Sirpa (NM_001177646) Mouse Tagged ORF Clone

CAT#: MR208194

Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sirpa
• Synonyms: AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208194 representing NM_001177646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCGGCCCGGCCCCTGGCCGCCTAGGGCCGCTGCTGCTCTGCCTGCTGCTCTCCGCGTCCT
  GTTTCTGTACAGGAGCCACGGGGAAGGAACTGAAGGTGACTCAGCCTGAGAAATCAGTGTCTGTTGCTGC
  TGGGGATTCGACCGTTCTGAACTGCACTTTGACCTCCTTGTTGCCGGTGGGACCCATTAGGTGGTACAGA
  GGAGTAGGGCCAAGCCGGCTGTTGATCTACAGTTTCGCAGGAGAATACGTTCCTCGAATTAGAAATGTTT
  CAGATACTACTAAGAGAAACAATATGGACTTTTCCATCCGTATCAGTAATGTCACCCCAGCAGATGCTGG
  CATCTACTACTGTGTGAAGTTCCAGAAAGGATCATCAGAGCCTGACACAGAAATACAATCTGGAGGGGGA
  ACAGAGGTCTATGTACTCGCCAAACCTTCTCCACCGGAGGTATCCGGCCCAGCAGACAGGGGCATACCTG
  ACCAGAAAGTGAACTTCACCTGCAAGTCTCATGGCTTCTCTCCCCGGAATATCACCCTGAAGTGGTTCAA
  AGATGGGCAAGAACTCCACCCCTTGGAGACCACCGTGAACCCTAGTGGAAAGAATGTCTCCTACAACATC
  TCCAGCACAGTCAGGGTGGTACTAAACTCCATGGATGTTAATTCTAAGGTCATCTGCGAGGTAGCCCACA
  TCACCTTGGATAGAAGCCCTCTTCGTGGGATTGCTAACCTGTCTAACTTCATCCGAGTTTCACCCACCGT
  GAAGGTCACCCAACAGTCCCCGACGTCAATGAACCAGGTGAACCTCACCTGCCGGGCTGAGAGGTTCTAC
  CCCGAGGATCTCCAGCTGATCTGGCTGGAGAATGGAAACGTATCACGGAATGACACGCCCAAGAATCTCA
  CAAAGAACACGGATGGGACCTATAATTACACAAGCTTGTTCCTGGTGAACTCATCTGCTCATAGAGAGGA
  CGTGGTGTTCACGTGCCAGGTGAAGCACGACCAACAGCCAGCGATCACCCGAAACCATACCGTGCTGGGA
  TTTGCCCACTCGAGTGATCAAGGGAGCATGCAAACCTTCCCTGATAATAATGCTACCCACAACTGGAATG
  TCTTCATCGGTGTGGGCGTGGCGTGTGCTTTGCTCGTAGTCCTGCTGATGGCTGCTCTCTACCTCCTCCG
  GATCAAACAGAAGAAAGCCAAGGGGTCAACATCTTCCACACGGTTGCACGAGCCCGAGAAGAACGCCAGG
  GAAATAACCCAGATCCAGGACACAAATGACATCAACGACATCACATACGCAGACCTGAATCTGCCCAAAG
  AGAAGAAGCCCGCACCCCGGGCCCCTGAGCCTAACAACCACACAGAATATGCAAGCATTGAGACAGGCAA
  AGTGCCTAGGCCAGAGGATACCCTCACCTATGCTGACCTGGACATGGTCCACCTCAGCCGGGCACAGCCA
  GCCCCCAAGCCTGAGCCATCTTTCTCAGAGTATGCTAGTGTCCAGGTCCAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208194 representing NM_001177646
  Red=Cloning site Green=Tags(s)
  
  MEPAGPAPGRLGPLLLCLLLSASCFCTGATGKELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYR
  GVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGG
  TEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNI
  SSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFY
  PEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLG
  FAHSSDQGSMQTFPDNNATHNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAR
  EITQIQDTNDINDITYADLNLPKEKKPAPRAPEPNNHTEYASIETGKVPRPEDTLTYADLDMVHLSRAQP
  APKPEPSFSEYASVQVQRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177646
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177646.1, NP_001171117.1
• RefSeq Size: 3835 bp
• RefSeq ORF: 1530 bp
• Locus ID: 19261
• MW: 56.4 kDa