Tuba1b (BC002219) Mouse Tagged ORF Clone

CAT#: MR207204

  • TrueORF®

Tuba1b (Myc-DDK-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568)


  "BC002219" in other vectors (3)


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Reconstitution Protocol

USD 270.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tuba1b
Synonyms Tuba2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207204 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCT
ACTGCCTGGAACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGA
CTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCAAGCATGTGCCCCGGGCAGTGTTCGTAGACCTG
GAACCCACGGTCATCGATGAAGTTCGCACTGGCACCTACCGCCAGCTCTTCCATCCTGAGCAGCTCATCA
CAGGCAAGGAGGATGCTGCCAATAACTATGCCCGTGGCCACTACACCATTGGCAAGGAGATCATTGACCT
TGTCCTGGACAGGATTCGCAAGCTGGCTGACCAGTGCACGGGTCTCCAGGGCTTCTTGGTTTTCCACAGC
TTTGGCGGGGGAACTGGCTCTGGCTTCACCTCCCTGCTGATGGAGCGGCTCTCTGTGGATTACGGAAAGA
AGTCCAAGCTGGAGTTCTCCATTTACCCAGCCCCCCAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTC
CATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT
GACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGA
TTGTGTCTTCCATCACTGCTTCCCTCAGATTTGATGGGGCCCTGAATGTTGATCTGACAGAATTCCAGAC
CAACCTGGTACCCTACCCTCGCATCCACTTCCCTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAA
GCCTACCATGAGCAGCTTTCTGTAGCAGAGATCACCAATGCCTGCTTTGAGCCAGCCAACCAGATGGTGA
AATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTATACCGTGGTGATGTGGTTCCCAAAGA
TGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGCAGCATCCAGTTTGTAGACTGGTGCCCCACTGGC
TTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGGTGCAGAGAG
CTGTGTGCATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTAGATCACAAGTTTGATCT
GATGTATGCCAAGCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCTGAG
GCCCGTGAGGACATGGCTGCCCTAGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTGGAAGGCGAGG
GGGAGGAAGAAGGAGAGGAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207204 protein sequence
Red=Cloning site Green=Tags(s)

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC002219
ORF Size 1353 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC002219, AAH02219
RefSeq Size 1616 bp
RefSeq ORF 1355 bp
Locus ID 22143
MW 50.2 kDa
Gene Summary Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]

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