Ap1m2 (NM_009678) Mouse Tagged ORF Clone

CAT#: MR206743

Ap1m2 (Myc-DDK-tagged) - Mouse adaptor protein complex AP-1, mu 2 subunit (Ap1m2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap1m2
• Synonyms: D9Ertd818e; mu1B; [m]1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206743 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCTCGGCAGTCTTCATTCTGGACGTCAAGGGCAAGCCTCTGATTAGCCGAAACTACAAAGGTG
  ATGTGCCCATGACAGAGATTGACCACTTCATGCCGCTGCTTATGCAGCGTGAGGAGGAGGGCGTGCTGGC
  CCCGCTGCTGAGCCACGGCCGAGTGCACTTCCTGTGGATCAAACACAGCAACCTCTACTTGGTGGCCACC
  ACGCTGAAGAACGCTAATGCCTCTCTGGTGTACTCCTTTCTGTACAAGACTGTGGAGGTCTTCTGCGAGT
  ACTTTAAGGAGCTGGAGGAGGAGAGCATCCGGGACAACTTCGTCATCGTGTACGAGCTGCTGGATGAGCT
  CATGGACTTCGGCTTTCCGCAGACCACAGACAGCAAGATCCTGCAGGAGTACATCACACAACAGGGCAAC
  AAGCTGGAGACTGGCAAATCTCGTGTACCACCTACAGTCACCAATGCGGTGTCGTGGCGCTCTGAGGGCA
  TTAAGTACAAAAAGAATGAGGTCTTCATTGATGTCATTGAGTCTGTCAACCTTCTGGTCAATGCTAATGG
  GAGTGTCTTGCTCAGCGAGATTGTGGGCACCATCAAGCTGAAGGTGTTTCTGTCTGGGATGCCAGAGCTG
  CGGCTGGGCCTCAATGACCGAGTGCTCTTTGAGCTTACTGGCCGCAGCAAGAACAAGTCTGTGGAACTGG
  AAGATGTGAAATTCCACCAATGTGTGAGGCTTTCACGCTTTGACAACGACCGAACCATCTCCTTCATACC
  CCCAGATGGGGACTTTGAACTCATGTCCTACCGGCTTAGCACCCAGGTCAAACCCCTGATCTGGATCGAG
  TCAGTCATTGAGAAATTCTCGCACAGCCGAGTGGAGATCATGGTGAAGGCCAAGGGTCAGTTTAAGAAGC
  AGTCGGTGGCCAATGGCGTGGAAATCTCTGTGCCCGTTCCTAGTGATGCAGACTCTCCGCGCTTCAAGAC
  CAGTGTGGGCAGTGCCAAGTATGTGCCTGAGAAGAATGTTGTCATCTGGAGCATCAAGTCATTCCCTGGG
  GGAAAGGAGTACTTGATGCGTGCCCACTTTGGCCTCCCCAGCGTGGAAACTGAGGAGGTAGAGGGCCGAC
  CACCCATCGGGGTCAAGTTTGAGATTCCATACTTTACCGTTTCTGGCATCCAGGTCCGTTACATGAAGAT
  CATCGAGAAAAGTGGGTACCAGGCCCTGCCCTGGGTCCGTTACATCACGCAGAGTGGTGATTATCAACTT
  CGTACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206743 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASAVFILDVKGKPLISRNYKGDVPMTEIDHFMPLLMQREEEGVLAPLLSHGRVHFLWIKHSNLYLVAT
  TLKNANASLVYSFLYKTVEVFCEYFKELEEESIRDNFVIVYELLDELMDFGFPQTTDSKILQEYITQQGN
  KLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLSGMPEL
  RLGLNDRVLFELTGRSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIWIE
  SVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPSDADSPRFKTSVGSAKYVPEKNVVIWSIKSFPG
  GKEYLMRAHFGLPSVETEEVEGRPPIGVKFEIPYFTVSGIQVRYMKIIEKSGYQALPWVRYITQSGDYQL
  RTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009678
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009678.1, NM_009678.2, NP_033808.1
• RefSeq Size: 1732 bp
• RefSeq ORF: 1272 bp
• Locus ID: 11768
• MW: 48.2 kDa
• Gene Summary: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. [UniProtKB/Swiss-Prot Function]