Uap1 (BC017547) Mouse Tagged ORF Clone

CAT#: MR208347

Uap1 (Myc-DDK-tagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uap1
• Synonyms: AGX-1, AGX-2, AgX, AGX1, SPAG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTCAATGACCTCAAACAAAGGCTGTCCCAAGCTGGGCAAGAGCACCTGCTGCAGTTCTGGAATG
  AGCTGTCCGAAGCCCAGCAGGTAGAACTTTATATGGAGCTCCAGGCCATGAACTTTGAAGAGCTGAACTC
  CTTTTTCCGGAAGGCCATTGGTGAATTTGATCGATCCTCTCACCAAGAAAAGGTGGACGCACGAATGGAG
  CCTGTGCCCCGGCAGGTTTTGGGCAGTGCTACCAGAGATCAAGAGCAGCTGCAGGCCTGGGAAAGTGAAG
  GACTTTCTCAGATTTCTCAAAACAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTTGG
  AGTCTCATATCCCAAGGGCATGTATGATGTTGGCTTGCCATCCCACAAGACGCTTTTTCAGATTCAAGCA
  GAACGTATTCTGAAACTCCAGCAGTTAGCTGAAAAACACCATGGCAACAAGTGCACCATCCCATGGTATA
  TAATGACCAGCGGCAGAACAATGGAATCAACGAAGGAGTTCTTCACAAAGCACAAGTTCTTTGGTTTAAA
  AAAGGAGAACGTGGTGTTTTTCCAGCAGGGAATGCTGCCTGCTATGAGTTTTGATGGGAAAATTATCTTG
  GAAGAGAAAAACAAAGTTTCTATGGCTCCAGATGGGAATGGTGGTCTCTACCGGGCTCTTGCAGCCCAAA
  ACATTGTGGAGGACATGGAGCAAAGAGGCATCTGCAGTATTCATGTCTATTGTGTGGACAACATATTGGT
  AAAAGTGGCGGACCCACGGTTCATTGGATTCTGCATTCAGAAAGGAGCAGACTGTGGGGCAAAGGTGGTA
  GAGAAAACGAATCCCACAGAGCCAGTTGGAGTGGTTTGCCGGGTGGATGGAGTATACCAGGTGGTAGAAT
  ACAGCGAGATCTCTCTGGCAACAGCTCAAAGGCGAAGTTCAGATGGACGGCTGCTGTTCAATGCAGGGAA
  CATTGCCAATCACTTCTTCACTGTCCCGTTCCTGAAAGATGTTGTCAATGTTTATGAACCTCAGTTGCAG
  CACCATGTGGCTCAAAAGAAGATTCCATATGTGGACTCCCAGGGGTACTTCATTAAGCCAGACAAGCCAA
  ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTGTATGAAGT
  ATTACGGGAAGATGAGTTTTCCCCGCTCAAGAATGCAGACAGTCAGAACGGGAAGGACAACCCGACCACG
  GCAAGGCATGCTTTGATGTCCCTTCACCACTGCTGGGTCCTCAATGCCGGGGGACACTTCATAGATGAGA
  ATGGCTCTCGCCTCCCAGCCATCCCCCGTGCTACAAATGGAAAGTCAGAGGCCATCACAGCTGATGTCAA
  TCACAACTTGAAAGATGCCAATGATGTACCGATCCAGTGTGAAATCTCTCCCCTCATCTCTTATGCTGGA
  GAGGGTCTTGAAGGTTATGTGGCAGATAAAGAGTTCCATGCACCGTTAATCATCGATGAGAATGGAGTTC
  ATGAGCTGGTGAAAAATGGCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNVNDLKQRLSQAGQEHLLQFWNELSEAQQVELYMELQAMNFEELNSFFRKAIGEFDRSSHQEKVDARME
  PVPRQVLGSATRDQEQLQAWESEGLSQISQNKVAVLLLAGGQGTRLGVSYPKGMYDVGLPSHKTLFQIQA
  ERILKLQQLAEKHHGNKCTIPWYIMTSGRTMESTKEFFTKHKFFGLKKENVVFFQQGMLPAMSFDGKIIL
  EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGICSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV
  EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQRRSSDGRLLFNAGNIANHFFTVPFLKDVVNVYEPQLQ
  HHVAQKKIPYVDSQGYFIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT
  ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRATNGKSEAITADVNHNLKDANDVPIQCEISPLISYAG
  EGLEGYVADKEFHAPLIIDENGVHELVKNGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC017547
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017547, AAH17547
• RefSeq Size: 2139 bp
• RefSeq ORF: 1565 bp
• Locus ID: 107652
• MW: 58.5 kDa