Apoa4 (NM_007468) Mouse Tagged ORF Clone

CAT#: MR206177

Apoa4 (Myc-DDK-tagged) - Mouse apolipoprotein A-IV (Apoa4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apoa4
• Synonyms: Apoa-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206177 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTGAAGGCTGCGGTGCTGACCCTGGCCCTGGTGGCCATCACCGGCACCCGGGCTGAGGTCACTT
  CGGACCAGGTGGCCAATGTGGTGTGGGATTACTTTACCCAGCTAAGCAACAATGCCAAGGAGGCTGTAGA
  ACAGTTTCAGAAGACGGATGTCACTCAGCAGCTCAGTACCCTCTTCCAGGACAAACTTGGGGATGCTAGT
  ACGTATGCTGATGGGGTGCACAACAAGCTGGTGCCCTTTGTCGTACAGCTGAGTGGGCATCTAGCCCAGG
  AAACTGAGAGGGTGAAGGAAGAGATCAAGAAGGAGCTGGAGGACCTACGTGACCGCATGATGCCCCATGC
  CAACAAAGTAACCCAGACGTTCGGGGAGAACATGCAGAAGTTGCAGGAGCACCTGAAGCCCTATGCCGTG
  GACCTGCAAGATCAGATCAACACACAGACCCAGGAAATGAAGCTCCAGCTGACCCCATACATCCAGCGCA
  TGCAGACCACGATCAAGGAGAATGTGGACAACCTGCACACCTCGATGATGCCCCTTGCCACCAACTTAAA
  GGACAAGTTTAACAGGAATATGGAAGAGCTCAAGGGGCACCTAACCCCCCGTGCCAACGAGCTGAAGGCC
  ACGATCGACCAGAACCTGGAGGATCTGCGCCGCAGCCTGGCCCCTCTGACGGTGGGCGTGCAGGAGAAAC
  TCAACCATCAGATGGAGGGCCTGGCCTTCCAGATGAAGAAGAACGCGGAGGAGCTCCAGACCAAGGTCTC
  CGCAAAAATCGACCAGCTGCAGAAGAATCTGGCCCCGCTGGTGGAAGACGTGCAGAGCAAGGTGAAGGGC
  AACACGGAAGGGCTGCAGAAGTCTCTGGAAGACCTGAACAGGCAGCTGGAGCAGCAGGTGGAGGAGTTCC
  GACGCACTGTGGAGCCCATGGGAGAGATGTTCAACAAGGCTCTGGTGCAGCAGCTGGAACAGTTCAGACA
  GCAGCTGGGTCCCAATTCGGGGGAGGTGGAAAGCCACTTGAGCTTCCTGGAGAAGAGCCTGAGGGAGAAG
  GTCAACTCCTTTATGAGCACCCTGGAAAAAAAGGGGAGCCCAGACCAGCCTCAAGCCCTCCCCCTCCCGG
  AGCAGGCCCAGGAGCAGGCTCAGGAGCAGGCTCAGGAGCAGGTGCAGCCCAAACCTCTGGAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206177 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFLKAAVLTLALVAITGTRAEVTSDQVANVVWDYFTQLSNNAKEAVEQFQKTDVTQQLSTLFQDKLGDAS
  TYADGVHNKLVPFVVQLSGHLAQETERVKEEIKKELEDLRDRMMPHANKVTQTFGENMQKLQEHLKPYAV
  DLQDQINTQTQEMKLQLTPYIQRMQTTIKENVDNLHTSMMPLATNLKDKFNRNMEELKGHLTPRANELKA
  TIDQNLEDLRRSLAPLTVGVQEKLNHQMEGLAFQMKKNAEELQTKVSAKIDQLQKNLAPLVEDVQSKVKG
  NTEGLQKSLEDLNRQLEQQVEEFRRTVEPMGEMFNKALVQQLEQFRQQLGPNSGEVESHLSFLEKSLREK
  VNSFMSTLEKKGSPDQPQALPLPEQAQEQAQEQAQEQVQPKPLES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007468
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007468.2, NP_031494.2
• RefSeq Size: 1666 bp
• RefSeq ORF: 1188 bp
• Locus ID: 11808
• MW: 45 kDa
• Gene Summary: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. [UniProtKB/Swiss-Prot Function]