Kpna2 (BC003274) Mouse Tagged ORF Clone

CAT#: MR208477

Kpna2 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:6031 IMAGE:3499880)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kpna2
• Synonyms: IPOA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208477 representing BC003274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACGAACGAGAATGCTAACTTACCAGCTGCCCGACTTAACAGGTTCAAGAACAAGGGGAAGGACA
  GCACAGAAATGCGTCGCCGCCGAATAGAAGTTAATGTGGAACTCAGGAAAGCTAAAAAAGATGAGCAGAT
  GCTGAAAAGAAGAAACGTCAGCTCCTTTCCTGATGATGCTACTTCTCCGCTACAGGAAAACCGGAACAAC
  CAGGGTACTGTAAATTGGTCTGTTGAGGACATTGTTAAAGGCATAAACAGTAACAATTTGGAAAGCCAGC
  TCCAAGCTACTCAAGCTGCTCGGAAATTGCTTTCTAGAGAGAAACAGCCTCCTATAGACAACATCATCCG
  GGCTGGTTTGATCCCAAAATTTGTGTCCTTCTTGGGCAAAACTGATTGTAGTCCTATTCAGTTTGAGTCT
  GCTTGGGCACTCACCAACATTGCTTCTGGAACATCTGAACAGACCAAAGCTGTGGTGGATGGAGGTGCTA
  TCCCAGCGTTTATTTCTCTCTTGGCATCTCCTCATGCTCACATCAGCGAGCAAGCTGTTTGGGCTCTTGG
  AAACATTGCAGGTGATGGTTCAGCTTTCCGAGACTTAGTTATCAAACACGGTGCGATTGACCCACTGTTG
  GCACTTCTTGCAGTTCCCGATCTGTCTACCTTGGCATGTGGTTACTTACGTAATCTTACCTGGACGCTTT
  CAAACCTTTGTCGAAACAAGAACCCTGCACCTCCCTTAGATGCCGTTGAGCAGATTCTTCCTACGTTAGT
  TCGACTCCTGCACCACAATGATCCAGAAGTATTGGCAGATTCCTGCTGGGCCATTTCCTACCTGACTGAT
  GGTCCAAATGAGCGCATTGAGATGGTTGTGAAGAAAGGAGTTGTTCCCCAACTTGTGAAGCTTCTAGGAG
  CTACTGAACTGCCCATTGTGACTCCCGCACTAAGAGCCATAGGGAATATTGTCACTGGAACAGATGAGCA
  GACTCAGAAAGTGATCGATGCAGGAGCACTTGCAGTCTTTCCCAGCCTGCTAACAAACCCCAAAACTAAT
  ATTCAGAAGGAGGCCACATGGACAATGTCGAACATTACAGCTGGACGCCAGGACCAGATACAGCAAGTTG
  TGAATCACGGCCTAGTCCCCTTTCTTGTTGGTGTCCTCTCTAAGGCGGACTTTAAGACACAGAAGGAGGC
  CGCGTGGGCTATAACCAACTATACCAGCGGTGGGACTGTTGAGCAGATTGTGTATCTCGTTCACTGTGGG
  ATAATAGAACCTTTGATGAACCTCCTGAGTGCAAAAGATACCAAGATTATTCAGGTTATTCTTGACGCCA
  TTTCAAATATCTTTCAGGCTGCAGAGAAACTAGGTGAGACAGAAAAGCTTAGTATAATGATTGAAGAGTG
  TGGAGGCTTGGATAAAATTGAAGCACTACAGAGGCATGAAAACGAGTCTGTATACAAGGCCTCATTGAAC
  TTAATTGAGAAGTACTTCTCAGTGGAGGAAGAGGAAGATCAAAATGTGGTGCCAGAAACTACCTCTGAAG
  GCTTCGCCTTCCAAGTTCAGGATGGAGCTCCTGGGACCTTTAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208477 representing BC003274
  Red=Cloning site Green=Tags(s)
  
  MSTNENANLPAARLNRFKNKGKDSTEMRRRRIEVNVELRKAKKDEQMLKRRNVSSFPDDATSPLQENRNN
  QGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGKTDCSPIQFES
  AWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLL
  ALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTD
  GPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTN
  IQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCG
  IIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENESVYKASLN
  LIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003274
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC003274.1
• RefSeq Size: 1962
• RefSeq ORF: 1589
• Locus ID: 16647
• MW: 71.9 kDa
• Gene Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. [UniProtKB/Swiss-Prot Function]