Slc18a2 (BC078449) Mouse Tagged ORF Clone

CAT#: MR207136

Slc18a2 (Myc-DDK-tagged) - Mouse solute carrier family 18 (vesicular monoamine), member 2 (cDNA clone MGC:90556 IMAGE:5705966)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc18a2
• Synonyms: Vmat2, 9330105E13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207136 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAGCGATCTGGTGCTGCTGCGATGGCTGCGGGACAGCCGCCACTCGCGCAAGCTGATCCTGT
  TCATCGTGTTCCTCGCGCTGCTGCTAGACAACATGCTGCTCACCGTCGTAGTTCCCATCATTCCCAGCTA
  CCTGTACAGCATTAAGCACGAGAAAAATACTACGGAAATCCAGACTGCCAGGCCAGCGCTCACAGCCTCC
  ACTTCCGAAAGCTTCCACAGCATCTTCTCTTACTATAACAACTCTACTGTGTTCACGGGGAATGCCACGG
  GGGGCCTCCCAGGAGGGGAGTCACCCAAGGCTACCACCACACAGCACACTGTGACCAACACGACTGTCCC
  TCCCGACTGCCCCAGTGAAGACAAAGACCTTCTAAATGAGAATGTGCAAGTTGGGCTGCTGTTTGCCTCC
  AAAGCCACTGTCCAGCTCCTCACCAACCCATTCATAGGACTTCTAACCAACAGAATTGGCTATCCAATTC
  CCATGTTTGCTGGATTCTGCATTATGTTTATCTCAACAGTTATGTTTGCCTTCTCCAGCAGCTATGCCTT
  CCTGCTGATCGCCAGGTCCCTGCAGGGAATCGGCTCCTCTTGCTCATCTGTGGCTGGGATGGGCATGCTG
  GCCAGCGTGTACACAGATGATGAAGAGAGGGGTAACGCCATGGGGATTGCTCTGGGGGGCCTGGCCATGG
  GGGTCTTAGTGGGACCCCCCTTTGGAAGTGTGCTCTATGAGTTTGTGGGGAAGACAGCTCCTTTCCTGGT
  GCTTGCTGCCTTGGTGCTCTTGGATGGAGAGTCAGAAGGGGACACCTCTTACGACCTTGCTGAAGGACCC
  ATACATTCTCATCGCTGCAGGCTCCATCTGCTTTGCAAACATGGGGATAGCCATGCTGGAGCCCGCCCTG
  CCCATCTGGATGATGGAGACCATGTGTTCCCGAAAGTGGCAGCTGGGCGTTGCCTTCCTGCCAGCGAGCA
  TCTCTTATCTCATTGGAACCAATATTTTTGGGATACTTGCACACAAAATGGGAAGGTGGCTGTGTGCTCT
  TCTGGGAATGATAGTTGTTGGGATCAGCATTTTATGTATTCCTTTTGCAAAAAATATCTACGGACTCATC
  GCTCCCAACTTTGGAGTTGGTTTTGCCATTGGGATGGTGGACTCCTCCATGATGCCTATCATGGGCTACC
  TGGTGGACCTGCGGCACGTGTCTGTCTATGGGAGTGTGTATGCCATTGCAGATGTAGCCTTCTGCATGGG
  GTATGCTATCGGTCCCTCTGCTGGTGGTGCTATTGCAAAGGCAATTGGCTTTCCTTGGCTCATGACAATT
  ATTGGGATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207136 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSDLVLLRWLRDSRHSRKLILFIVFLALLLDNMLLTVVVPIIPSYLYSIKHEKNTTEIQTARPALTAS
  TSESFHSIFSYYNNSTVFTGNATGGLPGGESPKATTTQHTVTNTTVPPDCPSEDKDLLNENVQVGLLFAS
  KATVQLLTNPFIGLLTNRIGYPIPMFAGFCIMFISTVMFAFSSSYAFLLIARSLQGIGSSCSSVAGMGML
  ASVYTDDEERGNAMGIALGGLAMGVLVGPPFGSVLYEFVGKTAPFLVLAALVLLDGESEGDTSYDLAEGP
  IHSHRCRLHLLCKHGDSHAGARPAHLDDGDHVFPKVAAGRCLPASEHLLSHWNQYFWDTCTQNGKVAVCS
  SGNDSCWDQHFMYSFCKKYLRTHRSQLWSWFCHWDGGLLHDAYHGLPGGPAARVCLWECVCHCRCSLLHG
  VCYRSLCWWCYCKGNWLSLAHDNYWDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC078449
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC078449, AAH78449
• RefSeq Size: 3702 bp
• RefSeq ORF: 1343 bp
• Locus ID: 214084
• MW: 49.3 kDa