Eef1g (BC083071) Mouse Tagged ORF Clone

CAT#: MR206964

Eef1g (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 1 gamma (cDNA clone MGC:103354 IMAGE:6329652)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef1g
• Synonyms: EF1G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206964 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGGCACCCTGTACACATACCCTGAAAATTGGAGGGCCTTCAAGGCCCTCATCGCTGCTCAGT
  ACAGCGGGGCTCAGGTCCGCGTGCTCTCCGCACCACCCCACTTCCACTTTGGCCAAACCAACCGCACCCC
  TGAATTTCTCCGAAAATTTCCTGCCGGCAAGGTTCCAGCATTTGAGGGTGATGATGGATTCTGTGTGTTT
  GAGAGCAATGCCATTGCCTATTATGTAAGCAATGAGGAGCTGCGAGGAAGTACGCCAGAGGCGGCAGCTC
  AGGTGGTGCAGTGGGTCAGCTTTGCTGACAGTGACATCGTTCCTCCAGCTAGTACCTGGGTGTTCCCCAC
  CTTGGGCATTATGCACCACAACAAACAGGCCACTGAAAATGCGAAAGAGGAGGTGAAGCGAATCCTGGGG
  CTGCTGGACACTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAG
  TTGTCTGTACCCTGTTGTGGCTCTATAAACAGGTCTTAGAGCCTTCTTTTCGCCAGGCCTTCCCCAATAC
  CAACCGGTGGTTCCTTACCTGCATTAACCAGCCCCAGTTCAGGGCTATCTTGGGGGAGGTGAAGCTGTGT
  GAGAAGATGGCTCAGTTTGATGCTAAGAAGTTTGCAGAGAGCCAGCCTAAAAAAGATACCCCAAGGAAAG
  AAAAGGGTTCACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCAGCTGCCCC
  AGCTCCTGAGGAAGAGATGGATGAGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTC
  GCTCACCTGCCCAAGAGTACCTTTGTGTTGGATGAGTTTAAGCGTAAGTACTCCAATGAGGACACCCTCT
  CTGTGGCACTGCCATATTTTTGGGAGCACTTTGATAAAGATGGCTGGTCCCTGTGGTATGCCGAGTACCG
  CTTCCCTGAAGAGCTGACCCAGACCTTCATGAGTTGCAACCTCATCACTGGGATGTTTCAGCGATTGGAC
  AAACTGAGGAAGAATGCCTTTGCTAGTGTTATCCTCTTTGGAACCAACAACAGCAGCTCCATTTCTGGTG
  TTTGGGTCTTCCGAGGCCAAGAGCTTGCCTTTCCGCTGAGTCCAGATTGGCAGGTGGACTATGAGTCGTA
  TACATGGCGGAAACTGGATCCTGGGAGCGAGGAAACCCAGACCCTGGTTCGAGAGTACTTTTCCTGGGAG
  GGGACCTTCCAGCATGTGGGCAAAGCCGTCAATCAAGGCAAGATCTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206964 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVF
  ESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVKRILG
  LLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAILGEVKLC
  EKMAQFDAKKFAESQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPF
  AHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYAEYRFPEELTQTFMSCNLITGMFQRLD
  KLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWE
  GTFQHVGKAVNQGKIFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC083071
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC083071, AAH83071
• RefSeq Size: 1460 bp
• RefSeq ORF: 1313 bp
• Locus ID: 67160
• MW: 50.1 kDa