Pgm2l1 (BC032150) Mouse Tagged ORF Clone

CAT#: MR207197

Pgm2l1 (Myc-DDK-tagged) - Mouse phosphoglucomutase 2-like 1 (cDNA clone MGC:29299 IMAGE:5004308)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pgm2l1
• Synonyms: BM32A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207197 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTACTGCATCTCATAACCGCAAAGAGGACAACGGGTACAAGGTTTATTGGGAAACTGGTGCTCAAA
  TCACATCTCCACATGATAAAGAAATTCTGAAATGTATAGAAGAGTGTGTGGAACCCTGGAATGATTCTTG
  GAATGATAATTTAGTGGACACCAGCCCACTGAAGAAAGATCCTCTGCAGGACATTTGCAAGAAATACATG
  GAAGATCTGAAGAAGATCTGTTTTTACAGGGACTTAAATTCAAAGACCACCTTGAAATTTGTTCATACCT
  CCTTCCATGGAGTTGGACATGACTACGTGCAGTTGGCTTTTCAAGTATTTGGTTTTAAGCCTCCAATTCC
  AGTCCCAGAACAGAAAGATCCAGATCCAGACTTTTCTACTGTTAAATGTCCAAACCCTGAAGAAGGAGAA
  TCTGTGCTGGAACTGTCCCTGAGACTGGCAGAGAAAGAAAATGCCCGGATAGTGCTAGCCACAGATCCTG
  ATGCCGACCGGCTGGCTGTGGCAGAACTTCAGGAGAATGGCCGTTGGAAAGTTTTCACAGGAAATGAGCT
  GGCAGCTTTGTTTGGGTGGTGGATGTTTGATTGCTGGAAGAAAAACAAACCAAACGCCGATGTGAAGAAT
  GTTTACATGCTAGCCACCACTGTCTCTTCTAAAATTTTAAAGGCAATTGCACTTAAAGAAGGATTTCATT
  TTGAAGAAACATTACCAGGCTTTAAATGGATTGGAAGTAGGATAAAAGACCTCCTAGGAAATGGAAAAGA
  AGTCCTTTTTGCATTTGAAGAGTCTATTGGCTTTCTGTGTGGAACCTCCGTGTTGGACAAAGATGGAGTG
  AGTGCAGCTGCTGTTGTTGCTGAGATGGCCTCTTTCCTGGATACCAGGAAGGTAACACTGATGGAGCAAC
  TGACAAAAGTGTATGAAATATACGGTTACCATATGTCCAAAACTTCATATTTCTTGTGTTATGATCCACC
  TACCATCAAAACCATATTTGAAAGGATTCGAAATTTTGAGTCTCCAAAGGAATATCCAAAATTTTGTGGG
  GCTTTTGCTATTTTGCATGTACGGGACATAACCACTGGATATGATAGCAGCCAGCCAAATAAGAAATCAG
  TACTGCCTGTGAGTAAAAACAGCCAAATGATTACATTTACCTTCCAAAACGGCTGTGTCGCTACTCTCCG
  AACAAGTGGGACAGAACCGAAGATAAAGTATTATGCAGAGATGTGTGCATCGCCTGGCCAGAGTGACACT
  ACCTTCCTGGAAGAAGAATTAAAGAAACTCATTGACGCGCTGATAGAGAATTTTCTTGAGCCCAGTAAGA
  ACGCACTGGTCTGGCGCTCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207197 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MITASHNRKEDNGYKVYWETGAQITSPHDKEILKCIEECVEPWNDSWNDNLVDTSPLKKDPLQDICKKYM
  EDLKKICFYRDLNSKTTLKFVHTSFHGVGHDYVQLAFQVFGFKPPIPVPEQKDPDPDFSTVKCPNPEEGE
  SVLELSLRLAEKENARIVLATDPDADRLAVAELQENGRWKVFTGNELAALFGWWMFDCWKKNKPNADVKN
  VYMLATTVSSKILKAIALKEGFHFEETLPGFKWIGSRIKDLLGNGKEVLFAFEESIGFLCGTSVLDKDGV
  SAAAVVAEMASFLDTRKVTLMEQLTKVYEIYGYHMSKTSYFLCYDPPTIKTIFERIRNFESPKEYPKFCG
  AFAILHVRDITTGYDSSQPNKKSVLPVSKNSQMITFTFQNGCVATLRTSGTEPKIKYYAEMCASPGQSDT
  TFLEEELKKLIDALIENFLEPSKNALVWRSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC032150
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032150, AAH32150
• RefSeq Size: 1927 bp
• RefSeq ORF: 1355 bp
• Locus ID: 70974
• MW: 51.2 kDa