Tle6 (BC048832) Mouse Tagged ORF Clone

CAT#: MR208164

Tle6 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tle6
• Synonyms: Grg6, 1810057E06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208164 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATTGGTGTTGGGGCTGAAGGGGACAGTTGGCCATGTCATGTGTCTCATGAGGCCCCAATGGGCT
  CTGCCCAGACCACAGAGAACTCAGCTAAGGAAGAAGACAAACAGGTCCCAGAGTCTGCAGCCCTCCAGCA
  CCCCAAGTTCAAAAGCACCCCGGGGCCTCAGCTGCCCACCAGACGTCGGTTCCTCTCAGAGAGTGATGAG
  CTCCAGGACCCACAGCCGGTCTGGGACGCGGAGCCCCAGTTCTGTCAAGGCTTCCTGATCCAGGGACTCT
  GGGAACTTTTCATGGACAGCCGCCAAAAGAACCAACAGGAACACGGGGGTGAGGATTCCTCACAGGAAAG
  CAAAGATTCAGGCCTGTGTGACTTTAAACCAGAACCTCAGCCCAGGCACAGAAACAGCCTGAGTGACTCT
  GCCGACCCCTTCCTGATCAAGTCTCCCTCAGCTCTTCTAGATTATTACCAAGAAGACGTCAGTCGGCCCC
  AACCTGAGACGCAGGAATCTTCTGGAAGAGCTGATAAATTTCTGAAGCCCTTGTCCTGGGGCTCTGAAGT
  CTTAGAGAGCAGCTGTAATCAGCCTAGCACAGCACTCTGGCAATTAGAGAGGTTCACTGTCCCCCAAGCA
  CTGCAGAAGGTGCGTGTGCTGAAGCACCAGGAACTGCTATTGGTGGTAGCTGTGAGCTCCTTCACGCGGC
  ACGTCTTCACCTGCAGCCAGAGCGGCATCAAGGTGTGGAACCTCGTGAACCAGGTGGCCGAGGACAGAGA
  CCCAGAAAGCCACTTGAAATGCAGCGTCCAGGACAACAAGGTGTACCTGCGCACCTGCCTGCTGTCCTCC
  AACAGCAGGACCCTGTTCGCGGGTGGATACAACTTACCTGGCGTGATCGTGTGGGACCTGGCAGCCCCAT
  CCCTGTATGAGAAGTGCCAGCTGCCCTGCGAGGGCCTGTCCTGCCAGGCACTGGCCAACACAAAGGAAAA
  CATGGCCCTTGCCGGCTTCACAGATGGCACGGTCAGGATATGGGACCTACGGACTCAGGAAATAGTCAGG
  AACCTCAAAGGCCCTACCAATTCGGCCAGGAACCTTGTGGTCAAAGATGACAACATCTGGACAGGGGGTC
  TGGACGCCTGCCTGCGATGCTGGGACCTGCGGATGGCCAAGGTGTCACTGGAGCATCTGTTCCAGTCCCA
  GATCATGAGCCTGGCTCACAGTCCGACCGAAGACTGGCTGCTGCTGGGCCTGGCCAATGGCCAGCACTGC
  CTGTTCAACAGCAGGAAGAGGGACCAGGTGCTCACTGTGGACACCAAGGACAACACCATCTTAGGCCTCA
  AGTTCTCCCCAAATGGCAAGTGGTGGGCGAGCGTCGGTATGGGCAACTTCATCACGGTCCACAGCATGCC
  CACCGGAGCAAAACTGTTCCAGGTCCCCGAGGTTGGCCCTGTCAGATGCTTCGACATGACTGAGAACGGC
  AGGCTCATCATCACAGGATCCAGGGACTGTGCCTCGGTGTACCACATCAAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208164 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSIGVGAEGDSWPCHVSHEAPMGSAQTTENSAKEEDKQVPESAALQHPKFKSTPGPQLPTRRRFLSESDE
  LQDPQPVWDAEPQFCQGFLIQGLWELFMDSRQKNQQEHGGEDSSQESKDSGLCDFKPEPQPRHRNSLSDS
  ADPFLIKSPSALLDYYQEDVSRPQPETQESSGRADKFLKPLSWGSEVLESSCNQPSTALWQLERFTVPQA
  LQKVRVLKHQELLLVVAVSSFTRHVFTCSQSGIKVWNLVNQVAEDRDPESHLKCSVQDNKVYLRTCLLSS
  NSRTLFAGGYNLPGVIVWDLAAPSLYEKCQLPCEGLSCQALANTKENMALAGFTDGTVRIWDLRTQEIVR
  NLKGPTNSARNLVVKDDNIWTGGLDACLRCWDLRMAKVSLEHLFQSQIMSLAHSPTEDWLLLGLANGQHC
  LFNSRKRDQVLTVDTKDNTILGLKFSPNGKWWASVGMGNFITVHSMPTGAKLFQVPEVGPVRCFDMTENG
  RLIITGSRDCASVYHIKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048832
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048832, AAH48832
• RefSeq Size: 1897 bp
• RefSeq ORF: 1526 bp
• Locus ID: 114606
• MW: 56.8 kDa