C1rl (BC070472) Mouse Tagged ORF Clone

CAT#: MR207294

C1rl (Myc-DDK-tagged) - Mouse complement component 1, r subcomponent-like (cDNA clone MGC:92956 IMAGE:30627947)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1rl
• Synonyms: C1r-LP, C1rlp, C1rl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207294 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGGTGGCTCCTTTGGGGAATCCTCCATACTTGCCCCACACAGGCCTCTGTGCTTTTGGCCCAGC
  AGTCACCACAGCAACTGACATCCCCTGGATACCCAGAGCCATATCTCAAAGGCCAGGAGAGCCACACTGA
  CATCGAGGCTCCAGAGGGGTTTGCTGTGAGGCTGACCTTCCAGGACTTTGATCTGGAGCCATCCCCAGAC
  TGTGAAGGCGACTCTGTCACAATTTCAACTGGAGGAACAGATGCAACCCGGCTGTGTGGGCGGCAAGGTT
  CCCCTCTGGGGAACCCTCCTGGTCACAGGGAGTTTGTATCTTCTGGTAGGAGCCTGCGGCTGACCTTCCA
  GGCACACTCGTCCTCCAAGAGCAAAATCACACACCTCCACAAAGGTTTCCTGGCTCTCTACCAAGCTGTA
  GCTGTGAACCAACCCAATGGGGACACCGAGGCTGTCACCACACCCGGAGCTCCCAAGATTCAGAACCACT
  GCCAAGATCCTTATTATAAGGCAGACCAGACAGGGACACTTAGCTGCCCATCCTCGTGGAAGTGGAAGGA
  CAGACAGGATGGAGGAGAGGTTCCTGAATGCGTGCCAGTCTGTGGAAGACCAGTCGTCCCCCTTGCTGAG
  AACCCAAACACTTTTGGTTCTTCCCGAGCTAAACTGGGCAACTTCCCCTGGCAAGCCTTCACCAGCATCT
  ATGGTCGTGGGGGTGGAGCCCTGCTGGGTGACAGATGGATCCTGACGGCTGCCCATACCATCTACCCCAA
  GGATAGCATCTATCTCAGAAGGAACCAGAATGTGGAAGTGTTTCTGGGTCACACAGACATAGACGAGCTA
  CTGAAACTCGGGAACCATCCTGTCCGTAGGGTTGTGGTTCACCCAGACTACCGCCAACATGAGTCCCACA
  ACTTCAATGGAGATATTGCTCTCCTGGAACTTGAGCAGAGAGTCCCTCTGGGGCCCAATCTCCTCCCAGT
  CTGTCTGCCGGACAACGAGACCCTCTACCACAGCGGCCTGTGGGGTTACGTCAGTGGGTTTGGTGTGGAG
  ATGGGCTGGTTAACAACAAAGTTGAAATACTCAAAACTGCCTGTAGCTCCCAGGGAGGCCTGTGAAGCCT
  GGCTCCACCAGAGGCAGCGAACTGAGGTGTTTTCTGACAATATGTTCTGTGTTGGGGAAGAGATGCAGGT
  GAACAGTGTCTGCCAGGGAGACAGCGGCAGCGTCTATGTGGTGTGGGACGATCTTGCTCTCCGCTGGGTG
  GCCACAGGCATTGTATCCTGGGGCATCGGGTGTGGCAAGGGGTACGGCTTCTACACCAAAGTGCTCAGCT
  ACATGGATTGGATTAAGAGGGTGATTGAAGGTAAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207294 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCWWLLWGILHTCPTQASVLLAQQSPQQLTSPGYPEPYLKGQESHTDIEAPEGFAVRLTFQDFDLEPSPD
  CEGDSVTISTGGTDATRLCGRQGSPLGNPPGHREFVSSGRSLRLTFQAHSSSKSKITHLHKGFLALYQAV
  AVNQPNGDTEAVTTPGAPKIQNHCQDPYYKADQTGTLSCPSSWKWKDRQDGGEVPECVPVCGRPVVPLAE
  NPNTFGSSRAKLGNFPWQAFTSIYGRGGGALLGDRWILTAAHTIYPKDSIYLRRNQNVEVFLGHTDIDEL
  LKLGNHPVRRVVVHPDYRQHESHNFNGDIALLELEQRVPLGPNLLPVCLPDNETLYHSGLWGYVSGFGVE
  MGWLTTKLKYSKLPVAPREACEAWLHQRQRTEVFSDNMFCVGEEMQVNSVCQGDSGSVYVVWDDLALRWV
  ATGIVSWGIGCGKGYGFYTKVLSYMDWIKRVIEGKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC070472
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC070472, AAH70472
• RefSeq Size: 1726 bp
• RefSeq ORF: 1370 bp
• Locus ID: 232371
• MW: 50.6 kDa