Dcbld1 (BC026771) Mouse Tagged ORF Clone

CAT#: MR206887

Dcbld1 (Myc-DDK-tagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcbld1
• Synonyms: 4631413K11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206887 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCGGGGCTGGTGGGCCGAGTGTCCTGGCGCTGCTGTTCGCCGTGTGTGCTCCGCTCCGGTTGC
  AGGCGGAGGAGCTGGGTCCATATTGTGGGAGTTGGGCTGTTCCCAAAGAACTCCGGCTGAACTCAAACGA
  AGTGACTGTCCTCTTCAAGAGTGGATCTCACATTTCTGGCCGGGGCTTTCTGCTGACCTACGCCAGCAGT
  GACCATCCAGATTTAATAACCTGTTTGGAACGAGGCAGCCATTATTTCGAGGAAAAATACAGCAAATTCT
  GCCCAGCTGGCTGTAGAGACATAGCAGGAGATATTTCTGGGAATACAAAAGATGGCTACAGAGATACCTC
  TTTATTGTGCAAAGCTGCCATCCACGCAGGGATCATCACAGATGAACTAGGTGGCCACATCAACTTGCTT
  CAGAGCAAAGGGATAAGTCACTATGAAGGACTCCTGGCCAATGGCGTGCTCTCCCGGCATGGTTCTTTGT
  CGGAAAAGCGATTTCTTTTTACAACCCCAGGAATGAATATTACAACTGTGGCGATTCCATCAGTGATCTT
  CATCGCCCTCCTTCTGACTGGAATGGGGATCTTTGCAATCTGTAGAAAGAGGAAAAAGAAAGGAAATCCA
  TATGTGTCAGCTGACGCTCAGAAAACAGGCTGTTGGAAGCAGATTAAATATCCCTTTGCCAGGCATCAGT
  CGACGGAATTTACCATCAGCTATGACAATGAAAAAGAGATGACACAAAAGTTGGATCTCATCACTAGTGA
  TATGGCAGATTATCAGCAGCCTCTCATGATTGGCACAGGCACAGTCGCGAGAAAGGGCTCTACCTTCCGA
  CCCATGGACACAGACACTGAGGAGGTCAGAGTGAACACTGAGGCCAGCGGCCACTATGACTGTCCTCACC
  GCCCGGGCCGCCATGAGTACGCACTGCCTTTGACGCACTCAGAACCTGAGTATGCCACACCTATCGTGGA
  GCGGCACCTGCTGCGAGCTCACACCTTCTCCACACAGAGCGGCTACCGAGTCCCTGGGCCCAGGCCCACT
  CACAAACACTCCCATTCCTCTGGAGGCTTTCCTCCTGCTACAGGAGCCACCCAGATTGAAAGCTATCAGA
  GGCCAGCAAGCCCCAAGCCTGTGGGTGGTGGCTATGACAAGCCTGCTGCTAGCAGCTTCTTGGACAGCAG
  AGACCCAGCCTCTCAGTCACAGATGACTTCCGGGGGAGATGATGGTTATTCGGCACCCAGAACCGGTCTT
  GCGCCCCTCAACCAGACGGCCATGACTGCTCTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206887 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTGAGGPSVLALLFAVCAPLRLQAEELGPYCGSWAVPKELRLNSNEVTVLFKSGSHISGRGFLLTYASS
  DHPDLITCLERGSHYFEEKYSKFCPAGCRDIAGDISGNTKDGYRDTSLLCKAAIHAGIITDELGGHINLL
  QSKGISHYEGLLANGVLSRHGSLSEKRFLFTTPGMNITTVAIPSVIFIALLLTGMGIFAICRKRKKKGNP
  YVSADAQKTGCWKQIKYPFARHQSTEFTISYDNEKEMTQKLDLITSDMADYQQPLMIGTGTVARKGSTFR
  PMDTDTEEVRVNTEASGHYDCPHRPGRHEYALPLTHSEPEYATPIVERHLLRAHTFSTQSGYRVPGPRPT
  HKHSHSSGGFPPATGATQIESYQRPASPKPVGGGYDKPAASSFLDSRDPASQSQMTSGGDDGYSAPRTGL
  APLNQTAMTALL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026771
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026771, AAH26771
• RefSeq Size: 2819 bp
• RefSeq ORF: 1298 bp
• Locus ID: 66686
• MW: 46.8 kDa