Tcf4 (BC014293) Mouse Tagged ORF Clone

CAT#: MR208151

Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tcf4
• Synonyms: ME2, ITF-2, SEF-2, MITF-2A, MITF-2B, TFE, E2-2, E2.2, ITF2, SEF2, ASP-I2, ITF-2b, SEF2-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208151 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTGCGCATACACCATCCCGGGCATGGGCGGCAACTCTTTGATGTACTACTATAATGGGAAAGCGG
  TCTACGCTCCTTCAGCCAGCACTGCCGACTACAACAGGGATTCGCCAGGCTATCCTTCCTCCAAGCCAGC
  AGCCAGCACTTTCCCTAGCTCCTTCTTCATGCAAGATGGCCATCACAGCAGCGACCCTTGGAGCTCCTCC
  AGCGGGATGAATCAGCCCGGCTACGGAGGGATGCTGGGCAATTCTTCTCATATCCCACAGTCCAGCAGCT
  ACTGTAGCCTGCATCCACATGAACGTTTGAGCTATCCATCCCACTCCTCGGCAGACATCAACTCCAGTCT
  TCCTCCGATGTCCACGTTCCATCGTAGTGGCACAAACCATTACAGCACCTCTTCCTGCACACCCCCTGCC
  AACGGAACAGACAGTATAATGGCAAACAGAGGAACTGGGGCAGCAGGCAGCTCCCAGACTGGAGACGCTC
  TGGGGAAAGCCCTAGCTTCGATCTATTCTCCTGACCACACGAACAACAGCTTTTCCTCCAATCCTTCAAC
  TCCTGTGGGCTCCCCTCCTTCACTCTCAGCAGGCACAGCTGTTTGGTCTAGAAATGGAGGACAGGCCTCG
  TCATCTCCCAATTATGAAGGACCCTTGCACTCACTGCAAAGCCGAATCGAAGACCGTTTGGAAAGACTGG
  ACGATGCGATTCATGTTCTCCGGAACCACGCAGTGGGCCCGTCCACAGCTGTGCCTGGTGGGCATGGGGA
  CATGCATGGGATCATGGGACCCTCCCACAACGGAGCGATGGGTAGCCTGGGCTCAGGGTATGGAACTAGT
  CTTCTCTCAGCCAACAGACACTCGCTCATGGTTGGGGCCCACCGTGAAGATGGCGTGGCTCTGAGAGGCA
  GCCATTCTCTCCTGCCAAACCAGGTTCCGGTCCCACAACTTCCGGTCCAGTCTGCAACTTCCCCTGACTT
  GAACCCACCCCAAGACCCTTACAGAGGGATGCCACCAGGTCTCCAGGGCCAGAGCGTGTCTTCTGGTAGC
  TCTGAGATCAAATCCGACGACGAGGGCGATGAGAACCTGCAAGACACAAAATCTTCTGAGGACAAGAAAT
  TAGATGACGACAAGAAGGATATCAAATCAATTACTAGCAATAACGATGATGAGGACCTGACCCCAGAGCA
  GAAGGCTGAGCGCGAGAAGGAACGGAGGATGGCCAATAATGCCCGTGAGCGCCTGAGGGTCCGAGATATC
  AACGAGGCTTTCAAGGAGCTTGGCCGTATGGTGCAGCTCCACCTGAAGAGCGACAAGCCCCAGACCAAGC
  TCCTGATTCTCCACCAGGCCGTGGCTGTCATCCTCAGCCTGGAGCAGCAAGTTCGAGAAAGGAATCTGAA
  CCCGAAAGCTGCCTGTCTGAAAAGAAGGGAGGAAGAGAAGGTGTCCTCAGAGCCTCCCCCACTCTCCTTG
  GCTGGCCCACACCCTGGGATGGGAGACGCAGCGAATCACATGGGACAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208151 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYCAYTIPGMGGNSLMYYYNGKAVYAPSASTADYNRDSPGYPSSKPAASTFPSSFFMQDGHHSSDPWSSS
  SGMNQPGYGGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPA
  NGTDSIMANRGTGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQAS
  SSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAVPGGHGDMHGIMGPSHNGAMGSLGSGYGTS
  LLSANRHSLMVGAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGS
  SEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDI
  NEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSL
  AGPHPGMGDAANHMGQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC014293
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014293, AAH14293
• RefSeq Size: 1765 bp
• RefSeq ORF: 1523 bp
• Locus ID: 21413
• MW: 54.1 kDa