Gk (NM_008194) Mouse Tagged ORF Clone

CAT#: MR208395

Gyk (Myc-DDK-tagged) - Mouse glycerol kinase (Gyk), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gk
• Synonyms: D930012N15Rik; Gyk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208395 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGCGAAGAAAGCAGTTCTGGGGCCATTGGTGGGAGCAGTGGACCAGGGTACCAGCTCGACAC
  GTTTTTTGGTTTTCAATTCAAAAACAGCTGAACTTCTTAGTCATCATCAAGTAGAAATAAAACAGGAATT
  CCCAAGAGAAGGATGGGTAGAACAAGACCCGAAGGAAATTCTGCAGTCTGTTTATGAGTGTATAGAGAAA
  ACGTGTGAGAAACTTGGACAGCTCAATATTGATATTTCCAACATCAAAGCCATTGGTGTCAGCAACCAGA
  GGGAAACCACAGTAGTCTGGGACAAGGTCACCGGAGAGCCTCTCTATAATGCCGTGGTGTGGCTTGACCT
  AAGAACCCAGTCTACTGTTGAGAACCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAG
  ACAGGCCTTCCACTTAGCACGTATTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAACGTGAAAAAGG
  TCCAAGAGGCTGTTGAAGAAAATAGAGCTCTTTTTGGGACCATTGATTCATGGCTTATTTGGAGTTTAAC
  AGGAGGAATCCATGGGGGTGTCCACTGTACAGATGTAACAAATGCAAGCAGGACGATGCTTTTTAACATT
  CATTCTTTGGAATGGGATAAAGAGCTCTGCGAATTTTTTGGAATTCCAATGGAAATTCTTCCCAACGTTC
  GGAGTTCTTCTGAGATCTATGGCCTAATGAAAGCTGGGGCCTTGGAAGGTGTACCAATATCTGGGTGTTT
  GGGGGACCAGTCTGCTGCTTTGGTGGGACAAATGTGCTTCCAGGATGGACAGGCCAAAAACACGTATGGA
  ACAGGGTGCTTCTTATTGTGCAACACGGGCCATAAGTGTGTATTTTCTGAACATGGCCTCCTGACAACCG
  TAGCATATAAACTTGGCAGAGACAAACCTGTGTATTATGCGTTGGAAGGTTCCGTGGCTATAGCTGGTGC
  TGTAATCCGCTGGCTAAGAGACAACCTTGGAATTATTAAGTCCTCTGAGGAAATTGAAAAACTTGCTAAG
  GAAGTAGGTACTTCTTATGGCTGCTACTTCGTTCCAGCATTTTCAGGGTTATATGCGCCTTATTGGGAGC
  CCAGTGCAAGAGGGATCATCTGTGGACTCACTCAGTTCACCAATAAATGTCATATCGCTTTTGCTGCACT
  AGAAGCTGTTTGTTTCCAAACCCGAGAGATTTTGGATGCCATGAATCGCGACTGTGGAATTCCACTCAGT
  CATTTACAGGTAGATGGAGGAATGACCAGCAATAAAATTCTTATGCAGCTACAAGCAGACATTCTGTATA
  TTCCAGTAGTGAAACCCTCCATGCCTGAAACAACTGCACTAGGCGCTGCCATGGCAGCTGGGGCTGCAGA
  GGGGGTTGGTGTGTGGAGTCTTGAACCTGAGGATTTGTCAGCTGTCACAATGGAGCGGTTTGAACCTCAG
  ATCAATGCTGAAGAAAGCGAAATCCGTTACTCCACATGGAAGAAAGCTGTGATGAAGTCAATTGGTTGGG
  TTACAACTCAGTCTCCAGAAAGTGGTATCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208395 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILQSVYECIEK
  TCEKLGQLNIDISNIKAIGVSNQRETTVVWDKVTGEPLYNAVVWLDLRTQSTVENLSKRIPGNNNFVKSK
  TGLPLSTYFSAVKLRWLLDNVKKVQEAVEENRALFGTIDSWLIWSLTGGIHGGVHCTDVTNASRTMLFNI
  HSLEWDKELCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQDGQAKNTYG
  TGCFLLCNTGHKCVFSEHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKSSEEIEKLAK
  EVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRDCGIPLS
  HLQVDGGMTSNKILMQLQADILYIPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQ
  INAEESEIRYSTWKKAVMKSIGWVTTQSPESGIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008194
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008194.1, NM_008194.2, NM_008194.3, NP_032220.1
• RefSeq Size: 4357
• RefSeq ORF: 1575
• Locus ID: 14933
• MW: 57.5 kDa