Igh (BC028249) Mouse Tagged ORF Clone

CAT#: MR207728

Igh (Myc-DDK-tagged) - Mouse immunoglobulin heavy chain complex (cDNA clone MGC:40652 IMAGE:3487241)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Igh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207728 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATGGAGCTGTATCATGCTCTTCTTGGCAGCAACAGCTACAGGTGTCCACTCCCAGGTCCAACTGC
  AGCAGCCTGGGGCTGAGCTTGTGAAGCCTGGGGCTTCAGTAAAGCTGTCCTGCAAGGCTTCTGGCTACAC
  CTTCACCAGTTACTGGATGCACTGGGTGAAGCAGAGGCCTGGACGAGGCCTTGAGTGGATTGGAAGGATT
  GATCCTAATAGTGGTGGTACTAAGTACAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAAC
  CCTCCAGCACAGCCTACATGCAGCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTACAAG
  AGAGGGGGATTACGACGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAGAGCCTGCA
  AGAGAGCCCACCATCTACCCACTGACATTCCCACAAGCTCTGTCAAGTGACCCAGTGATAATCGGCTGCC
  TGATTCATGATTACTTCCCTTCCGGCACGATGAATGTGACCTGGGGAAAGAGTGGGAAGGATATAACCAC
  CGTAAACTTCCCACCTGCCCTGGCCTCTGGGGGACGGTACACCATGAGCAGCCAGTTGACCCTGCCAGCT
  GTCGAGTGCCCAGAAGGAGAATCCGTGAAATGTTCCGTGCAACATGACTCTAACCCCGTCCAAGAATTGA
  ACGTGAATTGCCCTGGTATCTGTTCTCCTCCTACTACTCCTCCTCCACCTTCCTGCCAGCCCAGCCTGTC
  ACTGCAGCGGCCAGCTCTTGAGGACCTGCTCCTGGGTTCAGATGCCAGCATCACATGTACTCTGAATGGC
  CTGAGAGATCCTGAGGGAGCTGTCTTCACCTGGGAGCCCTCCACTGGGAAGGATGCAGTGCAGAAGAAAG
  CTGTGCAGAATTCCTGCGGCTGCTACAGTGTGTCCAGCGTCCTGCCTGGCTGTGCTGAGCGCTGGAACAG
  TGGCGCATCATTCAAGTGCACAGTTACCCATCCTGAGTCTGACACCTTAACTGGCACAATTGCCAAAGTC
  ACAGTGAACACCTTCCCACCCCAGGTCCACCTGCTACCGCCGCCGTCGGAGGAGCTGGCCCTGAATGAGC
  TCGTGTCCCTGACATGCCTGGTGCGAGCTTTCAACCCTAAAGAAGTGCTGGTGCGATGGCTGCATGGAAA
  TGAGGAGCTGTCCCCAGAAAGCTACCTAGTGTTTGAGCCCCTAAAGGAGCCAGGCGAGGGAGCCACCACC
  TACCTGGTGACAAGCGTGTTGCGTGTATCAGCTGAAATCTGGAAACAGGGTGACCAGTACTCCTGCATGG
  TGGGCCACGAGGCCTTGCCCATGAACTTCACCCAGAAGACCATCGACCGTCTGTCGGGTAAACCCACCAA
  TGTCAGCGTGTCTGTGATCATGTCAGAGGGAGATGGCATCTGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207728 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGWSCIMLFLAATATGVHSQVQLQQPGAELVKPGASVKLSCKASGYTFTSYWMHWVKQRPGRGLEWIGRI
  DPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDSAVYYCTREGDYDAMDYWGQGTSVTVSSEPA
  REPTIYPLTFPQALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYTMSSQLTLPA
  VECPEGESVKCSVQHDSNPVQELNVNCPGICSPPTTPPPPSCQPSLSLQRPALEDLLLGSDASITCTLNG
  LRDPEGAVFTWEPSTGKDAVQKKAVQNSCGCYSVSSVLPGCAERWNSGASFKCTVTHPESDTLTGTIAKV
  TVNTFPPQVHLLPPPSEELALNELVSLTCLVRAFNPKEVLVRWLHGNEELSPESYLVFEPLKEPGEGATT
  YLVTSVLRVSAEIWKQGDQYSCMVGHEALPMNFTQKTIDRLSGKPTNVSVSVIMSEGDGICY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC028249
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC028249
• RefSeq Size: 1547
• RefSeq ORF: 1448
• Locus ID: 111507
• MW: 52.1 kDa
• Gene Summary: Summary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. The RefSeq represents the IGH locus from C57BL/6. Several V and D segments in C57BL/6 are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Jul 2008]