Crtac1 (BC024472) Mouse Tagged ORF Clone

CAT#: MR206937

Crtac1 (Myc-DDK-tagged) - Mouse cartilage acidic protein 1 (cDNA clone MGC:37356 IMAGE:4976509)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crtac1
• Synonyms: 2810454P21Rik|AW047536|Crtac1B|Lotus
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206937 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCTGAGGCCAGTGACCTGTCCCGGGGGATCCTGGCACTCAGGGACGTGGCGGCTGAGGCTGGGG
  TCAGCAAGTACACAGCGGGCCGGGGTGTCAGCGTAGGCCCCATTCTCAGCAGCAGTGCCTCAGATATCTT
  CTGTGACAACGAGAACGGGCCCAACTTCCTCTTCCACAACCAAGGCAACGGTACCTTTGTGGATACTGCG
  GCCAGTGCCGGCGTAGACGACCCTCATCAGCATGGCCGAGGTGTGGCCCTGGCAGATTTCAACCGTGACG
  GCAAAGTAGACATCGTCTATGGCAACTGGAACGGCCCACATCGCCTCTATCTACAGATGAGTGCTCACGG
  GAAGGTCCGGTTTCGGGACATTGCTTCGCCCAAGTTCTCCACGCCCTCCCCTGTCCGGACTGTCATTGCT
  GCCGACTTTGACAATGACCAGGAACTGGAAGTCTTCTTCAACAACATTGCCTACAGAAGCTCCTCAGCCA
  ATCGCCTCTTCCGTGTCATCCGCAGGGAGCACGGGGACCCTCTCATCGAGGAGCTCAATCCTGGTGACGC
  CCTAGAGCCTGAGGGCCGGGGTACAGGGGGCGTAGTGACCGACTTCGATGGCGATGGGATGCTGGATCTC
  ATCTTGTCACATGGAGAGTCCATGGCTCAGCCACTGTCTGTCTTCCGGGGAAATCAGGGCTTCAGCAACA
  ACTGGTTGCGTGTGGTACCTCGCACCCGGTTCGGGGCCTTTGCCAGGGGCGCCAAGGTTGTACTCTACAC
  CAAGAAGAGTGGGGCGCACCTACGGATCATTGATGGGGGTTCCGGCTACCTGTGTGAAATGGAGCCTGTG
  GCACATTTTGGCCTGGGACGGGATGAAGCCAGCAGTGTGGAGGTGACGTGGCCAGATGGCAAGATGGTGA
  GCCGAAGTGTGGCCAGCGAGGAGATGAACTCGGTGTTGGAGATCCTCTACCCCCAGGATGAGGACAAACT
  TCAGAACACAGCCCCACTAGAGTGCGGCCAAGGATTCTCCCAGCAGGACAATGGCCATTGCATGGACACC
  AATGAATGCATCCAGTTCCCATTTGTGTGCCCTCGAGACAAACCCGTATGTGTCAACACCTATGGAAGCT
  ACAGGTGCCGGACCAATAAAAGATGCAATCGGGGCTATGAACCCAATGAAGATGGCACAGCCTGTGTGGC
  TCAAGTGGCCTTTTTAGGTGGGTACTCATCGGCTGCCTTTAGACTCTCTGAGCCTCTCTCTCAGGCCTCG
  TATCTTTCTCTAGGCCTGGGACTTTGCCTTCAGTTATATGCACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206937 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPEASDLSRGILALRDVAAEAGVSKYTAGRGVSVGPILSSSASDIFCDNENGPNFLFHNQGNGTFVDTA
  ASAGVDDPHQHGRGVALADFNRDGKVDIVYGNWNGPHRLYLQMSAHGKVRFRDIASPKFSTPSPVRTVIA
  ADFDNDQELEVFFNNIAYRSSSANRLFRVIRREHGDPLIEELNPGDALEPEGRGTGGVVTDFDGDGMLDL
  ILSHGESMAQPLSVFRGNQGFSNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPV
  AHFGLGRDEASSVEVTWPDGKMVSRSVASEEMNSVLEILYPQDEDKLQNTAPLECGQGFSQQDNGHCMDT
  NECIQFPFVCPRDKPVCVNTYGSYRCRTNKRCNRGYEPNEDGTACVAQVAFLGGYSSAAFRLSEPLSQAS
  YLSLGLGLCLQLYAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024472
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024472, AAH24472
• RefSeq Size: 1558 bp
• RefSeq ORF: 1307 bp
• Locus ID: 72832
• MW: 47.3 kDa