Prim1 (NM_008921) Mouse Tagged ORF Clone

CAT#: MR206605

Prim1 (Myc-DDK-tagged) - Mouse DNA primase, p49 subunit (Prim1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prim1
• Synonyms: AI324982
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206605 representing NM_008921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCATTTGATCCTGCGGAGCTGCCGGAGCTACTCAAGCTTTATTACCGAAGGCTCTTTCCCTACG
  CCCAGTACTATCGCTGGCTCAACTATGGCGGAGTGACAAAGAATTACTTTCAACACCGTGAATTTTCATT
  CACACTGAAAGATGATATTTACATTCGCTACCAGTCCTTCAACAACCAGAGTGAACTGGAGAAGGAGATG
  CAGAAAATGAATCCATATAAGATTGATATAGGCGCAGTATATTCTCACAGACCCAATCAACACAATACAG
  TGAAGCTGGGAGCTTTCCAGGCCCAGGAGAAAGAACTGGTCTTTGACATCGACATGACAGACTACGATGA
  CGTAAGGAGATGCTGCAGTTCTGCAGACATATGTTCCAAATGCTGGACTCTCATGACAATGGCCATGCGC
  ATCATAGACCGGGCATTGAAGGAGGACTTTGGATTTAAGCACCGCCTCTGGGTCTATTCAGGAAGGAGAG
  GTGTGCACTGTTGGGTCTGTGATGAATCCGTTCGAAAGCTGTCCTCTGCTGTGCGCTCCGGGATCGTGGA
  GTACTTGAGTCTTGTAAAGGGTGGTCAAGATGTTAAAAAGAAAGTTCACCTAAATGAGAAAGTTCACCCT
  TTTGTCAGAAAATCTATAAACATAATTAAAAAGTACTTTGAGGAATATGCCTTAGTCGGCCAAGATATTC
  TTGAAAATAAAGAAAACTGGGATAAGATTTTAGCCCTTGTTCCTGAGACAATTCATGATGAGCTGCAAAG
  AGGGTTCCAGAAGTTCCACAGTTCACCTCAGCGCTGGGAACACCTGAGGAAAGTGGCCAACTCATCTCAG
  AATATGAAGAACGATAAGTGCGGCCCCTGGCTGGAGTGGGAGGTCATGCTCCAGTACTGTTTCCCACGCC
  TGGATGTCAACGTCAGCAAGGGAGTCAATCACTTACTGAAGAGCCCTTTCAGTGTTCATCCCAAGACAGG
  TCGGATTTCTGTGCCTATCGATTTTCATAAAGTGGATCAATTTGATCCGTTTACTGTCCCAACTATAAGT
  GCCATCTGCCGGGAATTGGATATGGTTTCCACTCATGAGAAGGAAAAGGAGGAGAATGAAGCTGACAGCA
  AACACAGAGTCAGAGGTTACAAGAAGACCAGTCTAGCACCGTATGTGAAAGTATTTGAACAGTTTCTTGA
  AAACCTGGATAAATCCCGGAAAGGGGAACTTCTCAAGAAGAGCGATTTACAAAAAGATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206605 representing NM_008921
  Red=Cloning site Green=Tags(s)
  
  MEPFDPAELPELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHREFSFTLKDDIYIRYQSFNNQSELEKEM
  QKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMR
  IIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEKVHP
  FVRKSINIIKKYFEEYALVGQDILENKENWDKILALVPETIHDELQRGFQKFHSSPQRWEHLRKVANSSQ
  NMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTIS
  AICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENLDKSRKGELLKKSDLQKDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008921
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008921.1, NM_008921.2, NP_032947.1
• RefSeq Size: 1533
• RefSeq ORF: 1254
• Locus ID: 19075
• MW: 49.3 kDa
• Gene Summary: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. [UniProtKB/Swiss-Prot Function]