Lap3 (NM_024434) Mouse Tagged ORF Clone

CAT#: MR208318

Lap3 (Myc-DDK-tagged) - Mouse leucine aminopeptidase 3 (Lap3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lap3
• Synonyms: 2410015L10Rik; AA410100; Lap; LAP-3; Lapep; Pep-7; Pep-S; Pep7; Peps
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208318 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTTGCTGCCCCTTCCGGCTGCCGCGCGAGTCGCGCTCCGCCGCCTGGGAGTGAGAGGTCTCTGGG
  ATCGGGGTCTGTCCACCGCAGACATGACGAAGGGCCTTGTTTTAGGAATTTATGCCAAAGACAAAGATGA
  TGACCTGCCACAATTTACAAGTGCGGGAGAGAGTTTTAATAAGTTGGTGTCTGGAAAGCTGAGGGAAATG
  TTGAACATATCTGGACCTCCTCTCAAAGCAGGCAAAACCAGAACCTTCTATGGTCTGCATCAGGACTTCC
  CCAGCGTGGTGGTGGTCGGCCTTGGCAAGCGATCGGCTGGAGTTGATGACCAGGAAAACTGGCACGAAGG
  CAAAGAAAACATCAGAGCTGCTGTTGCAGCGGGATGCAGGCAGGTCCAAGACCTGGAGCTGCCGTCGGTG
  GAGGTGGATCCCTGTGGAGATGCGCAAGCTGCTGCAGAAGGAGCTGTGCTTGGTCTCTATGAGTACGATG
  ACCTGAAGCAGAAGAAGAAGGTGGCTGTGTCGGCGAAGCTCCACGGAAGCGGTGATCTCGAGGCCTGGGA
  GAAAGGGGTCCTCTTTGCTTCTGGACAGAACTTGGCCCGTCATCTGATGGAGTCTCCAGCCAATGAGATG
  ACACCAACCAGATTCGCTGAAATCATTGAAAAAAACCTCAAAAGTGCCAGTAGTAAAACCAAGGTCCATA
  TCAGACCCAAGTCCTGGATAGAGGAACAAGAAATGGGTTCGTTCCTAAGTGTAGCCAAGGGGTCTGAAGA
  ACCACCAGTCTTCTTGGAAATCCACTACATGGGCAGCCCCAATGCAACCGAGGCACCCCTGGTGTTTGTG
  GGGAAGGGCATTACCTTTGACAGTGGAGGTATCTCCATCAAGGCTTCTGCAAACATGGACCTCATGAGGG
  CTGATATGGGAGGAGCAGCAACAATATGCTCGGCCATTGTGTCTGCGGCAAAGCTCAACTTGCCCATTAA
  CATCATTGGTTTGGCCCCTCTTTGTGAAAATATGCCTAGCGGCAAGGCCAACAAGCCAGGGGATGTCGTC
  AGAGCCAGGAATGGGAAGACCATCCAGGTTGATAACACTGACGCCGAGGGGAGGCTCATCCTGGCCGATG
  CACTCTGTTATGCACACACCTTTAATCCCAAGGTCATCATCAATGCTGCCACCTTAACAGGTGCCATGGA
  TGTAGCCCTGGGGTCAGGTGCCACTGGAGTCTTTACCAATTCGTCCTGGCTGTGGAACAAACTATTTGAG
  GCCAGCGTAGAAACTGGGGACCGAGTCTGGAGGATGCCCCTCTTTGAGCATTATACAAGACAAGTCATAG
  ATTGCCAGCTTGCTGATGTCAACAATCTTGGAAAATACAGATCTGCAGGTGCGTGTACAGCTGCCGCATT
  CCTGCGAGAGTTTGTGACACATACGAAGTGGGCACACTTAGACATAGCAGGAGTGATGACCAACAAAGAT
  GAGATTCCCTACCTGCGGAAGGGGATGAGTGGGCGGCCCACGAGGACTCTGATTGAGTTCTTACTTCGAT
  TCAGTAAAGACAGTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208318 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYLLPLPAAARVALRRLGVRGLWDRGLSTADMTKGLVLGIYAKDKDDDLPQFTSAGESFNKLVSGKLREM
  LNISGPPLKAGKTRTFYGLHQDFPSVVVVGLGKRSAGVDDQENWHEGKENIRAAVAAGCRQVQDLELPSV
  EVDPCGDAQAAAEGAVLGLYEYDDLKQKKKVAVSAKLHGSGDLEAWEKGVLFASGQNLARHLMESPANEM
  TPTRFAEIIEKNLKSASSKTKVHIRPKSWIEEQEMGSFLSVAKGSEEPPVFLEIHYMGSPNATEAPLVFV
  GKGITFDSGGISIKASANMDLMRADMGGAATICSAIVSAAKLNLPINIIGLAPLCENMPSGKANKPGDVV
  RARNGKTIQVDNTDAEGRLILADALCYAHTFNPKVIINAATLTGAMDVALGSGATGVFTNSSWLWNKLFE
  ASVETGDRVWRMPLFEHYTRQVIDCQLADVNNLGKYRSAGACTAAAFLREFVTHTKWAHLDIAGVMTNKD
  EIPYLRKGMSGRPTRTLIEFLLRFSKDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024434
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024434.1, NM_024434.2, NM_024434.3, NM_024434.4, NM_024434.5, NM_024434.6, NP_077754.2
• RefSeq Size: 2267 bp
• RefSeq ORF: 1560 bp
• Locus ID: 66988
• MW: 56.1 kDa