Ccne2 (NM_001037134) Mouse Tagged ORF Clone

CAT#: MR206360

Ccne2 (Myc-DDK-tagged) - Mouse cyclin E2 (Ccne2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccne2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206360 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGACGCAGCCGTTTACAAGCTAAGCAACATGCCCAGCCCAACCAGCCAGACTCTCCGCAAGAAA
  CCCAGATAATTCAGGCCAAGAAGAGAAAAACAGCACAGGATGTCAAAAAAAGAAAAGAGGAGATCACCAA
  GAAGCATCAGTATGAGATTAGGAATTGTTGGCCACCTGTACTGTCTGGAGGAATCAGCCCTTGCATTATC
  ATTGAAACACCCCATAAAGAAATAGGAACAAGTGACTTCTCTAGATTTACAAATTACAGATTTAAAAATC
  TTTTTATTAATCCCTCACCTCTGCCAGATTTAAGCTGGGCATGTTCACAGGAGGTTTGGCAAAACATGTT
  ACAAAAGGAAAACAGATACGTGCATGACAAACATTTTCAAGTTCTGCATTCTGACCTGGAACCACAGATG
  AGGTCAATACTTTTAGACTGGCTTTTAGAGGTTTGTGAAGTATACACTCTTCATAGGGAGACATTTTACC
  TTGCCCAAGACTTTTTTGACAGATTTATGTTGACACAAAAGGATGTAAATAAAAATATGCCTCAACTCAT
  TGGGATTACCTCATTGTTCATTGCTTCCAAACTTGAGGAAATCTACGCTCCTAAACTCCAAGAGTTTGCT
  TACGTCACTGATGGTGCTTGCAGTGAAGTAGATATCTTAAAGGTGGAACTCAATATATTAAAGGCTTTAA
  AATGGGAACTTTGTCCAGTAACAGTCATCTCCTGGTTGAATCTTTTTCTTCAAGTTGATGCTGTTAAAGA
  TGTTCCTAAGGTTCTTCTGCCTCAATATTCTCAGGAGACGTTCATCCAGATAGCTCAGCTTTTAGATCTG
  TGCATTCTAGCCATCGACTCTTTAGAATTTCAATACAGAATTCTGGTTGCTGCCGCCTTATGTCATTTTA
  CCTCCATTGAAGTGGTTAAGAAAGCTTCAGGTTTGGAATGGGATGACATCTCGGAATGTGTAGACTGGAT
  GGTGCCTTTTGTTAGTGTTGTAAAAAGTGTGAGTCCAGTGAAGCTGAAGACTTTTAAGAAGATACCCATG
  GAAGATAGACACAATATCCAGACACACACAAATTATTTGGCTTTGCTGAATGAAGTAAACTATGTGAACA
  TCTACAGAAAAGGAGGGCAGCTGTCACCAGTGTGTAATGGAGGCATTATGACACCACCAAAGAGTACTGA
  AAAACCACCAGGAAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206360 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRRSRLQAKQHAQPNQPDSPQETQIIQAKKRKTAQDVKKRKEEITKKHQYEIRNCWPPVLSGGISPCII
  IETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWACSQEVWQNMLQKENRYVHDKHFQVLHSDLEPQM
  RSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDVNKNMPQLIGITSLFIASKLEEIYAPKLQEFA
  YVTDGACSEVDILKVELNILKALKWELCPVTVISWLNLFLQVDAVKDVPKVLLPQYSQETFIQIAQLLDL
  CILAIDSLEFQYRILVAAALCHFTSIEVVKKASGLEWDDISECVDWMVPFVSVVKSVSPVKLKTFKKIPM
  EDRHNIQTHTNYLALLNEVNYVNIYRKGGQLSPVCNGGIMTPPKSTEKPPGKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037134
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037134.1, NM_001037134.2, NP_001032211.1
• RefSeq Size: 3113 bp
• RefSeq ORF: 1215 bp
• Locus ID: 12448
• Cytogenetics: 4 A1
• MW: 46.6 kDa
• Gene Summary: Essential for the control of the cell cycle at the late G1 and early S phase. [UniProtKB/Swiss-Prot Function]