Dclre1b (NM_001025312) Mouse Tagged ORF Clone

CAT#: MR206564

Dclre1b (Myc-DDK-tagged) - Mouse DNA cross-link repair 1B, PSO2 homolog (S. cerevisiae) (Dclre1b), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclre1b
• Synonyms: AI452214; Apollo; mSNM1B; SNMIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206564 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGGAACCTGCTCTGATACTGGGGAAACAGATTCATACTTTGTATCTAGACAACACTAATTGCA
  ATCCAGCCCTGGTTCTTCCTTCCCGACAAGAGGCTACTCAGCAGATTGTCCAGCTAATCCGACAGTTCCC
  ACAACACAACATAAAAATTGGACTCTATAGTCTAGGAAAAGAATCACTGCTGGAGCAACTAGCCCTTGAG
  TTTCGGACCTGGGTGGTATTAAGTCCTCAACGCCTGGAGTTGGTACAGTTGCTGGGCCTGGCAGACGTGT
  TCACAGTTGAGGAAGAAGCCGGGCGCATCCATGCTGTGGACCATACGGAGATCTGCCACTCTGCCATGCT
  TCAGTGGAACCAGAGTCACCCTACCATTGCTATTTTCCCCACAAGCCGGAAAGTGCGCAGCCCTCACCCC
  AGCATCTACACCGTCCCGTACTCTGACCATTCATCTTACTCCGAGCTCCGTGCTTTTGTGGCAGCGCTGA
  GGCCTTGTCAGGTGGTGCCCATCGTCCATCAAAAGCCTTGTGGAGAGTTCTTTCAGGACAGCTTGAGTCC
  TAGGCTCGCTATGCCTCTGATTCCACACTCTGTGCAGCAGTACATGAGTTCCTCCTCTAGGAAAACAAAT
  GTGCTTTGGCAGTTAGAAAGGAGGCTCAAGAGGCCAAGAACTCAGGGTGTTGTGTTTGAATCCCCTGAGG
  AGAAAGCTAATCAGGTTAAAGTTGACAGAGACTCAAAGAAGCACAAAAAGGAAAACCTCTCTCCCTGGGC
  TGGGCACCTTGAGAGGCTTTGCCCCCATCCTCTGCAGGCCAGGAAGCAGTTGTTCCCAGACTTCTGCAGG
  AAAGAACGTGATGAGCCAGTCCTCTTTTGTGACTCCAACAAGATGGCGACTGTGTTGACTGCCCCACTGG
  AATTCTCAGTGCAATTACAACCTATAGATGAGTTTCTCTTTCCAGAAACCAGGGAGAAAATTGGCTTAGA
  GTCCCCATTGCTGTCAAGGGGAGACAGTGGCTCACCAGCGAGAGGGAACCAAAGTGACTGCGTAGGCTGT
  GGTTCTCCCCCGGCTCACATTAGCAGAGCTGTTCCTCTAACTCCTGAGTCCAGGGGCCTGGCGCTAAAAT
  ACCTTCTCACTCCAGTGCATTTCCTCCAAGCAGGCTTCTCTTCCAGGAACTTCGACAAGCAAGTGGAAAA
  ACACCAGAGAGTACAGCGTAGCAGTCCTGCAGTTCTGAGCCCGGTGGATGTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206564 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKEPALILGKQIHTLYLDNTNCNPALVLPSRQEATQQIVQLIRQFPQHNIKIGLYSLGKESLLEQLALE
  FRTWVVLSPQRLELVQLLGLADVFTVEEEAGRIHAVDHTEICHSAMLQWNQSHPTIAIFPTSRKVRSPHP
  SIYTVPYSDHSSYSELRAFVAALRPCQVVPIVHQKPCGEFFQDSLSPRLAMPLIPHSVQQYMSSSSRKTN
  VLWQLERRLKRPRTQGVVFESPEEKANQVKVDRDSKKHKKENLSPWAGHLERLCPHPLQARKQLFPDFCR
  KERDEPVLFCDSNKMATVLTAPLEFSVQLQPIDEFLFPETREKIGLESPLLSRGDSGSPARGNQSDCVGC
  GSPPAHISRAVPLTPESRGLALKYLLTPVHFLQAGFSSRNFDKQVEKHQRVQRSSPAVLSPVDVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025312
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025312.1, NP_001020483.1
• RefSeq Size: 4143 bp
• RefSeq ORF: 1248 bp
• Locus ID: 140917
• Cytogenetics: 3 F2.2
• MW: 46.9 kDa