Ap3m1 (BC024595) Mouse Tagged ORF Clone

CAT#: MR206629

  • TrueORF®

Ap3m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, mu 1 subunit (cDNA clone MGC:30415 IMAGE:5026206)


  "BC024595" in other vectors (3)


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Reconstitution Protocol

USD 250.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ap3m1
Synonyms 1200013D09Rik|C78982|R75378
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206629 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCACAGTCTATTTCTCATAAACTGCTCTGGCGACATTTTTCTAGAAAAACACTGGAAGAGCGTTG
TAAGCCAGTCAGTGTGTGACTATTTCTTTGAAGCTCAGGAGAAAGCTGCTGATGTTGAAAATGTCCCACC
TGTCATTTCAACACCTCACCACTACCTCATTAGTATCTACCGGGATAAGCTCTTCTTTGTGTCTGTGATA
CAGACCGAAGTGCCACCTCTCTTTGTAATTGAATTTCTGCATCGAGTTGCTGACACTTTTCAGGACTACT
TTGGTGAGTGTTCAGAGGCTGCAATTAAGGATAATGTGGTCATAGTATATGAGCTCTTGGAAGAAATGTT
AGACAATGGATTCCCACTGGCTACTGAATCTAACATTTTGAAAGAACTGATTAAACCACCAACAATTCTA
CGTTCTGTCGTCAATTCTATTACAGGCAGTAGTAATGTTGGGGATACACTCCCCACTGGGCAGCTATCCA
ACATCCCATGGCGTCGAGCAGGAGTGAAGTACACAAACAATGAAGCCTATTTTGATGTAGTTGAAGAGAT
AGATGCAATTATAGATAAATCAGGATCTACAGTCTTTGCAGAAATTCAAGGGGTCATTGATGCTTGCATT
AAGCTGTCTGGAATGCCTGATCTCTCCCTCTCTTTCATGAACCCAAGGCTACTAGATGACGTCAGCTTCC
ACCCATGCATCCGATTCAAACGCTGGGAGTCTGAAAGAGTTTTGTCATTCATTCCTCCAGATGGAAATTT
CCGACTCATATCGTATCGTGTCAGCTCACAAAATCTAGTGGCAATACCAGTGTATGTGAAACACAGTATC
AGCTTTAAGGAAAACAGCTCTTGTGGCCGGTTTGATATAACAATTGGACCGAAACAGAATATGGGAAAAA
CAATTGAAGGAATCACAGTGACTGTTCACATGCCCAAAGTTGTGCTGAATATGAACCTGACACCAACGCA
AGGCAGCTATACATTTGATCCCGTGACCAAGGTACTAGCATGGGATGTGGGCAAAATTACTCCACAAAAG
CTCCCAAGTCTTAAAGGACTAGTAAATTTACAGTCAGGAGCACCCAAGCCAGAAGAGAACCCAAACCTCA
ACATACAGTTCAAGATCCAGCAGCTTGCTATTTCAGGCTTAAAAGTGAACCGCTTGGACATGTATGGTGA
GAAGTATAAGCCATTTAAAGGAGTCAAATATGTCACAAAGGCTGGGAAGTTCCAAGTGAGGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206629 protein sequence
Red=Cloning site Green=Tags(s)

MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVI
QTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTIL
RSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACI
KLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSI
SFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQK
LPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC024595
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC024595, AAH24595
RefSeq Size 2157 bp
RefSeq ORF 1256 bp
Locus ID 55946
MW 46.9 kDa

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