Mef2c (BC026841) Mouse Tagged ORF Clone

CAT#: MR207045

  • TrueORF®

Mef2c (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2C (cDNA clone MGC:25468 IMAGE:4481227)


  "BC026841" in other vectors (4)


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Reconstitution Protocol

USD 270.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Mef2c
Synonyms 5430401D19Rik|9930028G15Rik|AV011172|Mef2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207045 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGAAAAAAGATTCAGATTACGAGGATAATGGATGAGCGTAACAGACAGGTGACTTTTACGAAGA
GGAAATTTGGATTGATGAAGAAGGCTTATGAGCTGAGCGTGCTGTGCGACTGTGAGATTGCACTGATCAT
CTTCAACAGCACCAACAAGCTGTTCCAGTACGCCAGCACTGACATGGATAAGGTGTTGCTCAAGTACACC
GAGTACAACGAGCCGCACGAGAGCCGGACAAACTCAGACATTGTGGAGACATTGAGAAAGAAGGGCCTCA
ATGGCTGTGACAGCCCAGATCCCGATGCAGACGATTCAGTAGGTCACAGCCCTGAGTCTGAGGACAAGTA
CAGGAAAATTAACGAAGATATTGATCTAATGATCAGCAGGCAAAGATTGTGTGCTGTTCCACCTCCCAGC
TTTGAGATGCCAGTTACCATCCCAGTGTCCAGCCATAACAGTTTGGTGTACAGCAATCCTGTCAGCACAC
TGGGAAACCCCAATCTTCTGCCACTGGCCCACCCGTCTCTGCAGAGGAATAGTATGTCTCCTGGTGTAAC
ACATAGACCTCCAAGTGCAGGTAACACAGGCGGTCTGATGGGCGGAGATCTGACATCCGGTGCAGGCACC
AGCGCAGGGAATGGATACGGCAACCCCCGGAACTCACCAGGCCTGCTGGTCTCACCTGGTAACCTGAACA
AGAATATACAAGCCAAATCTCCTCCCCCTATGAATCTAGGAATGAATAATCGTAAGCCAGATCTCCGCGT
TCTTATCCCACCTGGCAGCAAGAACACGATGCCATCAGTGTCTGAGGATGTGGATCTGCTGTTGAATCAA
AGGATAAATAACTCCCAGTCGGCTCAGTCATTGGCTACCCCGGTGGTTTCCGTAGCAACTCCTACTTTAC
CAGGACAAGGAATGGGAGGATATCCATCAGCCATTTCAACAACATATGGTACTGAGTACTCTCTGAGTAG
CGCAGATCTGTCATCTCTGTCTGGCTTCAACACTGCCAGTGCGCTCCACCTCGGCTCTGTAACTGGCTGG
CAGCAGCAGCACCTACATAACATGCCGCCATCTGCCCTCAGTCAGTTGGGAGACCGTACCACCACCCCTT
CGAGATACCCACAACACACCACGCGCCACGAGGCGGGGAGGTCTCCTGTTGACAGCTTGAGCAGCTGTAG
CAGTTCCTACGATGGGAGCGACCGAGAGGATCACCGGAACGAATTCCACTCCCCCATTGGACTCACCAGA
CCTTCGCCGGACGAAAGGGAAAGTCCTTCAGTCAAGCGCATGCGACTCTCTGAAGGATGGGCAACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207045 protein sequence
Red=Cloning site Green=Tags(s)

MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYT
EYNEPHESRTNSDIVETLRKKGLNGCDSPDPDADDSVGHSPESEDKYRKINEDIDLMISRQRLCAVPPPS
FEMPVTIPVSSHNSLVYSNPVSTLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGT
SAGNGYGNPRNSPGLLVSPGNLNKNIQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVSEDVDLLLNQ
RINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSVTGW
QQQHLHNMPPSALSQLGDRTTTPSRYPQHTTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPIGLTR
PSPDERESPSVKRMRLSEGWAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC026841
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC026841, AAH26841
RefSeq Size 3479 bp
RefSeq ORF 1328 bp
Locus ID 17260
MW 48 kDa

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