Dnm2 (BC010233) Mouse Tagged ORF Clone

CAT#: MR207259

Dnm2 (Myc-DDK-tagged) - Mouse dynamin 2 (cDNA clone MGC:7779 IMAGE:3499181)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm2
• Synonyms: Dyn2|Udnm|b2b2159Clo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207259 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTTGAAGCCATCGTGAAAAAACAGCTTGTAAAACTCAAAGAGCCGAGTTTGAAGTGTGTTGACC
  TGGTGGTGTCGGAGCTAGCCACGGTCATTAAAAAGTGTGCTGAGAAGCTGAGTTCCTACCCCCGGCTGCG
  AGAGGAGACCGAGCGAATTGTCACCACCTACATCAGGGAGCGAGAAGGGAGAACCAAGGACCAGATTCTT
  CTTCTGATTGACATTGAGCAGTCGTACATCAACACAAACCATGAAGACTTCATTGGATTTGCCAATGCCC
  AGCAGAGGAGCACGCAGCTGAACAAGAAGAGGGCCATACCCAATCAGGGGGAGATCTTGGTGATCCGCAG
  GGGCTGGTTGACCATCAACAATATCAGCTTGATGAAGGGTGGCTCCAAGGAGTACTGGTTCGTGCTGACA
  GCTGAGTCATTGTCTTGGTACAAGGATGAGGAGGAGAAAGAAAAGAAGTACATGCTGCCACTGGACAACC
  TCAAGATCCGGGACGTGGAAAAGGGCTTCATGTCCAACAAGCACGTGTTTGCCATCTTCAACACAGAGCA
  GAGGAATGTCTACAAGGACCTTCGACAGATTGAACTGGCTTGTGACTCCCAGGAAGATGTGGACAGCTGG
  AAGGCTTCGTTCCTGCGCGCTGGGGTCTACCCAGAGAAGGACCAGGCAGAGAATGAGGATGGAGCACAAG
  AGAACACCTTCTCCATGGACCCGCAGCTGGAGCGACAGGTGGAGACCATCCGTAACCTGGTGGACTCCTA
  CGTGGCCATCATCAACAAGTCCATCCGTGACCTTATGCCAAAGACCATCATGCACCTCATGATCAACAAC
  ACAAAGGCCTTCATCCACCATGAGCTGCTGGCTTACCTGTATTCATCAGCAGACCAGAGCAGCCTCATGG
  AAGAGTCAGCCGAGCAGGCTCAGCGGCGGGACGACATGCTGCGCATGTACCACGCACTGAAGGAGGCCCT
  CAACATTATTGGGGACATCAGCACCAGCACCGTGTCCACACCCGTGCCCCCACCGGTCGATGACACGTGG
  CTCCAAAACACCAGCGGCCACAGCCCCACTCCACAGCGCCGACCTGTGTCCAGTGTGCACCCACCAGGCC
  GGCCACCTGCAGTGCGGGGTCCTACACCGGGGCCTCCCCTGATTCCTATGCCTGTGGGGGCCACATCCTC
  CTTCTCAGCACCTCCCATCCCATCCCGGCCTGGACCACAGAGTGTGTTTGCCAACAACGACCCCTTCTCT
  GCGCCACCTCAGATACCATCTCGGCCAGCACGGATTCCCCCTGGTATCCCTCCAGGAGTGCCCAGGCGAC
  CACCTCCATTGGCTCCTGCCCGACCTTTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207259 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFEAIVKKQLVKLKEPSLKCVDLVVSELATVIKKCAEKLSSYPRLREETERIVTTYIREREGRTKDQIL
  LLIDIEQSYINTNHEDFIGFANAQQRSTQLNKKRAIPNQGEILVIRRGWLTINNISLMKGGSKEYWFVLT
  AESLSWYKDEEEKEKKYMLPLDNLKIRDVEKGFMSNKHVFAIFNTEQRNVYKDLRQIELACDSQEDVDSW
  KASFLRAGVYPEKDQAENEDGAQENTFSMDPQLERQVETIRNLVDSYVAIINKSIRDLMPKTIMHLMINN
  TKAFIHHELLAYLYSSADQSSLMEESAEQAQRRDDMLRMYHALKEALNIIGDISTSTVSTPVPPPVDDTW
  LQNTSGHSPTPQRRPVSSVHPPGRPPAVRGPTPGPPLIPMPVGATSSFSAPPIPSRPGPQSVFANNDPFS
  APPQIPSRPARIPPGIPPGVPRRPPPLAPARPFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010233
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010233, AAH10233
• RefSeq Size: 1875 bp
• RefSeq ORF: 1364 bp
• Locus ID: 13430
• MW: 51.3 kDa