Smarce1 (BC047141) Mouse Tagged ORF Clone

CAT#: MR206466

Smarce1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smarce1
• Synonyms: Baf27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206466 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAAGACCATCTTATGCCCCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGCACACCAG
  GGTTTGTGGGATACAATCCATACAGTCATCTCGCCTACAACAACTACAGGCTGGGAGGGAACCCGGGCAC
  CAACAGCCGGGTCACGGCGTCCTCTGGCATTACGATTCCAAAGCCTCCAAAGCCACCAGATAAGCCGCTG
  ATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGACCAAGTAAAGGCTTCCAACCCTGACCTAAAGTTGT
  GGGAGATTGGCAAGATTATTGGTGGCATGTGGCGAGATCTCACTGATGAAGAGAAGCAAGAATATTTAAA
  CGAATACGAAGCAGAAAAGATAGAGTACAATGAGTCTATGAAGGCCTACCATAATTCCCCTGCGTACCTT
  GCCTATATTAATGCAAAAAGTCGTGCGGAAGCTGCATTAGAGGAAGAAAGTCGACAGAGACAGTCTCGCA
  TGGAGAAAGGAGAACCTTACATGAGCATTCAGCCTGCTGAGGATCCAGACGACTATGATGATGGCTTTTC
  AATGAAGCACACAGCCACTGCCCGTTTCCAGAGAAACCACCGTCTCATCAGTGAGATCCTCAGTGAGAGT
  GTGGTACCTGATGTGCGGTCGGTTGTCACAACAGCTAGAATGCAGGTCCTCAAGCGACAGGTCCAGTCTT
  TAATGGTTCATCAGCGGAAACTAGAAGCCGAGCTCCTTCAGATAGAGGAACGACACCAGGAAAAGAAGAG
  GAAATTCCTGGAAAGCACGGACTCCTTTAACAATGAACTTAAAAGGTTATGTGGTCTGAAGGTAGAAGTA
  GACATGGAGAAGATTGCGGCTGAGATCGCACAGGCGGAGGAACAGGCCCGCAAGAGGCAAGAAGAGAGGG
  AGAAGGAGGCAGCAGAGCAAGCTGAGCGCAGTCAGAGCAGCATGGCCCCTGAGGAAGAGCAAGTGGCGAA
  CAAAGCCGAGGAGAAGAAAGATGAGGAGAGCATCCCGATGGAGACAGAGGAGACACACCTTGAAGACACA
  GCAGAGAGCCAGCAGAATGGTGAAGAAGGCACGTCTACTCCTGAGGACAAGGAGAGTGGGCAGGAGGGGG
  TTGACAGCATGGAGGTGGAAGGGACCAGTGACAGTAACACGGGCTCAGAGAGCAACAGCGCCACAGTGGA
  GGAGCCGCCCACAGACCCAGTGCCAGAAGACGAGAAGAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206466 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPL
  MPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYL
  AYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSES
  VVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEV
  DMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSMAPEEEQVANKAEEKKDEESIPMETEETHLEDT
  AESQQNGEEGTSTPEDKESGQEGVDSMEVEGTSDSNTGSESNSATVEEPPTDPVPEDEKKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC047141
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC047141, AAH47141
• RefSeq Size: 2355 bp
• RefSeq ORF: 1235 bp
• Locus ID: 57376
• MW: 46.6 kDa