Hhat (BC008159) Mouse Tagged ORF Clone

CAT#: MR207568

Hhat (Myc-DDK-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hhat
• Synonyms: MGC11697, Skn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207568 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCGGGTGGGAACTGACTCTCTGCCTGCTTGTTTCCTTGGGTTTCCACTTCCGCTCTTTCTATG
  AAGTTTACAAAGTCTCCAGAGAGCATGAAGAGGAACTGGATCAAGAATTTGAGCTGGAGATGGACACTCT
  ATTTGGAGGACTGAAGAAGGACCCGACTGATTTTGAGTGGAACTTCTGGATGGAATGGGGGAAGCGGAGG
  CTGGTGTGGCTCTTCATTGGCCATATGGCTGTGTCTCAGTTGGCCACGTTACTCACAAAGAAGCACAGAC
  CCTGGATTGTCATGGTCTATGGAATGTGGGCCTGCTGGTGTGTGCTGGGGGCGCCAGGCGTGGTCATGGT
  CCTTCTCCACAGCACCATCGCGTTCTGCGTGGCTCAGTTCCGATCCGTGCTCCTGTCCTGGCTGTGTTCT
  CTCCTTCTGCTCTCCACCCTAAGGCTGCAAAGTGTGGAGGAAGTTAAGAGAAGGTGGTACAAGACAGAGA
  ACGAGTATTACCTGCTCCAGTTTACGCTCACTGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT
  GTGCCGGCAGCCTCCGTCTGCACAACCTACTCCATCTGCGCAAGGTGCCTCCCATTCCTACCCGTGGCTG
  CTGACCTACGTCTTCTATTATCCCGTCTTCCACAACGGGCCCATCCTTAACTTCCCGGAGTTCTTCAGAC
  AGATGCAGCAGCCAGAGCTCAACTCTCTGCAGCACAGCCTCTGCATTGTAGCCAAGGGCCTGGGTCGTCT
  GCTCTGCTGGTGGTGGCTGGCTGAACTGATGGTACACCTCATGTACATGCATGCCCTCTACAGCAGCGCC
  CCCCTCCTGGAGAGCGTTTCCTGCTGGACCTTAGGAGGACTGGCGCTGGCCCAAGTGCTCTTCTTCTATG
  TGAAGTACCTGGTGCTCTTTGGTGTCCCGGCTCTGCTCATGCGCCTGGATGGACTCACGCCACCACCTCT
  TCCCCGCTGCGTGAGCACCATGTTCAGTTTTACCGGGATGTGGAGGTATTTTGATGTTGGACTACACAAT
  TTCTTAATCAGGTATGTGTACATCCCACTGGGCGGGTCCCAGCATGGCCTGTTGGGGACACTGCTTTCTA
  CTGCAACAACGTTTGCGTTCGTGAGCTACTGGCATGGTAGCTATGAGGACCTCTGGTGCTGGGCAGCGCT
  CAACTGGCTGGGAGTCACTGTGGAGAGTGGGGTCCGGAGGCTGCTAGAGACACCCTGTGTCCGGGAGACT
  TTGGCCCGGCACCTCTCCCCACAAGCCCACCATCGACTGCACGCTCTGCTTGCCGCCTGCTCTACCTCCA
  TGCTCATCCTCTTCAACCTGGTGTTCCTCGGGGGAATTCAAGTTGGGAAAACCTACTGGAACAGGATCTT
  CCTGCAAGCAGCCTTCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207568 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPGWELTLCLLVSLGFHFRSFYEVYKVSREHEEELDQEFELEMDTLFGGLKKDPTDFEWNFWMEWGKRR
  LVWLFIGHMAVSQLATLLTKKHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCS
  LLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWL
  LTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSA
  PLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHN
  FLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRET
  LARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQAAFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC008159
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008159, AAH08159
• RefSeq Size: 1832 bp
• RefSeq ORF: 1421 bp
• Locus ID: 226861
• MW: 54.8 kDa