Tuba1b (BC002219) Mouse Tagged ORF Clone

CAT#: MR207204

Tuba1b (Myc-DDK-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tuba1b
• Synonyms: Tuba2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207204 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCT
  ACTGCCTGGAACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGA
  CTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCAAGCATGTGCCCCGGGCAGTGTTCGTAGACCTG
  GAACCCACGGTCATCGATGAAGTTCGCACTGGCACCTACCGCCAGCTCTTCCATCCTGAGCAGCTCATCA
  CAGGCAAGGAGGATGCTGCCAATAACTATGCCCGTGGCCACTACACCATTGGCAAGGAGATCATTGACCT
  TGTCCTGGACAGGATTCGCAAGCTGGCTGACCAGTGCACGGGTCTCCAGGGCTTCTTGGTTTTCCACAGC
  TTTGGCGGGGGAACTGGCTCTGGCTTCACCTCCCTGCTGATGGAGCGGCTCTCTGTGGATTACGGAAAGA
  AGTCCAAGCTGGAGTTCTCCATTTACCCAGCCCCCCAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTC
  CATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT
  GACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGA
  TTGTGTCTTCCATCACTGCTTCCCTCAGATTTGATGGGGCCCTGAATGTTGATCTGACAGAATTCCAGAC
  CAACCTGGTACCCTACCCTCGCATCCACTTCCCTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAA
  GCCTACCATGAGCAGCTTTCTGTAGCAGAGATCACCAATGCCTGCTTTGAGCCAGCCAACCAGATGGTGA
  AATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTATACCGTGGTGATGTGGTTCCCAAAGA
  TGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGCAGCATCCAGTTTGTAGACTGGTGCCCCACTGGC
  TTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGGTGCAGAGAG
  CTGTGTGCATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTAGATCACAAGTTTGATCT
  GATGTATGCCAAGCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCTGAG
  GCCCGTGAGGACATGGCTGCCCTAGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTGGAAGGCGAGG
  GGGAGGAAGAAGGAGAGGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207204 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
  EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
  FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
  DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
  AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTG
  FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
  AREDMAALEKDYEEVGVDSVEGEGEEEGEEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002219
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002219, AAH02219
• RefSeq Size: 1616 bp
• RefSeq ORF: 1355 bp
• Locus ID: 22143
• MW: 50.2 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]