Ears2 (NM_026140) Mouse Tagged ORF Clone

CAT#: MR208373

Ears2 (Myc-DDK-tagged) - Mouse glutamyl-tRNA synthetase 2 (mitochondrial)(putative) (Ears2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ears2
• Synonyms: 3230401I01Rik; mKIAA1970
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208373 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCCTCAAGAGGCTTCTGCTGGCCGAGCCGCACGTGGTGGCCCTCGGGCACCGCGTGGGGC
  GGCGCGAGGCCAGTCTGGGCCCAGATCCCGGGGCCCCGGTGCGAGTGCGCTTCGCTCCCAGCCCCACAGG
  CTTCCTGCACCTGGGTGGCCTCCGTACTGCCCTCTACAACTACATCTTTGCCAAGAAGCACCAAGGGAGC
  TTCATTCTGAGGCTGGAGGACACCGATCAGAGCCGCTTGGTGCCAGGGGCAGCAGAAAGCATTGAGGACA
  TGCTGGAGTGGGCAGGCATCCCACCTGATGAGAGTCCCCGCCGGGGTGGTCCTGCAGGGCCCTACTGTCA
  GTCCCAGCGGCTGGCTCTGTATGCCCAAGCCACGGAAGCCTTGCTGAGATCTGGAGCTGCCTATCCCTGT
  TTCTGTTTGCCACAGCGGCTGGAGCTTCTGAAGAAGGAAGCACTGAGGAGCCGACAGACACCCCGGTATG
  ACAATCGGTGCCGGAACCTGAGCCAGGCACAGGTGGCCCAGAAGCTGGCCGTAGACCCCAAGCCTGCTAT
  CCGCTTCCGTCTAGAGGAGGCAGTACCAGCCTTCCAGGACCTGGTATATGGCTGGACTCAGCATGAAGTG
  GCCAGTGTGGAGGGGGACCCAGTTATCCTGAAAAGTGACGGCTTTCCCACCTACCACCTAGCCTGTGTGG
  TAGATGACCACCACATGAGCATCAGCCATGTGCTGCGGGGCTCCGAGTGGCTAGTCTCCACCTCCAAGCA
  CCTGCTCCTCTACCAGGCCCTGGGCTGGCAGCCACCTCGATTTGCTCATCTGCCCCTGCTCCTCAACAGG
  GATGGTAGCAAGCTGTCTAAGAGGCAAGGGGACATTTTCCTGGAACATTTTGCTGCCACCGGTTTTCTGC
  CAGAAGCCTTGCTTGACATCATCACCAACTGTGGCTCGGGGTTTGCAGAGAACCAAATGGGCAGGACCCT
  GCCGGAGCTGATTACACAGTTTGACCTGACGCGGATCACCTGCCACTCAGCCCTGCTGGACCTAGAGAAG
  CTCCCAGAATTTAACAGGCTGCACCTCCGCCGGCTGGTGAGCAGTGAGACCCAGAGGCCCCAGTTGGTGG
  AGAAATTGCAGGGCCTGGTGAAGGAGGCATTCGGGAGTGAACTGCAAAACAAGGATGTTCTGGACCCTGC
  ATATATGGAGAGGATCCTCCTGCTGAGACAGGGTCACATCAGCCGCCTGCAGGATCTGGTCTCCCCAGTG
  TACTCCTACTTATGGACCCGCCCTGCTGTCCATCGATCAGAACTGGGTGCCAGCTCGGAAAACGTGGATG
  TGATTGCCAAGCGCTTGCTGGGGCTTTTAGAAAGACCTGGCTTAAGCCTAACTCAGGATGTGCTGAACAG
  AGAGCTGAAGAAGCTGTCAGAAGGTCTGGAAGGCGCCAAGCATAGCAGTGTGATGAAGCTCCTCCGGATG
  GCCCTCAGTGGACAGCTGCAAGGACCCCCTGTGGCTGAGATGATGGTGTCCTTGGGCCCAAAGGAAGTAC
  GGGAACGAATCCAGAAGGTGCTTTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPLKRLLLAEPHVVALGHRVGRREASLGPDPGAPVRVRFAPSPTGFLHLGGLRTALYNYIFAKKHQGS
  FILRLEDTDQSRLVPGAAESIEDMLEWAGIPPDESPRRGGPAGPYCQSQRLALYAQATEALLRSGAAYPC
  FCLPQRLELLKKEALRSRQTPRYDNRCRNLSQAQVAQKLAVDPKPAIRFRLEEAVPAFQDLVYGWTQHEV
  ASVEGDPVILKSDGFPTYHLACVVDDHHMSISHVLRGSEWLVSTSKHLLLYQALGWQPPRFAHLPLLLNR
  DGSKLSKRQGDIFLEHFAATGFLPEALLDIITNCGSGFAENQMGRTLPELITQFDLTRITCHSALLDLEK
  LPEFNRLHLRRLVSSETQRPQLVEKLQGLVKEAFGSELQNKDVLDPAYMERILLLRQGHISRLQDLVSPV
  YSYLWTRPAVHRSELGASSENVDVIAKRLLGLLERPGLSLTQDVLNRELKKLSEGLEGAKHSSVMKLLRM
  ALSGQLQGPPVAEMMVSLGPKEVRERIQKVLSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026140
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_026140.1, NM_026140.2, NM_026140.3, NP_080416.1
• RefSeq Size: 1862
• RefSeq ORF: 1572
• Locus ID: 67417
• MW: 58.3 kDa
• Gene Summary: This gene encodes a putative member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in a similar gene in human have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). [provided by RefSeq, Mar 2015]