Cep55 (NM_028293) Mouse Tagged ORF Clone

CAT#: MR207352

Cep55 (Myc-DDK-tagged) - Mouse centrosomal protein 55 (Cep55), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cep55
• Synonyms: 1200008O12Rik; 2700032M20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207352 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCAAGAAGTCCCAAAGATTTAATTAAAAGCAAATGGGGATCAAGGCCTAGTAGCTCCAAGTCAG
  ACACTGCATTAGAGAAATTTAAGGGAGAAATTGCAGCCTTTAAAACATCCCTGGATGAAATCACCTCTGG
  GAAAGGAAAAATGGCCGAAAAAGACAGAAGCAGACTTTTGGAGAAAATCCAAGTTCTTGAAGCCGAGAGA
  GAAAAGAATGTTTATTACCTCCTGGAGAAGGACAAAGAAATACAGAGACTCAAAGACCACCTAAGGTCAA
  GATATAGCTCTTCTTCATTGTTCGAGCAGCTGGAAGAGAAAACAAAGGAATGTGAGAAGAAGCAGCAGTT
  GCTGGAGTCCTTATCCAAAGAGACAGATGTCCTGAAAAACCAGCTGTCCGCCACGACGAAAAGACTTTCT
  GAACTTGAAAGCAAAGCCAGTACACTCCACCTGTCACAGAGCATGCCTGCAAACTGCTTCAACTCCTCAA
  TGAATAGTATTCATGAAAAGGAGATGCAGCTGAAAGATGCTCTGGAGAAAAACCAGCAATGGCTTGTGTA
  TGATCAGCAGCGAGAGGCCTACGTTAAAGGGCTTTTAGCAAAGATCTTCGAGCTGGAAAAGAGAACAGAG
  ACAGCCGCTGCTTCCCTCACACAGCAGATGAAGAAGATTGAATCAGAAGGTTATCTTCAAGTGGAGAAGC
  AAAAATATGATCATCTCTTGGAAAATGCCAAAAAAGATCTCGAAGTTGAGCGACAAGCCGTGACTCAGTT
  GCGTTTAGAACTCGATGAATTTCGAAGGAAATACGAAGAAGCTCGAAAAGAAGTTGAAGACTTAAATCAG
  CTGTTGAGCTCTCAGCGAAAGGCAGACATCCAGCACCTGGAGGAGGACAAGCAGAAGACGGAGAGGATCC
  AAAAGCTGAGGGAAGAGAGCAGTATCTTCAAGGGAAAGCTGGAAGAGGAGAGGAAGAGGTCGGAGGAGCT
  CTTATCCCAGGTCCGAATTCTCTACGATTCTCTGTTAAAGCACCAAGAGGAGCAGGCCAGGGTAGCTCTA
  CTGGAACAGCAGATGCAGGCATGTACTCTAGACTTTGAAAATGAAAAACTCGACCGCCAGAATATGCAGC
  ATCAACTCTATGTGATTCTTAAGGAACTCCGAAAAGCAAAAAGTCAAATAACACAGTTGGAATCCTTGCA
  GCTACATGGGTTTACCATCACAGAGCAGCCATTCCCACTCCAGAGAGAACCTGAAAGCAGAGTGAAAGCT
  ACCTCACCCAAAAGTCCCAGTGCTGCACTCAATGACAGCCTAGTGGAATGTCCCAAGTGCAGCGTACAGT
  ACCCAGCCACCGAGCATCGAGACCTGCTTGTCCATGTCGAATACTGCATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207352 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSRSPKDLIKSKWGSRPSSSKSDTALEKFKGEIAAFKTSLDEITSGKGKMAEKDRSRLLEKIQVLEAER
  EKNVYYLLEKDKEIQRLKDHLRSRYSSSSLFEQLEEKTKECEKKQQLLESLSKETDVLKNQLSATTKRLS
  ELESKASTLHLSQSMPANCFNSSMNSIHEKEMQLKDALEKNQQWLVYDQQREAYVKGLLAKIFELEKRTE
  TAAASLTQQMKKIESEGYLQVEKQKYDHLLENAKKDLEVERQAVTQLRLELDEFRRKYEEARKEVEDLNQ
  LLSSQRKADIQHLEEDKQKTERIQKLREESSIFKGKLEEERKRSEELLSQVRILYDSLLKHQEEQARVAL
  LEQQMQACTLDFENEKLDRQNMQHQLYVILKELRKAKSQITQLESLQLHGFTITEQPFPLQREPESRVKA
  TSPKSPSAALNDSLVECPKCSVQYPATEHRDLLVHVEYCMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028293
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028293.1, NP_082569.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 1389 bp
• Locus ID: 74107
• MW: 53.9 kDa