Smyd2 (NM_026796) Mouse Tagged ORF Clone

CAT#: MR206911

Smyd2 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 2 (Smyd2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smyd2
• Synonyms: 1110020E07Rik; 4930402C15; KMT3C; Zmynd14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206911 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCGAGGCCCGCGGCGGCCTGGAGCGCTTCTGCAGTGCGGGCAAGGGCCGGGGGCTCCGCGCGC
  TGCGGCCCTTCCACGTGGGCGACCTCCTCTTCTCCTGCCCGGCCTACGCCTGCGTGCTCACCGTGGGCGA
  GCGGGGCCACCACTGCGAGTGCTGCTTCGCCAGGAAAGAAGGATTGTCAAAATGTGGACGGTGCAAGCAG
  GCGTTCTACTGCGACGTGGAATGTCAGAAGGAAGACTGGCCCCTGCACAAGCTGGAATGCTCCTCCATGG
  TTGTTTTGGGGGAGAACTGGAATCCTTCGGAGACTGTGCGGCTCACAGCAAGAATTCTGGCCAAACAGAA
  AATCCACCCAGAGAGGACACCTTCAGAGAAATTGTTGGCTGTGAGGGAGTTTGAGTCACATCTGGACAAG
  CTAGACAACGAGAAGAAAGATCTCATCCAGAGTGACATCGCCGCTCTCCACCAGTTCTACTCCAAGTACC
  TGGAATTCCCTGACCACAGCAGTCTCGTGGTGCTCTTCGCCCAGGTGAACTGTAATGGCTTCACTATTGA
  AGATGAAGAGCTCTCCCATTTGGGATCGGCGATATTCCCTGATGTTGCATTGATGAATCACAGCTGCTGC
  CCGAATGTCATTGTGACCTACAAAGGGACCCTGGCAGAAGTCAGAGCTGTACAGGAGATCCACCCAGGAG
  ACGAGGTGTTCACCAGCTACATCGACCTGCTATATCCAACAGAAGACAGGAACGACCGGCTAAGAGACTC
  CTATTTCTTTACCTGTGAGTGCCGGGAGTGTACAACCAAGGATAAGGACAAAGCCAAGGTGGAAGTCCGA
  AAGCTCAGCAGCCCGCCACAGGCAGAAGCCATCCGAGACATGGTCAGATACGCACGCAATGTCATCGAGG
  AGTTCCGGAGGGCCAAACACTACAAATCCCCTAGTGAGCTGCTGGAGATCTGCGAGCTTAGCCAGGAGAA
  GATGAGCTCCGTGTTTGAGGACAGCAATGTGTACATGCTGCACATGATGTACCAGGCCATGGGCGTCTGC
  CTGTACATGCAGGACTGGGAGGGAGCCCTGAAATACGGGCAGAAGATCATTAAGCCGTACAGTAAGCACT
  ACCCCGTGTACTCCCTCAACGTGGCTTCCATGTGGCTGAAGCTGGGAAGACTGTACATGGGTCTGGAAAA
  CAAAGCGGCCGGCGAGAAGGCCCTGAAGAAGGCCATTGCCATCATGGAGGTAGCTCACGGCAAGGACCAT
  CCGTACATCTCTGAAATCAAGCAGGAGATTGAGAGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206911 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRAEARGGLERFCSAGKGRGLRALRPFHVGDLLFSCPAYACVLTVGERGHHCECCFARKEGLSKCGRCKQ
  AFYCDVECQKEDWPLHKLECSSMVVLGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVREFESHLDK
  LDNEKKDLIQSDIAALHQFYSKYLEFPDHSSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCC
  PNVIVTYKGTLAEVRAVQEIHPGDEVFTSYIDLLYPTEDRNDRLRDSYFFTCECRECTTKDKDKAKVEVR
  KLSSPPQAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVC
  LYMQDWEGALKYGQKIIKPYSKHYPVYSLNVASMWLKLGRLYMGLENKAAGEKALKKAIAIMEVAHGKDH
  PYISEIKQEIESH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026796
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026796.1, NP_081072.1
• RefSeq Size: 1619 bp
• RefSeq ORF: 1302 bp
• Locus ID: 226830
• MW: 49.6 kDa