Tank (NM_001164072) Mouse Tagged ORF Clone

CAT#: MR206499

Tank (Myc-DDK-tagged) - Mouse TRAF family member-associated Nf-kappa B activator (Tank), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tank
• Synonyms: C86182; E430026L09Rik; I-TRAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206499 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAAACATTGGTGAGCAACTCAATAGAGCATATGAAGCCTTCCGACAGGCATGCATGGATAGAG
  ATTCAGCAGTAAGAGAGCTACAGCAAAAGACTGAGAACTATGAACAAAGAATACGCGAGCAACAGGAACA
  GCTGTCATTTCAACAAAACCTAATTGACAGGCTGAAATCACAGCTACTTCTCGTGGATTCTAGTCGAGAT
  AACAGTTATGGCTATGTACCTTTGCTTGAAGACAGTGACAGAAGGAAGAATAATTTGACCCTTGATGAAC
  CACATGATAAAGTGAAACTAGGAACACTGAGATATAAGCAATCAAAGGTGAGACGACAAGAAGTTTCTTC
  TGGAAAAGAATCCGCCAAGGGTCTCAACATCCCTCTGCATCACGAAAGGGATAATATAGAGAAGACTTTC
  TGGGACCTTAAAGAAGAATTTCATAGGATTTGCTTGCTAGCAAAAGCACAGAAAGACCACTTAAGCAAAC
  TTAATATACCAGATATTGCAACTGACACACAGTGTTCTGTGCCTATACAGTGTACTGATAAAACAGAGAA
  ACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATGATATAAATAGAGGTATGTCGTGCGTCACAGCTGTC
  ACACCAAGAGGACTGGGCCGGGATGAGGAAGATACCTCTTTTGAATCACTTTCTAAATTCAATGTCAAGT
  TTCCGCCTATGGACAATGACTCTATTTTTCTACATAGCACTCCAGAGGCCCCAAGCATCCTTGCTCCTGC
  CACACCTGAGACAGTGTGCCAGGACCGATTTAATATGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAA
  ACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAACTTCAGCTATACAAAACCTTAAAACAA
  CTGACAAAACAAACCCCTCAAATCTTAGAGCGCCGTGTTTGCCAGCTGGAGACCACAATGTGTTCTATGT
  AAATACGTTCCCACTTCAAGACCCGCCTGACGCACCTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTC
  CGAGGACCACAGCAGCCCTTTTGGAAGCCTTTTCTTAACCAAGACACTGACTTAGTGGTACCAAGTGATT
  CAGACTCAGAGCTCCTTAAACCTCTAGTGTGTGAATTCTGTCAAGAGCTTTTCCCACCATCCATTACATC
  CAGAGGGGATTTCCTCCGGCATCTTAATACACACTTTAATGGGGAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206499 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDKNIGEQLNRAYEAFRQACMDRDSAVRELQQKTENYEQRIREQQEQLSFQQNLIDRLKSQLLLVDSSRD
  NSYGYVPLLEDSDRRKNNLTLDEPHDKVKLGTLRYKQSKVRRQEVSSGKESAKGLNIPLHHERDNIEKTF
  WDLKEEFHRICLLAKAQKDHLSKLNIPDIATDTQCSVPIQCTDKTEKQEALFKPQAKDDINRGMSCVTAV
  TPRGLGRDEEDTSFESLSKFNVKFPPMDNDSIFLHSTPEAPSILAPATPETVCQDRFNMEVRDNPGNFVK
  TEETLFEIQGIDPITSAIQNLKTTDKTNPSNLRAPCLPAGDHNVFYVNTFPLQDPPDAPFPSLDSPGKAV
  RGPQQPFWKPFLNQDTDLVVPSDSDSELLKPLVCEFCQELFPPSITSRGDFLRHLNTHFNGET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164072
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164072.1, NP_001157544.1
• RefSeq Size: 2019 bp
• RefSeq ORF: 1242 bp
• Locus ID: 21353
• MW: 47.1 kDa