCTGACCTCAGCGAACTCTCAGCCATGGCAAGAAACCT
  TCAGGTGTCCAGGGTTTTACAACAGTCGGTGCTTGAGGTGGATGAAAGGGGAACAGAAGCAGTGTCAGGG
  ACGCTGTCAGAGATTATCGCTTACTCCATGCCTCCTGCCATCAAAGTGAACCGGCCTTTTCACTTCATCA
  TCTATGAGGAGATGTCCAGGATGCTCCTATTTCTGGGCAGGGTGGTAAACCCCACAGTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206155 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRVASSLFLPVLLTEVWLVTSFNLSSHSPEASVHLESQDYENQTWEEYTRTDPREEEEEEEEKEEGKDEE
  YWLRASQQLSNETSSFGFNLLRKISMRHDGNVIFSPFGLSVAMVNLMLGTKGETKVQIENGLNLQALSQA
  GPLILPALFKKVKETFSSNRDLGLSQGSFAFIHKDFDIKETYFNLSKKYFDIEYVSINFQNSSQARGLIN
  HCIVKETEGKIPKLFDEINPETKLILVDYVLFKGNATMLVVLMEKTGDYLALEDYLTVDLVETWLQNMKT
  RKMEVFFPKFKLNQRYEMHELLKQMGIRRLFSTSADLSELSAMARNLQVSRVLQQSVLEVDERGTEAVSG
  TLSEIIAYSMPPAIKVNRPFHFIIYEEMSRMLLFLGRVVNPTVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018416
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018416, AAH18416
• RefSeq Size: 1450 bp
• RefSeq ORF: 1184 bp
• Locus ID: 217847
• MW: 45.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the large serpin family of proteins, and is also known as serpin PZ-dependent protease inhibitor (ZPI or PZI). This protein is thought to play an important role in the regulation of coagulation. It directly inhibits factor XIa, and also inhibits factor Xa in the presence of calcium, phospholipids, and protein Z (PZ). Deficiencies in this gene lead to an increase in thrombosis. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Aug 2014]