Steap4 (NM_054098) Mouse Tagged ORF Clone

CAT#: MR207521

Steap4 (Myc-DDK-tagged) - Mouse STEAP family member 4 (Steap4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Steap4
• Synonyms: 1110021O17Rik; AI481214; Tiarp; Tnfaip9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207521 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGCACATGCAGATGAGTTTCCTCTGACTACTGATTCCTCAGAAAAGCAAGGGGTTGTCTGCA
  TTTTCGGAACAGGGGATTTCGGGAAGTCACTGGGATTGAAAATGCTTCAGTGTGGCTATTCTATCGTTTT
  TGGGAGTCGAAACCCCCAGGTGTCCAGCCTTCTGCCCAGGGGGGCAGAGGTCCTGAGCTATTCGGAAGCA
  GCATCCAAGTCTGACATCATAATCCTAGCCATGCACAGAGAGCACTATGATTCCCTCACGGAACTAGTTG
  ATTATCTCAAAGGAAAAGTATTGGTAGACGTCAGTAACAACCGCAAAATCAATCAGTATCCAGAGTCAAA
  TGCGGAATACCTTGCTCAGCTGGAGCCAGGAGCCCACGTGGTCAAAGCATTTAACACCATCTCAGCCTGG
  GCTCTCCAGTCAGGAACACTAGATGCAAGCCGGCAGGTGTTTGTCTGTGGAAATGACAGCAAGGCCAAAC
  AAAGAGTAATGGACATTGCTCGTACTCTTGGGCTCACCCCACTGGACCAAGGATCTCTCATGGCAGCCAG
  TGAAATTGAAAACTACCCTCTGCAACTATTTCCAATGTGGAGGTTTCCTTTCTATTTGTCCTCTGTGCTG
  TGCGTCTTCTTCTTTGTGTACTGTGCAATACGAGAGGTGATATACCCCTACGTGAATGGGAAAACAGATG
  CTACGTACCGCCTGGCCATTTCCATCCCGAATCGTGTCTTTCCTATAACAGCACTGATCCTGCTTGCCTT
  GGTGTACCTCCCTGGTATTCTCGCTGCCATTCTGCAGCTCTATCGAGGCACAAAGTACCGCCGATTCCCA
  AACTGGCTTGACCATTGGATGCTTTGCAGAAAGCAACTGGGCTTGGTAGCTCTGGGATTTGCCTTCCTTC
  ATGTCATCTACACACTTGTGATTCCTATCCGTTACTATGTTCGATGGAGGCTGAGAAATGCAACCATTAC
  TCAGGCCCTAACTAATAAAGACAGCCCATTTATTACCTCTTATGCCTGGATTAATGATTCCTACTTGGCG
  CTGGGAATCCTGGGATTTTTTCTGTTCCTTCTGTTGGGAATCACTTCCTTGCCATCAGTAAGCAACATGG
  TCAACTGGAGAGAGTTCCGGTTTGTTCAGTCCAAACTGGGTTATCTGACCCTGGTCTTGTGCACAGCCCA
  CACTTTGGTGTATGGCGGAAAGAGATTCCTCAGCCCTTCGATTCTAAGGTGGAGCCTCCCTTCGGCTTAT
  ATTTTGGCACTCGTCATCCCCTGCGCAGTGCTGGTGCTGAAGTGCATCCTCATCATGCCATGCATAGACA
  AGACCCTCACTCGCATCCGCCAGGGCTGGGAAAGAAACTCAAAGTACACACAATCAGCACTGAACGGAAA
  ATCGGATATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207521 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKAHADEFPLTTDSSEKQGVVCIFGTGDFGKSLGLKMLQCGYSIVFGSRNPQVSSLLPRGAEVLSYSEA
  ASKSDIIILAMHREHYDSLTELVDYLKGKVLVDVSNNRKINQYPESNAEYLAQLEPGAHVVKAFNTISAW
  ALQSGTLDASRQVFVCGNDSKAKQRVMDIARTLGLTPLDQGSLMAASEIENYPLQLFPMWRFPFYLSSVL
  CVFFFVYCAIREVIYPYVNGKTDATYRLAISIPNRVFPITALILLALVYLPGILAAILQLYRGTKYRRFP
  NWLDHWMLCRKQLGLVALGFAFLHVIYTLVIPIRYYVRWRLRNATITQALTNKDSPFITSYAWINDSYLA
  LGILGFFLFLLLGITSLPSVSNMVNWREFRFVQSKLGYLTLVLCTAHTLVYGGKRFLSPSILRWSLPSAY
  ILALVIPCAVLVLKCILIMPCIDKTLTRIRQGWERNSKYTQSALNGKSDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054098
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054098.1, NM_054098.2, NM_054098.3, NP_473439.2
• RefSeq Size: 3140 bp
• RefSeq ORF: 1413 bp
• Locus ID: 117167
• MW: 53 kDa