Acadl (NM_007381) Mouse Tagged ORF Clone

CAT#: MR206849

Acadl (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, long-chain (Acadl), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acadl
• Synonyms: AA960361; AU018452; C79855; LCAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206849 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCGCCTGCTCCTCCGCTCCCTGCGCGTCCTGAAAGCCCGCTCGGCGCCACGCCCGCCGCCCT
  CCGCCCGATGTTCTCATTCTGGAGCAGAAGCGCGTCTAGAAACTCCTTCTGCTAAAAAATTAACTGACGT
  TGGAATCAGGAGAATCTTTTCCTCGGAGCATGACATTTTCCGGGAGAGTGTAAGGAAGTTTTTCCAAGAA
  GAAGTGATTCCTCACCACACAGAATGGGAGAAAGCTGGAGAAGTGAGTAGAGAGGTCTGGGAAAAAGCTG
  GCAAGCAGGGCTTGCTTGGCATCAACATCGCAGAGAAACATGGCGGCATTGGTGGGGACTTGCTCTCAAC
  AGCAGTTACTTGGGAAGAGCAAGCGTACTCCAATTGCACAGGCCCTGGCTTCAGCCTCCACTCAGATATT
  GTCATGCCCTATATTGCGAATTACGGCACAAAAGAACAGATCGAGAAGTTCATCCCCCAGATGACGGCAG
  GCAAGTGTATCGGTGCCATAGCCATGACAGAGCCTGGGGCCGGAAGTGACTTACAAGGAGTAAGAACGAA
  CGCCAAAAGATCTGGGAGTGATTGGATTCTCAATGGAAGCAAGGTGTTCATCACTAATGGCTGGTTAAGT
  GATCTCGTGATCGTCGTGGCCGTCACCAACCGTGAAGCTCGATCGCCTGCCCATGGCATTAGCCTCTTTT
  TGGTGGAAAATGGAATGAAAGGATTTATCAAGGGCCGGAAGCTGCATAAGATGGGAATGAAAGCTCAGGA
  CACAGCAGAACTATTCTTTGAAGATGTCCGATTGCCAGCTAATGCCTTACTTGGAGAAGAGAATAAAGGC
  TTCTACTACCTCATGCAAGAGCTCCCACAGGAAAGGCTCTTAATTGCTGAGTTGGCGATTTCTGCCTGTG
  AGTTCATGTTTGAAGAAACCAGGAACTACGTGAAGCAAAGAAAAGCTTTTGGGAAAACAGTTGCACACAT
  ACAGACGGTGCAGCATAAACTAGCAGAATTAAAGACACACATATGTGTCACCAGAGCTTTTGTGGACAGC
  TGCCTGCAGTTGCATGAAACCAAACGTCTGGACTCCGGTTCCGCTTCCATGGCAAAATACTGGGCATCTG
  AATTACAAAACAGTGTAGCTTATGAATGTGTTCAACTCCACGGAGGCTGGGGGTACATGTGGGAGTACCC
  GATTGCAAAAGCTTATGTGGATGCTCGGGTGCAGCCGATCTACGGTGGTACCAACGAAATAATGAAAGAG
  CTGATCGCAAGACAGATCGTCAGTGACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206849 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAARLLLRSLRVLKARSAPRPPPSARCSHSGAEARLETPSAKKLTDVGIRRIFSSEHDIFRESVRKFFQE
  EVIPHHTEWEKAGEVSREVWEKAGKQGLLGINIAEKHGGIGGDLLSTAVTWEEQAYSNCTGPGFSLHSDI
  VMPYIANYGTKEQIEKFIPQMTAGKCIGAIAMTEPGAGSDLQGVRTNAKRSGSDWILNGSKVFITNGWLS
  DLVIVVAVTNREARSPAHGISLFLVENGMKGFIKGRKLHKMGMKAQDTAELFFEDVRLPANALLGEENKG
  FYYLMQELPQERLLIAELAISACEFMFEETRNYVKQRKAFGKTVAHIQTVQHKLAELKTHICVTRAFVDS
  CLQLHETKRLDSGSASMAKYWASELQNSVAYECVQLHGGWGYMWEYPIAKAYVDARVQPIYGGTNEIMKE
  LIARQIVSDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007381
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007381.2, NM_007381.3, NM_007381.4, NP_031407.2
• RefSeq Size: 1948
• RefSeq ORF: 1293
• Locus ID: 11363
• MW: 47.9 kDa
• Gene Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C12- and C16-acylCoA. In mice, deficiency of this gene can cause sudden death, cardiomyopathy as well as fasting and cold intolerance. [provided by RefSeq, Nov 2012]