Slc16a10 (BC052877) Mouse Tagged ORF Clone

CAT#: MR206388

Slc16a10 (Myc-DDK-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 10 (cDNA clone MGC:60866

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc16a10
• Synonyms: TAT1, PRO0813, 9830169E08
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206388 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCAAGACAGCGTGGGTGGGCTCGCTGTCCATGGGCATGATCTTCTTCTGCTGCCCCATCGTGA
  GTGTCTTCACGGACATGTTCGGCTGCCGGAGAACAGCTGTTGTGGGGGCAGCTGTGGGATTCATTGGACT
  CATGTCCAGTTCTTTTGTAAGCTCCATCGAGCCTCTGTACCTCACCTATGGAATCATATTTGCCTGCGGC
  TGCTCCTTTGCCTACCAGCCGTCACTGGTCATTTTGGGACACTACTTCAAGAAGCGCCTTGGACTAGTCA
  ACGGCATCGTCACGGCCGGCAGCAGCGTCTTCACAATCCTGCTCCCTTTGCTGTTAGGAAATCTAATCAG
  CAGTGTGAAGCTCTTTAACACGCTGCGGATCCTCTGCATCTTCATGTTTGTTCTCTTTCTGGCTGGCTTT
  ACCTACCGACCTCTTGTTCCCAGCACCAAAGAGAAAGAGAGTGGGGGCAGCAGAAGCTCATTCTTCTCCA
  GGAGAAAGCTTAGTCCTCCAAAAAAAGTCTTTAACTTTGCCCTCTTCAAGGAGACAACCTATGCAGTGTG
  GGCTGCTGGGATACCACTTGCACTTTTTGGCTACTTTGTGCCTTACGTTCACTTGATGAACCATGTAAAG
  GAAAGGTTTCAAGATGTAAACAACAAAGAGGTGCTCTTCATGTGCATTGGCATCACTTCAGGAGTTGGAC
  GGCTTCTCTTTGGCCGCATTGCTGACTATTTGCCCGGTGTGAAGAAGGTCTACCTCCAGGTTCTCTCCTT
  CTTCTTCATTGGCCTGATGTCCATGATGATTCCCCTGTGCAGCGCCTTCGGAGCCCTCATTGCTGTCTGC
  CTTGCCATGGGTCTCTTCGATGGATGCTTCATTTCTATCATGGCGCCCATTGCCTTTGAACTAGTTGGTC
  CTCAGGATGCTTCCCAAGCAATTGGATTTCTACTTGGATTCATGTCGATACCCATGACTGTAGGCCCTCC
  TATTGCAGGGTTACTTCATGACGAGCTGGGCACCTACGACGTGGCCTTTTACCTTGCTGGAATCCCTCCC
  TTCGTTGGGGGTGTTGTGCTCTGTTTAATCCCGTGGATCCATAGCAAGAAGCAAAGAAAGATCAGTAAGA
  ACGCCGGAGGGGAGAAGATGGAGAAAATGCTGGAGAACCAGAGCTCTCTACTGTCAGGCTCATCAGGAAT
  CTTCAAAAAAGACTCCGCCTCGATTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206388 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFKTAWVGSLSMGMIFFCCPIVSVFTDMFGCRRTAVVGAAVGFIGLMSSSFVSSIEPLYLTYGIIFACG
  CSFAYQPSLVILGHYFKKRLGLVNGIVTAGSSVFTILLPLLLGNLISSVKLFNTLRILCIFMFVLFLAGF
  TYRPLVPSTKEKESGGSRSSFFSRRKLSPPKKVFNFALFKETTYAVWAAGIPLALFGYFVPYVHLMNHVK
  ERFQDVNNKEVLFMCIGITSGVGRLLFGRIADYLPGVKKVYLQVLSFFFIGLMSMMIPLCSAFGALIAVC
  LAMGLFDGCFISIMAPIAFELVGPQDASQAIGFLLGFMSIPMTVGPPIAGLLHDELGTYDVAFYLAGIPP
  FVGGVVLCLIPWIHSKKQRKISKNAGGEKMEKMLENQSSLLSGSSGIFKKDSASII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052877
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052877, AAH52877
• RefSeq Size: 1454 bp
• RefSeq ORF: 1220 bp
• Locus ID: 72472
• MW: 44.2 kDa