Dmrt3 (NM_177360) Mouse Tagged ORF Clone

CAT#: MR207627

Dmrt3 (Myc-DDK-tagged) - Mouse doublesex and mab-3 related transcription factor 3 (Dmrt3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmrt3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207627 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCTACGGCTCCCCCTACCTGTACATGGGCGGCCCGGTGTCGCAGCCGCCGCGGGCGCCCTTGC
  AGCGCACGCCCAAGTGCGCGCGCTGCCGGAACCACGGGGTGCTGTCCTGGCTCAAGGGCCACAAGCGCTA
  CTGTCGCTTCAAGGACTGCACCTGCGAGAAGTGCATCCTGATCATCGAGCGGCAGCGGGTGATGGCGGCG
  CAGGTGGCGCTGCGCAGGCAGCAGGCCAACGAGAGCCTGGAGAGCCTCATCCCGGACTCGCTGCGGGCTC
  TGCCAGGGCCCCCGCCGCCGGGGGACGCTGCGGCCACCGCCGCTACCGCCTCGCAGTCGTCGCCAGCCTC
  CCAAGCGTCCCAGCCGCCCGCGCCGCCTCGTCCCACCGCGGAGTTGGCCGCGGCCGCCGCGCTGCGCTGG
  GTCGCGGAGCCCCAGCCCGGGACGCTGCCAGCGCAGCTTGCTAAACCAGATCTGACAGAAGAGAGAGTTG
  GGGACAGCAGTTCCACGGACAACACTGCGGAGGCCTTCAGTGACAAAGACACCGATCAGAGGAGCTCCCC
  AGATGTTGTCAAAAGCAAGAACTGCTTCACTCCGGAGAGCCCCGAGATCGTGTCCGTGGATGAAGGTGGG
  TACGCCGTGCAGAAAAACGGGGGCAACCCCGAGAGCTGCCCCGACAGCCCCAAGTACCACGCTGAGCAGA
  GCCACCTCCTGATCGAGGGCCCATCGGGGACCGTCTCTCTGCCCTTCAGCTTGAAAGCCAACCGGCCACC
  CCTGGAAGTGTTGAAGAAAATCTTTCCCAACCAGAAGCCCACTGTCCTGGAGCTCATCTTGAAAGGTTGC
  GGGGGCGACCTGGTCAGTGCCGTGGAGGTCCTGCTGTCCAGTCGCTCCTCCGCGGCTGGGGCGGAGCGGA
  CGGCCGAAGAGAGCCTGGTGCTGCCCTCTAGCGGCCATATCTTTGAACACACGCTGGGCTCCTACCCTAT
  CTCTTCCTCCAAGTGGTCCGTGGGCTCTGCCTTCCGTGTCCCTGACACCTTACGCTTTTCCGCAGACTCC
  AGCAACGTGGTCCCCAACCCCTTGGCCGTGCCCCTGCAGCATCCTTTCCCCCAGCCACCTCGGTACCCGC
  TGATGCTGAGGAATACTTTGGCCAGAAACCAGTCGAGCCCCTTTTTACCCAATGACGTCACCCTGTGGAA
  CACCATGACGCTGCAGCAGCAGTACCAGCTGAGGTCCCAGTATGTCAGTCCATTTCCCAGTAACTCCACT
  AGCGTCTTCCGGAGCTCGCCCGTGCTCTCCTCCCGCACCACCGAGGACCCTCGGATCTCCATCCCTGACG
  ACGGTTGTCCAATCGTGACAAAGCAGTCCATCTACACAGAGGATGACTATGACGAGAGGTCTGACTCCTC
  GGATTCTAGAATACTCAACACGTCATCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207627 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGYGSPYLYMGGPVSQPPRAPLQRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCEKCILIIERQRVMAA
  QVALRRQQANESLESLIPDSLRALPGPPPPGDAAATAATASQSSPASQASQPPAPPRPTAELAAAAALRW
  VAEPQPGTLPAQLAKPDLTEERVGDSSSTDNTAEAFSDKDTDQRSSPDVVKSKNCFTPESPEIVSVDEGG
  YAVQKNGGNPESCPDSPKYHAEQSHLLIEGPSGTVSLPFSLKANRPPLEVLKKIFPNQKPTVLELILKGC
  GGDLVSAVEVLLSSRSSAAGAERTAEESLVLPSSGHIFEHTLGSYPISSSKWSVGSAFRVPDTLRFSADS
  SNVVPNPLAVPLQHPFPQPPRYPLMLRNTLARNQSSPFLPNDVTLWNTMTLQQQYQLRSQYVSPFPSNST
  SVFRSSPVLSSRTTEDPRISIPDDGCPIVTKQSIYTEDDYDERSDSSDSRILNTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177360
• ORF Size: 1431 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177360.1, NM_177360.2, NM_177360.3, NP_796334.1, NP_796334.2
• RefSeq Size: 2401 bp
• RefSeq ORF: 1431 bp
• Locus ID: 240590
• MW: 51.5 kDa