Tead2 (BC050217) Mouse Tagged ORF Clone

CAT#: MR206438

  • TrueORF®

Tead2 (Myc-DDK-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393)


  "BC050217" in other vectors (4)


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Reconstitution Protocol

USD 250.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tead2
Synonyms ETF, TEF4, TEAD-2, TEF-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206438 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGATCCCCGGACTGGGGCCCCCCTGGATGATGGCGGGGGCTGGACAGGTAGCGAGGAAGGCAGCG
AAGAGGGCACCGGAGGCAGCGAGGGCGTTGGAGGTGATGGCAGCCCTGATGCAGAAGGCCGGAACGAACT
CATTGCCCGTTACATCAAGCTGAGGACAGGGAAGACGAGAACGCGAAAGCAGGTCTCCAGCCATATTCAG
GTTTTGGCTCGAAGGAAATCGAGAGAAATTCAGTCCAAGCTGAAGGACCAAGTCTCCAAGGACAAGGCCT
TCCAGACGATGGCCACCATGTCTTCGGCACAGCTCATCTCCGCCCCTTCCCTCCAGGCCAAGCTGGGCCC
TTCTGGCCCTCAGGCCACTGAGCTTTTCCAGTTCTGGTCAGGGAGCTCTGGGCCACCATGGAATGTTCCA
GACGTGAAGCCCTTCTCACAGGCACCGTTCTCCGTGTCACTGACGCCCCCAGCCTCTGACCTACCAGGGT
ACGAGCCGCCCCCAGCCCTCTCACCCCTGCCCCCACCCGCTCCGTCTCCCCCAGCCTGGCAGGCTCGGGC
CCTGGGCACTGCCCGCCTGCAGCTGATAGAGTTCTCAGCGTTTGTGGAACCGCCAGACGCAGTTGACTCG
TTCCAGAGGCATCTGTTTGTCCACATCAGTCAGCAGTGTCCCAGCCCTGGAGCACCACCCCTAGAGAGTG
TGGACGTGCGGCAGATCTACGACAAATTCCCTGAGAAGAAGGGCGGCCTCCGCGAGCTGTATGACCGAGG
GCCACCACATGCCTTCTTCCTCGTCAAGTTCTGGGCGGACCTGAACTGGGGCCCCAGTGCCGAGGAGGCA
GGGAGCAGCGGAGGTGGCGGTGGCTTCTATGGAGTGAGCAGCCAGTATGAGAGCCGGGAGCTCATGACAC
TCACCTGCTCCTCCAAGGTCTGCTCCTTTGGCAAGCAAGTGGTAGAGAAGGTGGAGACGGAACGGGCCCA
GCTGGAGGACGGGCGCTTTGTGTACCGTCTGCTGCGCTCTCCCATGTGTGAGTACCTGGTTAATTTCCTG
CACAAGCTCCGTCAGCTGCCTGAACGCTACATGATGAACAGTGTCCTGGAGAACTTCACCATCCTCCAGG
TTGTGACAAACAGGGACACTCAGGAACTGCTGCTGTGTACTGCCTACGTCTTTGAAGTCTCCACCAGTGA
ACGAGGAGCCCAGTACCACATCTACCGCCTGGTCAGGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206438 protein sequence
Red=Cloning site Green=Tags(s)

MGDPRTGAPLDDGGGWTGSEEGSEEGTGGSEGVGGDGSPDAEGRNELIARYIKLRTGKTRTRKQVSSHIQ
VLARRKSREIQSKLKDQVSKDKAFQTMATMSSAQLISAPSLQAKLGPSGPQATELFQFWSGSSGPPWNVP
DVKPFSQAPFSVSLTPPASDLPGYEPPPALSPLPPPAPSPPAWQARALGTARLQLIEFSAFVEPPDAVDS
FQRHLFVHISQQCPSPGAPPLESVDVRQIYDKFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSAEEA
GSSGGGGGFYGVSSQYESRELMTLTCSSKVCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFL
HKLRQLPERYMMNSVLENFTILQVVTNRDTQELLLCTAYVFEVSTSERGAQYHIYRLVRD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC050217
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC050217, AAH50217
RefSeq Size 1951 bp
RefSeq ORF 1232 bp
Locus ID 21677
MW 45.1 kDa

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