Stk26 (NM_133729) Mouse Tagged ORF Clone

CAT#: MR206585

2610018G03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2610018G03 gene (2610018G03Rik)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk26
• Synonyms: 2610018G03Rik; Mst4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206585 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACTCACCGGTGGCTGTTCAAGTGCCTGGGATGCAGAATAATATAGCAGATCCAGAAGAACTGT
  TCACAAAATTAGAGCGCATTGGAAAAGGCTCCTTTGGAGAAGTTTTCAAAGGAATTGATAACCGTACTCA
  GCAAGTGGTTGCAATTAAAATCATTGACCTTGAGGAAGCTGAGGATGAAATAGAAGACATCCAACAAGAA
  ATAACTGTTTTGAGTCAGTGCGACAGCTCATATGTAACAAAATACTATGGGTCCTATTTAAAGGGTTCAA
  AACTATGGATAATAATGGAATACCTAGGTGGAGGTTCAGCATTGGATCTTCTGCGTGCTGGTCCATTTGA
  TGAGTTCCAGATTGCCACCATGCTCAAGGAGATTTTGAAAGGTCTGGACTATCTACATTCTGAAAAGAAA
  ATCCACCGAGACATTAAAGCTGCCAACGTCTTGCTTTCAGAACAAGGTGATGTTAAACTGGCTGACTTTG
  GAGTTGCTGGCCAGCTGACAGATACACAAATCAAAAGAAACACCTTCGTAGGGACTCCGTTTTGGATGGC
  TCCTGAAGTTATTCAACAGTCAGCTTATGACTCTAAAGCTGACATATGGTCTTTGGGAATTACTGCTATT
  GAACTTGCCAAGGGAGAGCCTCCGAATTCTGACATGCATCCAATGAGAGTTCTGTTTCTTATTCCAAAAA
  ACAACCCTCCAACTCTTATTGGAGACTTTACTAAGTCTTTCAAGGAGTTTATTGATGCTTGCCTGAATAA
  AGACCCGTCATTTCGTCCTACAGCTAAAGAACTTTTGAAGCATAAGTTCATCGTAAAAAATTCAAAGAAG
  ACTTCTTATCTGACTGAATTGATCGATCGATTTAAGAGATGGAAGGCAGAAGGCCACAGTGATGAGGAAT
  CTGATTCCGAGGGCTCTGACTCGGAATCCAGCAGCAGGGAAAGTAACCCTCACCCTGAATGGAGTTTCAC
  CACTGTGCGTAAGAAGCCTGATCCAAAGAAACTGCAGAATGGGGAAGAGCAAGATCTTGTGCAAACCTTG
  AGCTGTTTGTCTATGATAATCACACCTGCATTTGCCGAACTTAAACAGCAGGACGAGAATAATGCGAGTC
  GAAACCAGGCAATTGAAGAACTTGAGAAAAGTATTGCTGTGGCTGAAACCGCCTGTCCTGGCATCACAGA
  TAAGATGGTGAAGAAACTAATCGAAAAATTTCAAAAGTGTTCTGCGGATGAATCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206585 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHSPVAVQVPGMQNNIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQE
  ITVLSQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKK
  IHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAI
  ELAKGEPPNSDMHPMRVLFLIPKNNPPTLIGDFTKSFKEFIDACLNKDPSFRPTAKELLKHKFIVKNSKK
  TSYLTELIDRFKRWKAEGHSDEESDSEGSDSESSSRESNPHPEWSFTTVRKKPDPKKLQNGEEQDLVQTL
  SCLSMIITPAFAELKQQDENNASRNQAIEELEKSIAVAETACPGITDKMVKKLIEKFQKCSADESP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133729
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133729.1, NM_133729.2, NP_598490.1
• RefSeq Size: 2016 bp
• RefSeq ORF: 1251 bp
• Locus ID: 70415
• MW: 46.6 kDa