3110043O21Rik (BC076612) Mouse Tagged ORF Clone

CAT#: MR206668

3110043O21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 3110043O21 gene (cDNA clone MGC:100148 IMAGE:30638238)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3110043O21Rik
• Synonyms: AI840585
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206668 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACTATCTGCCCCCCACCATCTCCTGCTGTTGCCAAGACAGAGATTGCTTTAAGTGGTGAATCAC
  CCTTGTTGGCGGCTACCTTTGCTTACTGGGATAATATTCTTGGTCCTAGAGTAAGGCATATTTGGGCTCC
  AAAGACAGACCAAGTGCTTCTCAGTGATGGAGAAATAACTTTTCTTGCCAACCACACTCTAAATGGAGAA
  ATTCTTCGAAATGCAGAGAGTGGGGCTATAGATGTAAAATTTTTTGTCTTATCTGAAAAAGGGGTAATTA
  TTGTTTCATTAATCTTCGACGGAAACTGGAATGGAGATCGGAGCACTTATGGACTATCAATTATACTGCC
  GCAGACAGAGCTGAGCTTCTACCTCCCACTTCACAGAGTGTGTGTTGACAGGCTAACACACATTATTCGA
  AAAGGAAGAATATGGATGCATAAGGAAAGACAAGAAAATGTCCAGAAAATTGTCTTGGAAGGCACAGAGA
  GGATGGAAGATCAGGGTCAGAGTATCATTCCCATGCTTACTGGGGAAGTCATTCCTGTAATGGAGCTGCT
  TGCATCTATGAAATCCCACAGTGTTCCTGAAGACATTGATATAGCTGATACAGTGCTCAATGATGATGAC
  ATTGGTGACAGCTGTCACGAAGGCTTTCTTCTCAATGCCATCAGCTCACACCTGCAGACCTGTGGCTGTT
  CCGTTGTAGTTGGCAGCAGTGCAGAGAAAGTAAATAAGATAGTAAGAACGCTGTGCCTTTTTCTGACACC
  AGCAGAGAGGAAATGCTCCAGGCTGTGTGAAGCAGAATCGTCCTTTAAGTACGAATCGGGACTCTTTGTG
  CAAGGCTTGCTAAAGGATGCAACAGGCAGTTTTGTCCTACCCTTCCGGCAAGTTATGTATGCCCCGTACC
  CCACCACGCACATTGATGTGGATGTCAACACTGTCAAGCAGATGCCACCGTGTCATGAACATATTTATAA
  TCAACGCAGATACATGAGGTCAGAGCTGACAGCCTTCTGGAGGGCAACTTCAGAAGAGGACATGGCGCAG
  GACACCATCATCTACACAGATGAGAGCTTCACTCCTGATTTGAATATTTTCCAAGATGTCTTACACAGAG
  ACACTCTAGTGAAAGCCTTCCTGGATCAGGTCTTCCATTTGAAGCCTGGCCTGTCTCTCAGGAGTACTTT
  CCTTGCACAGTTCCTCCTCATTCTTCACAGAAAAGCCTTGACACTAATCAAGTACATCGAGGATGATACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206668 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTICPPPSPAVAKTEIALSGESPLLAATFAYWDNILGPRVRHIWAPKTDQVLLSDGEITFLANHTLNGE
  ILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQTELSFYLPLHRVCVDRLTHIIR
  KGRIWMHKERQENVQKIVLEGTERMEDQGQSIIPMLTGEVIPVMELLASMKSHSVPEDIDIADTVLNDDD
  IGDSCHEGFLLNAISSHLQTCGCSVVVGSSAEKVNKIVRTLCLFLTPAERKCSRLCEAESSFKYESGLFV
  QGLLKDATGSFVLPFRQVMYAPYPTTHIDVDVNTVKQMPPCHEHIYNQRRYMRSELTAFWRATSEEDMAQ
  DTIIYTDESFTPDLNIFQDVLHRDTLVKAFLDQVFHLKPGLSLRSTFLAQFLLILHRKALTLIKYIEDDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC076612
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC076612, AAH76612
• RefSeq Size: 3432 bp
• RefSeq ORF: 1262 bp
• Locus ID: 73205
• MW: 47.3 kDa