Vim (NM_011701) Mouse Tagged ORF Clone

CAT#: MR207446

Vim (Myc-DDK-tagged) - Mouse vimentin (Vim)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vim
• Synonyms: MGC102095
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207446 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACCAGGTCTGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGTGGCTCCGGCACATCGAGCCGGC
  CCAGCTCCAACCGGAGCTATGTGACCACGTCCACACGCACCTACAGTCTGGGCAGCGCACTGCGCCCCAG
  CACTAGCCGCAGCCTCTATTCCTCATCCCCCGGTGGCGCCTATGTGACCCGGTCCTCGGCAGTGCGCCTG
  CGGAGCAGCGTGCCGGGCGTGCGGCTGCTTCAAGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACA
  CTGAGTTCAAGAACACCCGCACCAACGAGAAGGTAGAACTGCAGGAGCTGAATGACCGCTTTGCCAACTA
  CATCGACAAGGTGCGCTTCCTCGAGCAGCAGAACAAAATCCTGCTGGCTGAGCTCGAGCAGCTCAAGGGC
  CAGGGCAAGTCGCGCCTGGGCGACCTGTACGAGGAGGAGATGCGGGAGCTGCGCCGGCAGGTGGATCAGC
  TCACCAACGACAAGGCCCGTGTCGAGGTGGAGCGGGACAACCTGGCCGAGGACATCATGCGGCTGCGAGA
  GAAATTGCAGGAGGAGATGCTCCAGAGAGAGGAAGCCGAAAGCACCCTGCAGTCATTCAGACAGGATGTT
  GACAATGCTTCTCTGGCACGTCTTGACCTTGAACGGAAAGTGGAATCCTTGCAGGAAGAAATTGCCTTTT
  TGAAGAAACTGCACGATGAAGAGATCCAGGAGCTGCAGGCCCAGATTCAGGAACAGCATGTCCAGATCGA
  TGTGGACGTTTCCAAGCCTGACCTCACTGCTGCCCTGCGTGATGTGCGCCAGCAGTATGAAAGCGTGGCT
  GCCAAGAACCTCCAGGAGGCCGAGGAATGGTACAAGTCCAAGTTTGCTGACCTCTCTGAGGCTGCCAACC
  GGAACAACGATGCCCTGCGCCAGGCCAAGCAGGAGTCAAACGAGTACCGGAGACAGGTGCAGTCACTCAC
  CTGTGAAGTGGATGCCCTTAAAGGCACTAACGAGTCCCTGGAGCGCCAGATGCGTGAGATGGAAGAGAAT
  TTTGCCCTTGAAGCTGCTAACTACCAGGACACTATTGGCCGCCTGCAGGATGAGATCCAAAACATGAAGG
  AAGAGATGGCTCGTCACCTTCGTGAATACCAAGATCTGCTCAATGTTAAGATGGCCCTGGACATTGAGAT
  CGCCACCTACAGGAAGCTGCTGGAAGGCGAGGAGAGCAGGATTTCTCTGCCTCTGCCAACCTTTTCTTCC
  CTGAACCTGAGAGAAACTAACCTGGAGTCACTTCCTCTGGTTGACACCCACTCAAAAAGAACACTCCTGA
  TTAAGACGGTTGAGACCAGAGATGGACAGGTGATCAATGAGACTTCTCAGCATCACGATGACCTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207446 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTRSVSSSSYRRMFGGSGTSSRPSSNRSYVTTSTRTYSLGSALRPSTSRSLYSSSPGGAYVTRSSAVRL
  RSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG
  QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDV
  DNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVA
  AKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEEN
  FALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPTFSS
  LNLRETNLESLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011701
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011701.1, NM_011701.2, NM_011701.3, NM_011701.4, NP_035831.2
• RefSeq Size: 1834
• RefSeq ORF: 1401
• Locus ID: 22352
• MW: 53.7 kDa
• Gene Summary: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. [UniProtKB/Swiss-Prot Function]