Mknk1 (NM_021461) Mouse Tagged ORF Clone

CAT#: MR206550

Mknk1 (Myc-DDK-tagged) - Mouse MAP kinase-interacting serine/threonine kinase 1 (Mknk1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mknk1
• Synonyms: 2410048M24Rik; Mnk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206550 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCAGTGAGCCCCTTCCCATCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGG
  CCACCGACTCTCTGCCAGGAAAGTTTGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGC
  CTATGCCAAAGTCCAGGGTGCTGTGAACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAG
  CAAGCCGGGCACAGTCGAAGTCGAGTGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGA
  ACATTTTGGAGCTGATTGAATTCTTTGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGG
  AGGCTCCATCCTAGCACACATCCAGAAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGG
  GACGTCGCCACTGCCCTTGACTTCCTGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACA
  TACTGTGTGAATCTCCAGAAAAGGTGTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAA
  GTTGAACAACTCCTGCACTCCCATAACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATG
  GCACCTGAGGTGGTGGAGGTCTTTAGGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCC
  TGGGTGTGGTCCTCTACATCATGCTGAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGG
  CTGGGACCGGGGAGAGGTATGCAGGATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATAC
  GAGTTTCCTGACAAAGACTGGGCTCACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTC
  GAGATGCGAAGCAAAGACTCAGTGCTGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGA
  AAGGGGACTCCCCACGCCGCAAGTCCTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCT
  GAGGCCATTGCCCTGAACCGCCAGCTGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCC
  TAGCTGAGGGCCTCTGCTCCATGAAGCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCT
  GGCCCAGGCTGGCCGAAGCCGAGATGCAAACCCATGTTTGACACCGGCAGGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206550 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSSEPLPIVDSDKRRKKKRKTRATDSLPGKFEDVYQLTSELLGEGAYAKVQGAVNLQSGKEYAVKIIEK
  QAGHSRSRVFREVETLYQCQGNRNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKRKHFNEREASRVVR
  DVATALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYM
  APEVVEVFRDEATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRMCQNKLFESIQEGKY
  EFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSSTMDLTLFAA
  EAIALNRQLSQHEENELAEEQEALAEGLCSMKLSPPSKSRLARRRALAQAGRSRDANPCLTPAGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021461
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021461.1, NM_021461.2, NM_021461.3, NM_021461.4, NM_021461.5, NP_067436.1
• RefSeq Size: 2554
• RefSeq ORF: 1248
• Locus ID: 17346
• MW: 46.5 kDa
• Gene Summary: This gene encodes a serine-threonine protein kinase that is activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases, and it may function in cytokine and environmental stress responses. This kinase is required for phosphorylation of eukaryotic translation initiation factor 4E but it is not required for cell growth during development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013]