Ap3m1 (BC024595) Mouse Tagged ORF Clone

CAT#: MR206629

Ap3m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, mu 1 subunit (cDNA clone MGC:30415 IMAGE:5026206)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap3m1
• Synonyms: 1200013D09Rik|C78982|R75378
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206629 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCACAGTCTATTTCTCATAAACTGCTCTGGCGACATTTTTCTAGAAAAACACTGGAAGAGCGTTG
  TAAGCCAGTCAGTGTGTGACTATTTCTTTGAAGCTCAGGAGAAAGCTGCTGATGTTGAAAATGTCCCACC
  TGTCATTTCAACACCTCACCACTACCTCATTAGTATCTACCGGGATAAGCTCTTCTTTGTGTCTGTGATA
  CAGACCGAAGTGCCACCTCTCTTTGTAATTGAATTTCTGCATCGAGTTGCTGACACTTTTCAGGACTACT
  TTGGTGAGTGTTCAGAGGCTGCAATTAAGGATAATGTGGTCATAGTATATGAGCTCTTGGAAGAAATGTT
  AGACAATGGATTCCCACTGGCTACTGAATCTAACATTTTGAAAGAACTGATTAAACCACCAACAATTCTA
  CGTTCTGTCGTCAATTCTATTACAGGCAGTAGTAATGTTGGGGATACACTCCCCACTGGGCAGCTATCCA
  ACATCCCATGGCGTCGAGCAGGAGTGAAGTACACAAACAATGAAGCCTATTTTGATGTAGTTGAAGAGAT
  AGATGCAATTATAGATAAATCAGGATCTACAGTCTTTGCAGAAATTCAAGGGGTCATTGATGCTTGCATT
  AAGCTGTCTGGAATGCCTGATCTCTCCCTCTCTTTCATGAACCCAAGGCTACTAGATGACGTCAGCTTCC
  ACCCATGCATCCGATTCAAACGCTGGGAGTCTGAAAGAGTTTTGTCATTCATTCCTCCAGATGGAAATTT
  CCGACTCATATCGTATCGTGTCAGCTCACAAAATCTAGTGGCAATACCAGTGTATGTGAAACACAGTATC
  AGCTTTAAGGAAAACAGCTCTTGTGGCCGGTTTGATATAACAATTGGACCGAAACAGAATATGGGAAAAA
  CAATTGAAGGAATCACAGTGACTGTTCACATGCCCAAAGTTGTGCTGAATATGAACCTGACACCAACGCA
  AGGCAGCTATACATTTGATCCCGTGACCAAGGTACTAGCATGGGATGTGGGCAAAATTACTCCACAAAAG
  CTCCCAAGTCTTAAAGGACTAGTAAATTTACAGTCAGGAGCACCCAAGCCAGAAGAGAACCCAAACCTCA
  ACATACAGTTCAAGATCCAGCAGCTTGCTATTTCAGGCTTAAAAGTGAACCGCTTGGACATGTATGGTGA
  GAAGTATAAGCCATTTAAAGGAGTCAAATATGTCACAAAGGCTGGGAAGTTCCAAGTGAGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206629 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVI
  QTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTIL
  RSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACI
  KLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSI
  SFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQK
  LPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024595
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024595, AAH24595
• RefSeq Size: 2157 bp
• RefSeq ORF: 1256 bp
• Locus ID: 55946
• MW: 46.9 kDa