Il31ra (BC066164) Mouse Tagged ORF Clone

CAT#: MR207879

Il31ra (Myc-DDK-tagged) - Mouse interleukin 31 receptor A (cDNA clone MGC:76531 IMAGE:30475340)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il31ra
• Synonyms: NR10, GLM-R, Glmr, CRL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207879 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTCTGGGTGTGAACGCTGGAATAATGTGGACCTTGGCACTGTGGGCATTCTCTTTCCTCTGCA
  AATTCAGCCTGGCAGTCCTGCCGACTAAGCCAGAGAACATTTCCTGCGTCTTTTACTTCGACAGAAATCT
  GACTTGCACTTGGAGACCAGAGAAGGAAACCAATGATACCAGCTATATTGTGACTTTGACTTACTCCTAT
  GGAAAAAGCAATTATAGTGACAATGCTACAGAGGCTTCATATTCTTTTCCCCGTTCCTGTGCAATGCCCC
  CAGACATCTGCAGTGTTGAAGTACAAGCTCAAAATGGAGATGGTAAAGTTAAATCTGACATCACATATTG
  GCATTTAATCTCCATAGCAAAAACCGAACCACCTATAATTTTAAGTGTGAATCCAATTTGTAATAGAATG
  TTCCAGATACAATGGAAACCGCGTGAAAAGACTCGTGGGTTTCCTTTAGTATGCATGCTTCGGTTCAGAA
  CTGTCAACAGTAGCCACTGGACGGAAGTCAATTTTGAAAACTGTAAACAGGTCTGCAACCTCACAGGACT
  TCAGGCTTTCACAGAATATGTCCTGGCTCTACGGTTCAGGTTCAATGACTCAAGATATTGGAGCAAGTGG
  AGCAAAGAAGAAACCAGAGTGACTATGGAGGAAGTTCCACATGTCCTGGACCTGTGGAGAATTCTGGAAC
  CAGCAGACATGAACGGAGACAGGAAGGTGCGATTGTTGTGGAAGAAGGCAAGAGGAGCCCCCGTCTTGGA
  GAAAACATTTGGCTACCACATACAGTACTTTGCAGAGAACAGCACTAACCTCACAGAGATAAACAACATC
  ACCACCCAGCAGTATGAACTGCTTCTGATGAGCCAGGCACACTCTGTGTCCGTGACTTCTTTTAATTCTC
  TTGGCAAGTCCCAAGAGGCCATCCTGAGGATCCCAGATGTCCATGAGAAGACCTTCCAGTACATTAAGAG
  CATGAAGGCCTACATAGCCGAGCCCCTGTTGGTGGTGAACTGGCAAAGCTCCATTCCTGCGGTGGACACT
  TGGATAGTGGAGTGGCTCCCAGAAGCTGCCATGTCGAAGTTCCCTGCCCTTTCCTGGGAATCTGTGTCTC
  AGGTCACGAACTGGACCATCGAGCAAGATAAACTAAAACCTTTCACATGCTATAATATATCAGTGTATCC
  AGTGTTGGGACACCGAGTTGGAGAGCCGTATTCAATCCAAGCTTATGCCAAAGAAGGAACTCCATTAAAA
  GGTCCTGAGACCAGGGTGGAGAACATCGGTCTGAGGACAGCCACGATCACATGGAAGGAGATTCCTAAGA
  GTGCTAGGAATGGATTTATCAACAATTACACTGTATTTTACCAAGCTGAAGGTGGAAAAGAACTCTCCAA
  GACTGTTAACTCTCACTCACCCCAAGTTTATTACGCATTGACTGCTGCTAAATCAAGAAACTCCAGAAAC
  CTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207879 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNPLGVNAGIMWTLALWAFSFLCKFSLAVLPTKPENISCVFYFDRNLTCTWRPEKETNDTSYIVTLTYSY
  GKSNYSDNATEASYSFPRSCAMPPDICSVEVQAQNGDGKVKSDITYWHLISIAKTEPPIILSVNPICNRM
  FQIQWKPREKTRGFPLVCMLRFRTVNSSHWTEVNFENCKQVCNLTGLQAFTEYVLALRFRFNDSRYWSKW
  SKEETRVTMEEVPHVLDLWRILEPADMNGDRKVRLLWKKARGAPVLEKTFGYHIQYFAENSTNLTEINNI
  TTQQYELLLMSQAHSVSVTSFNSLGKSQEAILRIPDVHEKTFQYIKSMKAYIAEPLLVVNWQSSIPAVDT
  WIVEWLPEAAMSKFPALSWESVSQVTNWTIEQDKLKPFTCYNISVYPVLGHRVGEPYSIQAYAKEGTPLK
  GPETRVENIGLRTATITWKEIPKSARNGFINNYTVFYQAEGGKELSKTVNSHSPQVYYALTAAKSRNSRN
  LQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066164
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066164, AAH66164
• RefSeq Size: 2020 bp
• RefSeq ORF: 1478 bp
• Locus ID: 218624
• MW: 56.5 kDa