Kcnj8 (NM_008428) Mouse Tagged ORF Clone

CAT#: MR206756

Kcnj8 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 8 (Kcnj8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj8
• Synonyms: AI448900; gnite; Kir6.1; slmbr; sltr; uKATP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206756 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGCCAGGAAGAGCATCATCCCGGAGGAGTATGTGCTGGCGCGCATCGCAGCGGAGAACCTGCGCA
  AACCGCGCATCCGCGACCGTCTCCCCAAAGCCCGCTTCATCGCCAAGAGCGGAGCCTGCAACCTGGCACA
  CAAGAACATCCGAGAGCAAGGTCGCTTCCTGCAGGACATCTTCACCACCTTGGTAGACCTGAAGTGGCGT
  CACACGCTGGTCATCTTCACCATGTCCTTCCTCTGCAGCTGGCTGCTCTTTGCTATCATGTGGTGGCTGG
  TGGCCTTCGCCCACGGGGACATCTATGCTTACATGGAGAAAGGCACCATGGAGAAGAGTGGCCTGGAGTC
  CGCTGTCTGTGTGACCAATGTCAGGTCATTCACGTCTGCGTTTCTCTTCTCCATTGAGGTTCAAGTGACC
  ATTGGGTTCGGAGGGAGAATGATGACTGAGGAATGCCCTCTGGCCATCACGGTTTTGATTCTGCAGAACA
  TCGTGGGTCTGATCATCAACGCGGTCATGTTGGGTTGCATCTTCATGAAGACGGCGCAGGCCCACAGAAG
  GGCAGAGACGCTGATTTTCAGCCGCCATGCTGTGATTGCCGTCCGCAATGGCAAGCTGTGCTTCATGTTC
  CGGGTGGGTGACCTGAGGAAGAGCATGATCATTAGCGCCTCGGTGCGCATCCAGGTGGTCAAGAAAACCA
  CGACGCCAGAAGGGGAGGTGGTGCCTATTCATCAGCAGGACATTCCTGTTGATAATCCCATCGAGAGCAA
  TAATATCTTCCTAGTGGCCCCATTGATCATCTGCCACGTGATTGACAAGCGTAGCCCCCTGTATGATATC
  TCAGCAACTGACCTTGCCAATCAAGACCTGGAGGTCATAGTGATTCTCGAGGGCGTGGTAGAAACCACAG
  GCATCACCACACAAGCGCGGACCTCCTACATTGCGGAGGAGATCCAGTGGGGACACCGCTTCGTGTCAAT
  TGTGACTGAGGAGGAGGGCGTGTACTCTGTGGACTATTCCAAATTTGGTAACACGGTGAGAGTGGCTGCG
  CCAAGATGCAGTGCCCGGGAGCTGGATGAGAAGCCTTCCATCCTGATTCAGACCCTCCAAAAGAGTGAAC
  TGTCGCACCAGAATTCTCTGCGGAAGCGCAACTCCATGAGGAGAAACAACTCCATGAGGAGAAACAACTC
  CATCAGGAGGAATAACTCTTCCCTCATGGTGCCCAAGGTGCAGTTCATGACTCCAGAAGGAAACCAGTGT
  CCATCCGAATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206756 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLARKSIIPEEYVLARIAAENLRKPRIRDRLPKARFIAKSGACNLAHKNIREQGRFLQDIFTTLVDLKWR
  HTLVIFTMSFLCSWLLFAIMWWLVAFAHGDIYAYMEKGTMEKSGLESAVCVTNVRSFTSAFLFSIEVQVT
  IGFGGRMMTEECPLAITVLILQNIVGLIINAVMLGCIFMKTAQAHRRAETLIFSRHAVIAVRNGKLCFMF
  RVGDLRKSMIISASVRIQVVKKTTTPEGEVVPIHQQDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDI
  SATDLANQDLEVIVILEGVVETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVRVAA
  PRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRNNSIRRNNSSLMVPKVQFMTPEGNQC
  PSES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008428
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008428.1, NM_008428.2, NM_008428.3, NM_008428.4, NM_008428.5, NP_032454.1
• RefSeq Size: 2188 bp
• RefSeq ORF: 1275 bp
• Locus ID: 16523
• MW: 48 kDa