Ccdc132 (BC002304) Mouse Tagged ORF Clone

CAT#: MR208080

  • TrueORF®

Ccdc132 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 132 (cDNA clone MGC:8233 IMAGE:3591600)


  "BC002304" in other vectors (3)


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Reconstitution Protocol

USD 300.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ccdc132
Synonyms BLM, mKIAA1861
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208080 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTTAGAGAATGAGACATGGGAGCTTTGCCCTGTTAAGTCAAATTTTAGCATCTTGCAGCTTCATG
AGTTTAAATTCCTGGAACAGTCTCGCTCCCCATCAGTTTCACCTAGTAAGCAGCCATCAGCAACTTCTTC
AAAGCCTATGACTTTGTTTGAGCAGTACTGCAGTGGTGGGAATCCATTTGAAATACAAGCTGACCACAAA
GATGAAGAAACTGAAGATGTCTTAGCCTCTAATGGGTATGAGTCTGATGAGCAAGAGAAGAGCGCCTATC
AGGACTATGACAGTGACAGCGACGTCCCTGAGGAGCTGAAGCGGGATTACGTGGACGAGCAGACCGGCGA
CGTGCCCGTGAAGAGTGTTTCCCGAGAGACCCTGAAGAGCAGGAAGAAGTCGGATTACAGTCTAAACAAA
GTGAATGCACCCATCTTAACAAACACAACACTGAATGTAATAAGGCTTGTTGGAAAGTACATGCAGATGA
TGAACATCCTTAAGCCAATCGCCTTTGATGTGATCCATTTCATGTCTCAGCTCTTTGACTATTACTTGTA
TGCCATATATACCTTTTTTGGTCGGAATGATTCATTGGAATCAACAGGACTTGGCCTTAGTAGTAGCAGA
CTAAAAACAACTCTAAACAGAATTCAAGAAAGCCTTATTGACCTGGAGGGCTCAGCTGATCCAACTGCCA
CTCTCACAGCAGCCGAGGAAAGAAAGGAGAAGGTACCCAGCCCACACCTCAATCAGCTAGTGATTTTGAC
ATCTGGGGATACATTGTATGGGTTGGCAGAGAGAGTGGTAGCCACAGAATCCTTAGTGTTCTTGGCTGAG
CAGTTTGAGTTCCTTCAGCCACATTTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTCCTGCAGCAGT
TTTACTCCCAGACAGTCTCAACTGCCAGTGAACTGCGCAAGCCCATTTACTGGATTGTGGCTGGTAAAGC
TATTGATTATGAACAGATGCTTCTCCTAATGATGAATGTGAAGTGGGATGTCAAAGAAATCATGTCACAG
CACAACATCTATGTAGATGCATTGCTGAAGGAATTTGAGCAGTTTAACAAGCGGTTAAATGAAGTTTCTA
AGAGAGTTCGGATACCTCTGCCTGTATCAAATATACTTTGGGAGCATTGTATACGATTAGCTAACAGAAC
TATTGTGGAAGGATACGCCAATGTCAAGAAATGCAGTAATGAGGGGCGTGCCTTGATGCAATTGGATTTT
CAACAGTTTTTAATGAAACTCGAAAAACTAACTGACATTAGACCTATTCCTGATAAAGAGTTTGTGGAAA
CGTATATCAAAGCCTACTACCTAACTGAGAATGACATGGAGCGTTGGATCAAAGAGCACAGGGAATACTC
AACGAAGCAGTTGACCAACCTGGTGAATGTTTGCCTAGGATCTCATATCAATAAGAAAGCAAGACAGAAG
CTCCTAGCAGCTATAGATGAGATAGACAGGCCTAAAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208080 protein sequence
Red=Cloning site Green=Tags(s)

MFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSSKPMTLFEQYCSGGNPFEIQADHK
DEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNK
VNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSR
LKTTLNRIQESLIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAE
QFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQ
HNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDF
QQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQK
LLAAIDEIDRPKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC002304
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC002304, AAH02304
RefSeq Size 2124 bp
RefSeq ORF 1511 bp
Locus ID 73288
MW 58 kDa

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