Ccdc132 (BC002304) Mouse Tagged ORF Clone

CAT#: MR208080

Ccdc132 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 132 (cDNA clone MGC:8233 IMAGE:3591600)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc132
• Synonyms: BLM, mKIAA1861
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208080 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTAGAGAATGAGACATGGGAGCTTTGCCCTGTTAAGTCAAATTTTAGCATCTTGCAGCTTCATG
  AGTTTAAATTCCTGGAACAGTCTCGCTCCCCATCAGTTTCACCTAGTAAGCAGCCATCAGCAACTTCTTC
  AAAGCCTATGACTTTGTTTGAGCAGTACTGCAGTGGTGGGAATCCATTTGAAATACAAGCTGACCACAAA
  GATGAAGAAACTGAAGATGTCTTAGCCTCTAATGGGTATGAGTCTGATGAGCAAGAGAAGAGCGCCTATC
  AGGACTATGACAGTGACAGCGACGTCCCTGAGGAGCTGAAGCGGGATTACGTGGACGAGCAGACCGGCGA
  CGTGCCCGTGAAGAGTGTTTCCCGAGAGACCCTGAAGAGCAGGAAGAAGTCGGATTACAGTCTAAACAAA
  GTGAATGCACCCATCTTAACAAACACAACACTGAATGTAATAAGGCTTGTTGGAAAGTACATGCAGATGA
  TGAACATCCTTAAGCCAATCGCCTTTGATGTGATCCATTTCATGTCTCAGCTCTTTGACTATTACTTGTA
  TGCCATATATACCTTTTTTGGTCGGAATGATTCATTGGAATCAACAGGACTTGGCCTTAGTAGTAGCAGA
  CTAAAAACAACTCTAAACAGAATTCAAGAAAGCCTTATTGACCTGGAGGGCTCAGCTGATCCAACTGCCA
  CTCTCACAGCAGCCGAGGAAAGAAAGGAGAAGGTACCCAGCCCACACCTCAATCAGCTAGTGATTTTGAC
  ATCTGGGGATACATTGTATGGGTTGGCAGAGAGAGTGGTAGCCACAGAATCCTTAGTGTTCTTGGCTGAG
  CAGTTTGAGTTCCTTCAGCCACATTTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTCCTGCAGCAGT
  TTTACTCCCAGACAGTCTCAACTGCCAGTGAACTGCGCAAGCCCATTTACTGGATTGTGGCTGGTAAAGC
  TATTGATTATGAACAGATGCTTCTCCTAATGATGAATGTGAAGTGGGATGTCAAAGAAATCATGTCACAG
  CACAACATCTATGTAGATGCATTGCTGAAGGAATTTGAGCAGTTTAACAAGCGGTTAAATGAAGTTTCTA
  AGAGAGTTCGGATACCTCTGCCTGTATCAAATATACTTTGGGAGCATTGTATACGATTAGCTAACAGAAC
  TATTGTGGAAGGATACGCCAATGTCAAGAAATGCAGTAATGAGGGGCGTGCCTTGATGCAATTGGATTTT
  CAACAGTTTTTAATGAAACTCGAAAAACTAACTGACATTAGACCTATTCCTGATAAAGAGTTTGTGGAAA
  CGTATATCAAAGCCTACTACCTAACTGAGAATGACATGGAGCGTTGGATCAAAGAGCACAGGGAATACTC
  AACGAAGCAGTTGACCAACCTGGTGAATGTTTGCCTAGGATCTCATATCAATAAGAAAGCAAGACAGAAG
  CTCCTAGCAGCTATAGATGAGATAGACAGGCCTAAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208080 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSSKPMTLFEQYCSGGNPFEIQADHK
  DEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNK
  VNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSR
  LKTTLNRIQESLIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAE
  QFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQ
  HNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDF
  QQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQK
  LLAAIDEIDRPKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002304
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002304, AAH02304
• RefSeq Size: 2124 bp
• RefSeq ORF: 1511 bp
• Locus ID: 73288
• MW: 58 kDa