Hspa14 (NM_015765) Mouse Tagged ORF Clone

CAT#: MR208190

Hspa14 (Myc-DDK-tagged) - Mouse heat shock protein 14 (Hspa14), transcript variant 1

Product Images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

• 

  Coomassie blue staining of purified Hspa14 protein (Cat# TP508190). The protein was produced from HEK293T cells transfected with Hspa14 cDNA clone (Cat# MR208190) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa14
• Synonyms: 70kDa; Hsp70-4; HSP70L1; hsr.1; NST-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208190 representing NM_015765
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATCGGCGTGCACCTGGGCTGCACATCAGCCTGTGTGGCTGTCTACAAGGATGGCCGGGCCG
  ATGTGGTTGCAAATGATGCAGGGGATAGAGTCACACCAGCCATTGTTGCTTACTCGGAACGTGAACAGGT
  GGTTGGACTGGCAGCAAAACAAAGTAGAATAAGACATGTTTCAAGCACAGTAGTGAAAGTGAAACAGATC
  CTTGGCAGAAGCTCTGCAGATCCACAAGCTCAGAAATACATCTCAGAAAGTAAATGCTTAGTCATTGAGA
  AAAATGGGAAGTTGCGGTATGAAATAGATACAGGAGAAGAAACAAAACTTGTTAATCCAGAAGATGTTGC
  CAGACTGATATTCAGTAAAATGAAAGAAACAGCGCATTCTGTCCTGGGCTCAGATGCAAATGACGTGGTT
  GTCACTGTCCCATTTGACTTTGGAGAAAAGCAGAAGTCTGCTCTGGGGGAGGCGGCTGGAGCTGCTGGAT
  TTAACGTGCTGCGGCTGATCCATGAGCCGTCTGCAGCTCTCCTTGCTTATGGGATTGGACAAGACCACCC
  TACCGGGAAGAGCAATGTTTTGGTGTTTAAGCTTGGAGGAACGTCCTTATCGCTCAGTGTCATGGAAGTT
  AACAGTGGGATGTATCGGGTCCTTTCCACAAACACCAGTGACAACATAGGGGGCGCACATTTCACAGACA
  CCTTAGCACAGTACCTGGCTTCGGAGTTCCAGAGGTTGTTCAAACATGATGTGAGAGGAAATGCCCGAGC
  CATGATGAAATTGATGAACAGCGCTGAAGTGGCCAAGCATTCTCTGTCAACCTTGGGAAGTGCCAACTGC
  TTTGTAGATTCCTTGTATGAAGGTCAAGACTTTGATTGCAATGTGTCCAGAGCAAGATTTGAGCTGCTCT
  GTTCTCCGCTTTTTAACAAATGTACAGAAGCAATCCGGGAGCTCTTGCGCCAGACTGGATTCACAGCAGA
  TGATATCAACAAGGTTGTGCTGTGTGGTGGATCTTCCAGAATCCCAAAGCTGCAGCAGCTGATTAAAGAT
  CTCTTCCCAGCCGTGGACCTCCTCAACTCTATCCCTCCAGATGAGGTCATCCCTATTGGTGCAGCCATAG
  AAGCCGGGATTCTTGTTGGGAAAGAGAGCACGTCAGGAGATGACTCTGTCATGATAGAGTGTTCAGCCAA
  GGATATTTTAGTTAAGGGAGTGGATGAGTCAGGTGCCGACAGATTCACAGTACTGTTTCCCTCTGGGACC
  CCGCTGCCAGCCCGAAGACAGCACACACTGCAGGCCCCCGGGAGGGTGTCTTCCGTTTGCCTTGAACTCT
  ATGAGTCTGAGGGGAAGAACTCTGCTAAAGAGGAGGCCAAGTTTGCACAGGTTGTACTCCAGGACTTAGA
  TAAAAAAGAAAATGGATTGCGTGATATATTAGCTGTTCTTACTATGAAAAGGGATGGATCTTTACAGGTG
  ACGTGCACAGACCAAGACACCGGCAAGTGTGAGGCCATTACTGTTGAGGTTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208190 representing NM_015765
  Red=Cloning site Green=Tags(s)
  
  MAAIGVHLGCTSACVAVYKDGRADVVANDAGDRVTPAIVAYSEREQVVGLAAKQSRIRHVSSTVVKVKQI
  LGRSSADPQAQKYISESKCLVIEKNGKLRYEIDTGEETKLVNPEDVARLIFSKMKETAHSVLGSDANDVV
  VTVPFDFGEKQKSALGEAAGAAGFNVLRLIHEPSAALLAYGIGQDHPTGKSNVLVFKLGGTSLSLSVMEV
  NSGMYRVLSTNTSDNIGGAHFTDTLAQYLASEFQRLFKHDVRGNARAMMKLMNSAEVAKHSLSTLGSANC
  FVDSLYEGQDFDCNVSRARFELLCSPLFNKCTEAIRELLRQTGFTADDINKVVLCGGSSRIPKLQQLIKD
  LFPAVDLLNSIPPDEVIPIGAAIEAGILVGKESTSGDDSVMIECSAKDILVKGVDESGADRFTVLFPSGT
  PLPARRQHTLQAPGRVSSVCLELYESEGKNSAKEEAKFAQVVLQDLDKKENGLRDILAVLTMKRDGSLQV
  TCTDQDTGKCEAITVEVAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015765
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015765.1, NM_015765.2, NP_056580.2
• RefSeq Size: 1791
• RefSeq ORF: 1530
• Locus ID: 50497
• MW: 54.7 kDa