Sfrs8 (BC058689) Mouse Tagged ORF Clone

CAT#: MR208086

Sfrs8 (Myc-DDK-tagged) - Mouse splicing factor, arginine/serine-rich 8 (cDNA clone MGC:65672 IMAGE:6807331)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sfrs8
• Synonyms: SWAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208086 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGTCTGATGCCTTGCCTCAGAAATGTGAACAGGACTCCTTTGATGACATCCCCACCTGTAGACC
  ACACTTTCACACACACATGTGCACACTCACGCTGTGCCTTCCCGGCAGATGCTATCTGCCTATCAGCCAT
  CCCTCTTGCAAGTGGTAGTGCCTGTGACACTGTCTGCACATTCTGCTGCTGGTGCCTAAAAATACGTCAC
  AATTGTCACCTTTTCCCAACAGCTTCTTTTGCTCCAATAAGCTTTGCCATCAAAGCCAAAGAAAATGACC
  TCCTTCCCTTGGAGAAAAACCGTGTCAAGCTGGACGATGACAGTGAAGAGGATGAAGAAAGCAGGGAGTG
  CCAGGAAAGCACAAGCAGCGTGGCCAACCCCAGCCCTGCTGCCGCCCCGCCCTCCGTGGCTGTAGAGGAG
  AAGAAACCACAGCTTACCCAGGAGGAGCTGGAAGCCAAGCAAGCAAAACAAAAGCTGGAGGACCGCCTTG
  CTGCTGCTGCCCGGGAAAAGCTAGCCCAGGCGTCCAAGGAATCAAAGGAGAAGCAGCTTCAGGCTGAACG
  TAAAAGGAAAGCAGCGTTGTTCTTACAAACCTTAAAAAATCCTCTTCCAGAAGCAGAAGTTGGAAAACTT
  GAGGAGAGTACTTTCGGGGTTGAGGACACTGGTGTCATGCCCTGTCCTCTGCTGGTTGGTGGTAGAACTC
  TGCCCATATTGGAAGGGAAGCCTCCAGAAAGGCCTTCAAACAGATGCAGAGATCCACCAAGAGAAGAAGA
  AAGAGAGAAAAAGAAGAAGAAGCACAAAAAACGGTCTCGGACAAGGTCCCGCTCTCCCAAGTACCACTCG
  TCTTCCAAGCCCAGGTCTAGATCCCACTCAAAAGCAAAGCACTCCCTGCCCAGTGCCTACCGGACAGTGC
  GGCGGTCAAGGCCCTCAGCCTCCCCAGGGATCTTGAGTGTGGAGCCTTGTCCCAGCAGCTCGGCAGTGGC
  GGCAGCACAGCAGGAGAGTTCTCAGGCAGCCTCGGCCTCGAGCAGCTTTGACTCCTGGAGCTCTTTTGAG
  GGGAAACCTGGTAAACCGTCAAGGTCACGGTCTCGGTCCCCAAGAAGGAGAGCTCACTCTCCTGAGAGAC
  GGCGGGAAGAAAGGAGTGTCCCTACAGCCTACCGGATGAGCGGCAGCCCTGGGGTCAGCAGGAAAAGGAC
  CCGTTCCAGAAGTCCCCACGAGAAGAAGAAGAAACGGCGCTCACGGTCTCGCACCAAAGCCAAGGCCCGG
  TCGCAGTCAACATCCCCGAGTAAACAGGCCGCACAGCGTCCCTCGCCCCACTCAGCCCACTCGGCCAGCA
  TCTCTCCTGTGGAGAGCCGAGGTTCCTCCCAGGAACGCTCCAGGGGGGTTTCTCAGGAAAAAGATGGCCA
  GATCTCCTCAGCAATCGTCTCCTCTGTGCAGAGCAAAATAACTCAGGATCTCATGGCCAAAGTAAGAGCA
  ATGCTCGCAGCTTCAAAAAACCTGCAAACCAGTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208086 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSLMPCLRNVNRTPLMTSPPVDHTFTHTCAHSRCAFPADAICLSAIPLASGSACDTVCTFCCWCLKIRH
  NCHLFPTASFAPISFAIKAKENDLLPLEKNRVKLDDDSEEDEESRECQESTSSVANPSPAAAPPSVAVEE
  KKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQTLKNPLPEAEVGKL
  EESTFGVEDTGVMPCPLLVGGRTLPILEGKPPERPSNRCRDPPREEEREKKKKKHKKRSRTRSRSPKYHS
  SSKPRSRSHSKAKHSLPSAYRTVRRSRPSASPGILSVEPCPSSSAVAAAQQESSQAASASSSFDSWSSFE
  GKPGKPSRSRSRSPRRRAHSPERRREERSVPTAYRMSGSPGVSRKRTRSRSPHEKKKKRRSRSRTKAKAR
  SQSTSPSKQAAQRPSPHSAHSASISPVESRGSSQERSRGVSQEKDGQISSAIVSSVQSKITQDLMAKVRA
  MLAASKNLQTSAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058689
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058689, AAH58689
• RefSeq Size: 2180 bp
• RefSeq ORF: 1511 bp
• Locus ID: 231769
• MW: 55.3 kDa