Cyp8b1 (NM_010012) Mouse Tagged ORF Clone

CAT#: MR208021

Cyp8b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 8, subfamily b, polypeptide 1 (Cyp8b1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp8b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208021 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGTGGTGTACAGTGCTAGGAGCCCTGCTCACGGTTGTGGGATGCCTGTGTCTGTCCTTGCTGC
  TCAGGCACCGCAGGCCCTGGGAGCCACCTCTGGACAAGGGTTTTGTGCCCTGGCTGGGTCACTCCATGGC
  TTTCCGGAAGAATATGTTTGAATTCTTGAAGGGGATGCGGGCCAAGCACGGGGATGTCTTCACGGTGCAG
  CTGGGAGGCCAGTACTTCACCTTTGTCATGGACCCCCTGTCCTTTGGCCCCATCATTAAGAACACAGAGA
  AAGCGCTGGACTTCCAGTCCTATGCAAAAGAACTGGTGCTCAAGGTGTTTGGGTACCAGTCTGTAGATGG
  GGACCACAGGATGATCCACTTAGCTAGTACCAAGCATCTGATGGGGCAAGGCTTGGAGGAACTCAACCAG
  GCCATGCTGGACAGCCTATCCTTGGTGATGCTAGGGCCTAAAGGTTCGAGTCTGGGTGCCAGCTCCTGGT
  GTGAAGATGGCCTCTTTCACTTCTGCTACAGAATCCTGTTTAAGGCTGGCTTCCTGAGCTTATTCGGCTA
  CACCAAGGACAAGCAGCAAGACCTGGATGAGGCCGATGAGCTGTTCAGGAAGTTCCGTCGATTTGACTTT
  CTCTTCCCCAGGTTTGTCTACTCCCTACTGGGGCCCCGGGAGTGGGTGGAAGTGAGCCAGTTGCAGCGTC
  TCTTCCACCAGAGACTCTCTGTGGAGCAAAACCTGGAAAAAGACGGCATAAGCTGTTGGTTAGGCTACAT
  GCTTCAGTTTCTGAGGGAGCAAGGAATAGCTTCATCCATGCAGGACAAATTTAACTTTATGATGCTCTGG
  GCCTCCCAGGGGAACACGGGACCCACCTGTTTCTGGGTCCTCTTATTCCTGCTGAAGCACCAGGATGCCA
  TGAAGGCTGTGCGTGAGGAAGCCACCAGGGTCATGGGTAAGGCCAGGCTGGAAGCCAAGAAGTCGTTCAC
  TTTCACTCCCAGTGCCCTGAAACACACTCCCGTGTTGGACAGCGTGATGGAGGAGAGTCTGCGTCTCTGT
  GCCACCCCCACCCTCCTCAGGGTGGTACAGGAGGATTATGTCCTGAAGATGGCCAGCGGGCAAGAGTACC
  AGATCCGTCGCGGAGATAAGGTGGCTCTCTTCCCCTATCTCTCAGTACACATGGACCCCGACATCCACCC
  TGAGCCCACAGCCTTCAAGTATGATCGGTTCCTCAACCCGGATGGCACCCGGAAAGTGGACTTCTACAAG
  TCGGGCAAGAAGATCCACCACTACAGCATGCCTTGGGGCTCGGGTGTTTCCAAGTGCCCCGGCAGGTTCT
  TTGCCCTCAGCGAGATGAAGACCTTTGTCCTTCTTATGATTATGTACTTCGACTTCAAGCTGGTCGACCC
  CGACATACCTGTGCCCCCCATTGACCCAAGGCGCTGGGGCTTTGGCACCTCACAGCCTAGCCATGAGGTA
  CGCTTCCTCTATCGCCTGAAGCCCGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208021 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLWCTVLGALLTVVGCLCLSLLLRHRRPWEPPLDKGFVPWLGHSMAFRKNMFEFLKGMRAKHGDVFTVQ
  LGGQYFTFVMDPLSFGPIIKNTEKALDFQSYAKELVLKVFGYQSVDGDHRMIHLASTKHLMGQGLEELNQ
  AMLDSLSLVMLGPKGSSLGASSWCEDGLFHFCYRILFKAGFLSLFGYTKDKQQDLDEADELFRKFRRFDF
  LFPRFVYSLLGPREWVEVSQLQRLFHQRLSVEQNLEKDGISCWLGYMLQFLREQGIASSMQDKFNFMMLW
  ASQGNTGPTCFWVLLFLLKHQDAMKAVREEATRVMGKARLEAKKSFTFTPSALKHTPVLDSVMEESLRLC
  ATPTLLRVVQEDYVLKMASGQEYQIRRGDKVALFPYLSVHMDPDIHPEPTAFKYDRFLNPDGTRKVDFYK
  SGKKIHHYSMPWGSGVSKCPGRFFALSEMKTFVLLMIMYFDFKLVDPDIPVPPIDPRRWGFGTSQPSHEV
  RFLYRLKPVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010012
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010012.1, NM_010012.2, NM_010012.3, NP_034142.2
• RefSeq Size: 1950 bp
• RefSeq ORF: 1503 bp
• Locus ID: 13124
• MW: 57.7 kDa