Tnip2 (BC052083) Mouse Tagged ORF Clone

CAT#: MR207193

Tnip2 (Myc-DDK-tagged) - Mouse TNFAIP3 interacting protein 2 (cDNA clone MGC:62501 IMAGE:6404610)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnip2
• Synonyms: ABIN-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207193 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCTGGGGACCCAAGGTCTGGTAGACAGGACGGGGCCCCGCGTGCGGCCGCAGCGCTCTGTGGCC
  TGTACCACGAGGCCGGCCAGCAACTACAGCGCCTGAAGGATCAGCTGGCCGCGCGTGACGCCCTCATCGC
  GAGCCTCCGCACCCGCCTCGCGGCTCTGGAAGGGCACACGGCGCCGTCACTCGTGGACGCACTTCTGGAT
  CAGGTGGAGCGCTTCCGTGAGCAGCTGCGACGACAGGAGGAAGGCGCTTCGGAGACCCAGCTGCGGCAGG
  AAGTTGAAAGACTTACGGAGCGTCTAGAGGAAAAAGAGAGGGAGATGCAACAGCTGATGAGCCAGCCTCA
  GCATGAGCAAGAGAAGGAGGTAGTCTTGCTTCGGCGAAGTGTGGCAGAGAAGGAGAAAGCCAGGGCCGCC
  AGTGATGTTCTGTGCCGCTCCTTGGCTGATGAGACCCACCAACTGCGCAGGACATTGGCAGCCACTGCCC
  ACATGTGCCAACATCTGGCCAAATGTCTGGATGAACGACAGTGTGCACAGGGAGACGCTGGGGAGAAAAG
  CCCTGCTGAGGAGGTAAGATGGATCTGTGGCCAGGCCTACACCAAGGACCTGAAAAAGCCTCCTGGATGC
  TGGCTAGAGCAAACAAGCAGCGATGCTTCTGGCCAGAGTGTTATTAAGAAGTTACAGGAAGAAAATCGAC
  TGTTAAAACAGAAGGTGACTCATGTAGAAGACCTCAATGCTAAGTGGCAGCGTTATGATGCAAGTAGGGA
  CGAATATGTGAAGGGGTTGCATGCCCAGCTAAAGAGGCGGCAGGTCCCTCTGGAGCCTGAGCTGATGAAG
  AAGGAGATTTCCCGACTTAACAGACAGTTGGAGGAGAAAATAAGTGACTGTGCGGAAGCAAACCAGGAGC
  TGACAGCCATGAGGATGTCCCGGGACACTGCGCTGGAGCGAGTGCAGATGCTAGAACAGCAGATTCTTGC
  TTACAAGGATGACTTCAAATCAGAAAGGGCAGATCGGGAACGAGCGCACAGTAGGATTCAAGAGCTGGAG
  GAAAAGATCATGTCCTTGATGTACCAAGTGTCCCAGAGACAGGACTCCCGGGAGCCAGGACCCTGTCGGA
  TTCATACGGGGAACAAAACTGCCAAGTACTTAGAGATGGATGCACTGGAGCATGTGACCCCTGGCGGCTG
  GAGGCCTGAGTCTAGGTCCCAACAGATGGAACCTTCTGCAGAGGGTGGGCATGTGTGCACAGCCCAGAGA
  GGTCAGGGTGACCTTCAGTGCCCTCATTGCCTGCGGTGCTTCAGTGATGAGCAAGGCGAGGCATTCCTCA
  GGCACCTGTCTGAGTGCTGCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207193 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSGDPRSGRQDGAPRAAAALCGLYHEAGQQLQRLKDQLAARDALIASLRTRLAALEGHTAPSLVDALLD
  QVERFREQLRRQEEGASETQLRQEVERLTERLEEKEREMQQLMSQPQHEQEKEVVLLRRSVAEKEKARAA
  SDVLCRSLADETHQLRRTLAATAHMCQHLAKCLDERQCAQGDAGEKSPAEEVRWICGQAYTKDLKKPPGC
  WLEQTSSDASGQSVIKKLQEENRLLKQKVTHVEDLNAKWQRYDASRDEYVKGLHAQLKRRQVPLEPELMK
  KEISRLNRQLEEKISDCAEANQELTAMRMSRDTALERVQMLEQQILAYKDDFKSERADRERAHSRIQELE
  EKIMSLMYQVSQRQDSREPGPCRIHTGNKTAKYLEMDALEHVTPGGWRPESRSQQMEPSAEGGHVCTAQR
  GQGDLQCPHCLRCFSDEQGEAFLRHLSECCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052083
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052083, AAH52083
• RefSeq Size: 2039 bp
• RefSeq ORF: 1355 bp
• Locus ID: 231130
• MW: 51.6 kDa