Asl (NM_133768) Mouse Tagged ORF Clone

CAT#: MR207413

Asl (Myc-DDK-tagged) - Mouse argininosuccinate lyase (Asl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asl
• Synonyms: 2510006M18Rik; ASAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207413 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAGAGAGCGGGAAGCTATGGGGTGGCCGGTTTGTGGGTGCTGTCGACCCCATCATGGAGAAGT
  TCAACTCATCTATCTCCTATGACCGGCATCTGTGGAATGTGGATGTGCAGGGGAGCAAGGCCTACAGCAG
  GGGCCTGGAGAAGGCGGGGCTTCTCACCAAAGCCGAGATGCAGCAGATACTGCAAGGGTTGGACAAGGTT
  GCTGAAGAGTGGGCCCAAGGCACCTTCAAATTACATCCTAATGATGAAGACATCCACACAGCCAACGAGC
  GGCGCCTGAAGGAACTCATCGGTGAAGCTGCAGGGAAGCTACACACAGGACGAAGTCGCAACGACCAGGT
  GGTCACGGACCTCAGGCTGTGGATGAGGCAGACCTGCTCAAAACTCTCCGCCCTCCTCCGGGTGCTCATT
  GGAACCATGGTCGACCGGGCAGAGGCGGAACGTGATGTCCTCTTCCCAGGGTACACACACTTGCAGAGAG
  CTCAGCCCATTCGCTGGAGCCACTGGATCCTGAGCCACGCTGTTGCGCTGACACGAGATTCAGAGAGACT
  CTTGGAGGTGCAGAAGCGGATCAATGTCTTGCCACTGGGGAGTGGGGCCATTGCAGGCAACCCTCTGGGT
  GTGGACCGGGAGCTACTCCGTGCAGAACTGAACTTTGGGGCCATCACTCTCAACAGCATGGATGCCACCA
  GTGAGAGAGACTTTGTGGCTGAGTTCCTGTTTTGGGCTTCTCTGTGTATGACCCACCTCAGCAGGATGGC
  AGAGGACCTAATTCTCTATGGTACCAAGGAATTCAGCTTTGTGCAACTCTCAGATGCCTACAGCACCGGA
  AGCAGCTTGATGCCCCAGAAGAAAAACCCAGACAGCCTGGAGCTGATCCGGAGCAAGGCGGGCCGAGTGT
  TTGGACGGTGTGCCGGACTCCTGATGACCCTCAAGGGACTTCCAAGCACCTACAACAAGGACTTACAGGA
  AGACAAGGAGGCTGTGTTTGAAGTGTCTGACACCATGATAGCTGTCCTCCAAGTGGCCACTGGAGTCATC
  TCTACATTGCAGATTCATCGTGAGAACATGAAACAGGCGCTCAGCCCTGACATGCTGGCCACGGACCTCG
  CCTACTACCTGGTCCGCAAAGGGATGCCATTCCGCCAGGCCCACGAGGCCTCAGGGAAAGCTGTGTTCAT
  GGCAGAGACTAAAGGAGTGGCTCTCAACCTGCTGTCGCTACAGGAGCTGCAGACCATCAGTCCCCTGTTC
  TCAGGTGATGTGAGTCACGTGTGGGACTACAGTCACAGCGTGGAACAGTACAGTGCCTTGGGTGGCACAG
  CGAAATCCAGTGTCGAATGGCAGATCAGGCAGGTGCGGGCCCTACTGCAAGCCCAGGAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207413 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASESGKLWGGRFVGAVDPIMEKFNSSISYDRHLWNVDVQGSKAYSRGLEKAGLLTKAEMQQILQGLDKV
  AEEWAQGTFKLHPNDEDIHTANERRLKELIGEAAGKLHTGRSRNDQVVTDLRLWMRQTCSKLSALLRVLI
  GTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVQKRINVLPLGSGAIAGNPLG
  VDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYGTKEFSFVQLSDAYSTG
  SSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMIAVLQVATGVI
  STLQIHRENMKQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNLLSLQELQTISPLF
  SGDVSHVWDYSHSVEQYSALGGTAKSSVEWQIRQVRALLQAQEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133768
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133768.1, NM_133768.2, NM_133768.3, NM_133768.4, NM_133768.5, NP_598529.1
• RefSeq Size: 1589 bp
• RefSeq ORF: 1395 bp
• Locus ID: 109900
• MW: 51.7 kDa