Pgk1 (BC083355) Mouse Tagged ORF Clone

CAT#: MR206611

Pgk1 (Myc-DDK-tagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pgk1
• Synonyms: Pgk-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206611 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG
  ACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACAACCAAAGGATCAAGGCTGCTGTTCCAAGCAT
  CAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGCCACCTGGGCCGGCCTGATGGTGTT
  CCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCTGAACTCAAATCTCTGCTGGGCAAGGATGTTC
  TGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT
  CCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGAAACAAGGTT
  AAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCACTGTCCAAACTAGGAGATGTCTATGTCAATG
  ATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG
  ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT
  ATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA
  TGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT
  GTATGATGAAGAAGGAGCCAAGATTGTCAAAGATCTCATGTCCAAAGCTGAGAAAAATGGTGTGAAGATT
  ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCCAAGACTGGCCAAGCTACTGTGG
  CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGCAAGAAATATGCCGAGGC
  TGTGGGTCGAGCTAAGCAGATTGTTTGGAATGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG
  GGAACCAAGTCACTCATGGATGAGGTGGTGAAAGCCACTTCTAGGGGTTGCATCACTATCATAGGTGGTG
  GAGACACTGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG
  TGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206611 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGV
  PMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKV
  KAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLA
  ILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKI
  TLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFAR
  GTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC083355
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC083355.1
• RefSeq Size: 1727 bp
• RefSeq ORF: 1253 bp
• Locus ID: 18655
• MW: 44.6 kDa
• Gene Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]