Bzw1 (BC005466) Mouse Tagged ORF Clone

CAT#: MR206643

Bzw1 (Myc-DDK-tagged) - Mouse basic leucine zipper and W2 domains 1 (cDNA clone MGC:6434 IMAGE:3601190)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bzw1
• Synonyms: MGC6434, mKIAA0005
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206643 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAATCAAAAGCAGCAAAAACCAACGCTATCAGGCCAGCGTTTTAAAACCAGAAAAAGAGATGAAA
  AAGAGAGGTTTGACCCTACTCAGTTTCAAGACTGTATTATTCAAGGCTTAACTGAAACTGGTACTGATTT
  GGAAGCAGTAGCTAAGTTTCTTGATGCTTCTGGAGCAAAACTTGACTACCGCCGCTATGCAGAAACACTC
  TTTGACATTCTGGTGGCTGGCGGAATGCTGGCCCCGGGTGGTACACTGGCAGATGACATGATGCGCACAG
  ATGTCTGTGTGTTCGCAGCACAAGAAGACCTAGAGACCATGCAAGCATTCGCTCAGGTTTTTAACAAGCT
  AATCAGGCGCTACAAATACCTGGAGAAAGGTTTTGAAGATGAAGTTAAAAAGTTACTGCTCTTCCTAAAG
  GGGTTTTCAGAGTCGGAGAGGAACAAGCTTGCCATGTTGACTGGTGTCCTTCTGGCTAATGGAACCCTTA
  ATGCATCCATTCTTAACAGCCTTTATAATGAGAATTTGGTTAAAGAAGGGGTTTCAGCAGCTTTTGCTGT
  AAAGCTCTTTAAATCATGGATAAATGAAAAAGACATCAATGCAGTTGCTGCGAGTCTTCGGAAAGTCAGC
  ATGGACAACAGACTGATGGAGCTGTTCCCTGCCAATAAACAAAGTGTTGAGCATTTCACTAAGTATTTCA
  CTGAGGCGGGCTTGAAGGAGCTTTCAGAGTATGTCCGTAACCAGCAGACCATTGGGGCACGGAAGGAGCT
  CCAAAAGGAGCTGCAGGAGCAGATGTCCCGTGGGGACCCATTCAAGGACATAATTTTATATGTCAAGGAG
  GAGATGAAAAAAAATAACATTCCAGAACCAGTGGTCATTGGGATAGTCTGGTCCAGTGTGATGAGCACCG
  TGGAGTGGAACAAAAAAGAGGAGCTTGTAGCAGAGCAAGCCATCAAGCACTTGAAGCAATACAGCCCTCT
  CCTTGCTGCCTTCACTACTCAAGGTCAGTCTGAGCTGACCCTGCTACTGAAGATCCAGGAGTATTGCTAT
  GACAACATTCACTTCATGAAGGCCTTCCAGAAAATAGTGGTGCTTTTTTATAAAGCTGAGGTCCTGAGTG
  AAGAGCCCATTCTGAAGTGGTATAAAGACGCACATGTTGCAAAGGGCAAAAGTGTCTTCCTTGAGCAAAT
  GAAGAAGTTTGTAGAATGGCTCAAAAATGCTGAGGAGGAATCTGAGTCTGAAGCTGAAGAAGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206643 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNNQKQQKPTLSGQRFKTRKRDEKERFDPTQFQDCIIQGLTETGTDLEAVAKFLDASGAKLDYRRYAETL
  FDILVAGGMLAPGGTLADDMMRTDVCVFAAQEDLETMQAFAQVFNKLIRRYKYLEKGFEDEVKKLLLFLK
  GFSESERNKLAMLTGVLLANGTLNASILNSLYNENLVKEGVSAAFAVKLFKSWINEKDINAVAASLRKVS
  MDNRLMELFPANKQSVEHFTKYFTEAGLKELSEYVRNQQTIGARKELQKELQEQMSRGDPFKDIILYVKE
  EMKKNNIPEPVVIGIVWSSVMSTVEWNKKEELVAEQAIKHLKQYSPLLAAFTTQGQSELTLLLKIQEYCY
  DNIHFMKAFQKIVVLFYKAEVLSEEPILKWYKDAHVAKGKSVFLEQMKKFVEWLKNAEEESESEAEEGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005466
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005466, AAH05466
• RefSeq Size: 2840 bp
• RefSeq ORF: 1259 bp
• Locus ID: 66882
• MW: 48 kDa